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Synthesis and investigation of antiproliferative activity of Ru-NHC complexes against C6 and HeLa cancer cells

The 2-methylpyridine, 2-diethylaminoethyl, and isopentyl linked a series of symmetric and unsymmetric benzimidazolium salts 2a–e were prepared and used in the synthesis of silver-N-heterocyclic carbene (NHC) complexes (3a–e). The Ru(II)-NHC complexes (4a–h) were synthesized via transmetalation react...

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Autores principales: PAŞAHAN, Ramazan, AKKOÇ, Mitat, YAŞAR, Şeyma, KUL KÖPRÜLÜ, Tuğba, TEKİN, Şaban, YAŞAR, Sedat, ÖZDEMIR, İsmail
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Scientific and Technological Research Council of Turkey (TUBITAK) 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10395682/
https://www.ncbi.nlm.nih.gov/pubmed/37538752
http://dx.doi.org/10.55730/1300-0527.3418
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author PAŞAHAN, Ramazan
AKKOÇ, Mitat
YAŞAR, Şeyma
KUL KÖPRÜLÜ, Tuğba
TEKİN, Şaban
YAŞAR, Sedat
ÖZDEMIR, İsmail
author_facet PAŞAHAN, Ramazan
AKKOÇ, Mitat
YAŞAR, Şeyma
KUL KÖPRÜLÜ, Tuğba
TEKİN, Şaban
YAŞAR, Sedat
ÖZDEMIR, İsmail
author_sort PAŞAHAN, Ramazan
collection PubMed
description The 2-methylpyridine, 2-diethylaminoethyl, and isopentyl linked a series of symmetric and unsymmetric benzimidazolium salts 2a–e were prepared and used in the synthesis of silver-N-heterocyclic carbene (NHC) complexes (3a–e). The Ru(II)-NHC complexes (4a–h) were synthesized via transmetalation reaction from 3a–e. 4a–h complexes were converted to Ru(II)-NHC.HCl complexes (5ah) by HCl solution of diethyl ether and characterized by different spectroscopic techniques such as (1)H and (13)C NMR, LC/MS-Q-TOF, FT-IR, elemental analysis, and melting point detection. We examined the effect of the structural difference of complexes on anticancer activity via different arenes and metal centers. Antiproliferative activity of 5a–h and 3a was tested against human cervix adenocarcinoma (HeLa) and rat glioblastoma (C6) cell lines by ELISA assay. The IC(50) value of 5b, 5c and 5e complexes exhibited good cytotoxic activity than cisplatin on C6 (14.2 ± 0.5 mM; 16.2 ± 0.4 mM; 24.2 ± 0.7 mM, respectively) and HeLa (11.1 ± 0.5 mM; 13.7 ± 0.3 mM; 22.8 ± 0.8 mM, respectively) cell lines.
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spelling pubmed-103956822023-08-03 Synthesis and investigation of antiproliferative activity of Ru-NHC complexes against C6 and HeLa cancer cells PAŞAHAN, Ramazan AKKOÇ, Mitat YAŞAR, Şeyma KUL KÖPRÜLÜ, Tuğba TEKİN, Şaban YAŞAR, Sedat ÖZDEMIR, İsmail Turk J Chem Research Article The 2-methylpyridine, 2-diethylaminoethyl, and isopentyl linked a series of symmetric and unsymmetric benzimidazolium salts 2a–e were prepared and used in the synthesis of silver-N-heterocyclic carbene (NHC) complexes (3a–e). The Ru(II)-NHC complexes (4a–h) were synthesized via transmetalation reaction from 3a–e. 4a–h complexes were converted to Ru(II)-NHC.HCl complexes (5ah) by HCl solution of diethyl ether and characterized by different spectroscopic techniques such as (1)H and (13)C NMR, LC/MS-Q-TOF, FT-IR, elemental analysis, and melting point detection. We examined the effect of the structural difference of complexes on anticancer activity via different arenes and metal centers. Antiproliferative activity of 5a–h and 3a was tested against human cervix adenocarcinoma (HeLa) and rat glioblastoma (C6) cell lines by ELISA assay. The IC(50) value of 5b, 5c and 5e complexes exhibited good cytotoxic activity than cisplatin on C6 (14.2 ± 0.5 mM; 16.2 ± 0.4 mM; 24.2 ± 0.7 mM, respectively) and HeLa (11.1 ± 0.5 mM; 13.7 ± 0.3 mM; 22.8 ± 0.8 mM, respectively) cell lines. Scientific and Technological Research Council of Turkey (TUBITAK) 2022-03-15 /pmc/articles/PMC10395682/ /pubmed/37538752 http://dx.doi.org/10.55730/1300-0527.3418 Text en © TÜBİTAK https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License.
spellingShingle Research Article
PAŞAHAN, Ramazan
AKKOÇ, Mitat
YAŞAR, Şeyma
KUL KÖPRÜLÜ, Tuğba
TEKİN, Şaban
YAŞAR, Sedat
ÖZDEMIR, İsmail
Synthesis and investigation of antiproliferative activity of Ru-NHC complexes against C6 and HeLa cancer cells
title Synthesis and investigation of antiproliferative activity of Ru-NHC complexes against C6 and HeLa cancer cells
title_full Synthesis and investigation of antiproliferative activity of Ru-NHC complexes against C6 and HeLa cancer cells
title_fullStr Synthesis and investigation of antiproliferative activity of Ru-NHC complexes against C6 and HeLa cancer cells
title_full_unstemmed Synthesis and investigation of antiproliferative activity of Ru-NHC complexes against C6 and HeLa cancer cells
title_short Synthesis and investigation of antiproliferative activity of Ru-NHC complexes against C6 and HeLa cancer cells
title_sort synthesis and investigation of antiproliferative activity of ru-nhc complexes against c6 and hela cancer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10395682/
https://www.ncbi.nlm.nih.gov/pubmed/37538752
http://dx.doi.org/10.55730/1300-0527.3418
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