Cargando…
Investigation of Expression Profile of Placenta-specific 1 (PLAC1) in Acute Myeloid and Lymphoid Leukemias
BACKGROUND: Placenta-specific 1 (PLAC1) is one of the cancer-testis-placenta antigens that has no expression in normal tissue except placenta trophoblast and testicular germ cells, but is overexpressed in a variety of solid tumors. There is a lack of studies on the expression of PLAC1 in leukemia. W...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Avicenna Research Institute
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10395456/ https://www.ncbi.nlm.nih.gov/pubmed/37538244 http://dx.doi.org/10.18502/ajmb.v15i3.12926 |
_version_ | 1785083580523741184 |
---|---|
author | Gholami, Parastou Asgarian-Omran, Hossein Yaghmaie, Marjan Mahmudian, Jafar Kianersi, Shirin Salari, Sina Zaboli, Ehsan Jeddi-Tehrani, Mahmood Zarnani, Amir-Hassan Shabani, Mahdi |
author_facet | Gholami, Parastou Asgarian-Omran, Hossein Yaghmaie, Marjan Mahmudian, Jafar Kianersi, Shirin Salari, Sina Zaboli, Ehsan Jeddi-Tehrani, Mahmood Zarnani, Amir-Hassan Shabani, Mahdi |
author_sort | Gholami, Parastou |
collection | PubMed |
description | BACKGROUND: Placenta-specific 1 (PLAC1) is one of the cancer-testis-placenta antigens that has no expression in normal tissue except placenta trophoblast and testicular germ cells, but is overexpressed in a variety of solid tumors. There is a lack of studies on the expression of PLAC1 in leukemia. We investigated expression of PLAC1 in Acute Myeloid Leukemia (AML) and Acute Lymphoblastic Leukemia (ALL). METHODS: In this study, we investigated expression pattern of PLAC1 gene in peripheral blood and bone marrow mononuclear cells of newly-diagnosed patients with AML (n=31) and ALL (n=31) using quantitative real-time PCR. Normal subjects (n=17) were considered as control. The PLAC1 protein expression in the samples were also detected using western blotting. RESULTS: Our data demonstrated that PLAC1 transcripts had 2.7 and 2.9 fold-change increase in AML and ALL, respectively, compared to normal samples. PLAC1 transcript expression was totally negative in all studied normal subjects. Level of PLAC1 mRNA expression in ALL statistically increased compared to normal samples (p=0.038). However, relative mRNA expression of PLAC1 in AML was not significant in comparison to normal subjects (p=0.848). Furthermore, relative mRNA expression of PLAC1 in AML subtypes was not statistically significant (p=0.756). PLAC1 gene expression showed no difference in demographical clinical and para-clinical parameters. Western blotting confirmed expression of PLAC1 in the ALL and AML samples. CONCLUSION: Considering PLAC1 expression profile in acute leukemia, PLAC1 could be a potential marker in leukemia which needs complementary studies in the future. |
format | Online Article Text |
id | pubmed-10395456 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Avicenna Research Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-103954562023-08-03 Investigation of Expression Profile of Placenta-specific 1 (PLAC1) in Acute Myeloid and Lymphoid Leukemias Gholami, Parastou Asgarian-Omran, Hossein Yaghmaie, Marjan Mahmudian, Jafar Kianersi, Shirin Salari, Sina Zaboli, Ehsan Jeddi-Tehrani, Mahmood Zarnani, Amir-Hassan Shabani, Mahdi Avicenna J Med Biotechnol Original Article BACKGROUND: Placenta-specific 1 (PLAC1) is one of the cancer-testis-placenta antigens that has no expression in normal tissue except placenta trophoblast and testicular germ cells, but is overexpressed in a variety of solid tumors. There is a lack of studies on the expression of PLAC1 in leukemia. We investigated expression of PLAC1 in Acute Myeloid Leukemia (AML) and Acute Lymphoblastic Leukemia (ALL). METHODS: In this study, we investigated expression pattern of PLAC1 gene in peripheral blood and bone marrow mononuclear cells of newly-diagnosed patients with AML (n=31) and ALL (n=31) using quantitative real-time PCR. Normal subjects (n=17) were considered as control. The PLAC1 protein expression in the samples were also detected using western blotting. RESULTS: Our data demonstrated that PLAC1 transcripts had 2.7 and 2.9 fold-change increase in AML and ALL, respectively, compared to normal samples. PLAC1 transcript expression was totally negative in all studied normal subjects. Level of PLAC1 mRNA expression in ALL statistically increased compared to normal samples (p=0.038). However, relative mRNA expression of PLAC1 in AML was not significant in comparison to normal subjects (p=0.848). Furthermore, relative mRNA expression of PLAC1 in AML subtypes was not statistically significant (p=0.756). PLAC1 gene expression showed no difference in demographical clinical and para-clinical parameters. Western blotting confirmed expression of PLAC1 in the ALL and AML samples. CONCLUSION: Considering PLAC1 expression profile in acute leukemia, PLAC1 could be a potential marker in leukemia which needs complementary studies in the future. Avicenna Research Institute 2023 /pmc/articles/PMC10395456/ /pubmed/37538244 http://dx.doi.org/10.18502/ajmb.v15i3.12926 Text en Copyright© 2023 Avicenna Research Institute https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) |
spellingShingle | Original Article Gholami, Parastou Asgarian-Omran, Hossein Yaghmaie, Marjan Mahmudian, Jafar Kianersi, Shirin Salari, Sina Zaboli, Ehsan Jeddi-Tehrani, Mahmood Zarnani, Amir-Hassan Shabani, Mahdi Investigation of Expression Profile of Placenta-specific 1 (PLAC1) in Acute Myeloid and Lymphoid Leukemias |
title | Investigation of Expression Profile of Placenta-specific 1 (PLAC1) in Acute Myeloid and Lymphoid Leukemias |
title_full | Investigation of Expression Profile of Placenta-specific 1 (PLAC1) in Acute Myeloid and Lymphoid Leukemias |
title_fullStr | Investigation of Expression Profile of Placenta-specific 1 (PLAC1) in Acute Myeloid and Lymphoid Leukemias |
title_full_unstemmed | Investigation of Expression Profile of Placenta-specific 1 (PLAC1) in Acute Myeloid and Lymphoid Leukemias |
title_short | Investigation of Expression Profile of Placenta-specific 1 (PLAC1) in Acute Myeloid and Lymphoid Leukemias |
title_sort | investigation of expression profile of placenta-specific 1 (plac1) in acute myeloid and lymphoid leukemias |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10395456/ https://www.ncbi.nlm.nih.gov/pubmed/37538244 http://dx.doi.org/10.18502/ajmb.v15i3.12926 |
work_keys_str_mv | AT gholamiparastou investigationofexpressionprofileofplacentaspecific1plac1inacutemyeloidandlymphoidleukemias AT asgarianomranhossein investigationofexpressionprofileofplacentaspecific1plac1inacutemyeloidandlymphoidleukemias AT yaghmaiemarjan investigationofexpressionprofileofplacentaspecific1plac1inacutemyeloidandlymphoidleukemias AT mahmudianjafar investigationofexpressionprofileofplacentaspecific1plac1inacutemyeloidandlymphoidleukemias AT kianersishirin investigationofexpressionprofileofplacentaspecific1plac1inacutemyeloidandlymphoidleukemias AT salarisina investigationofexpressionprofileofplacentaspecific1plac1inacutemyeloidandlymphoidleukemias AT zaboliehsan investigationofexpressionprofileofplacentaspecific1plac1inacutemyeloidandlymphoidleukemias AT jedditehranimahmood investigationofexpressionprofileofplacentaspecific1plac1inacutemyeloidandlymphoidleukemias AT zarnaniamirhassan investigationofexpressionprofileofplacentaspecific1plac1inacutemyeloidandlymphoidleukemias AT shabanimahdi investigationofexpressionprofileofplacentaspecific1plac1inacutemyeloidandlymphoidleukemias |