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Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development

Mineralized tissues, such as bones or teeth, are essential structures of all vertebrates. They enable rapid movement, protection, and food processing, in addition to providing physiological functions. Although the development, regeneration, and pathogenesis of teeth and bones have been intensely stu...

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Autores principales: Gonzalez Lopez, Marcos, Huteckova, Barbora, Lavicky, Josef, Zezula, Nikodem, Rakultsev, Vladislav, Fridrichova, Vendula, Tuaima, Haneen, Nottmeier, Cita, Petersen, Julian, Kavkova, Michaela, Zikmund, Tomas, Kaiser, Jozef, Lav, Rupali, Star, Haza, Bryja, Vítězslav, Henyš, Petr, Vořechovský, Miroslav, Tucker, Abigail S., Harnos, Jakub, Buchtova, Marcela, Krivanek, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10396306/
https://www.ncbi.nlm.nih.gov/pubmed/37531427
http://dx.doi.org/10.1126/sciadv.adi0482
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author Gonzalez Lopez, Marcos
Huteckova, Barbora
Lavicky, Josef
Zezula, Nikodem
Rakultsev, Vladislav
Fridrichova, Vendula
Tuaima, Haneen
Nottmeier, Cita
Petersen, Julian
Kavkova, Michaela
Zikmund, Tomas
Kaiser, Jozef
Lav, Rupali
Star, Haza
Bryja, Vítězslav
Henyš, Petr
Vořechovský, Miroslav
Tucker, Abigail S.
Harnos, Jakub
Buchtova, Marcela
Krivanek, Jan
author_facet Gonzalez Lopez, Marcos
Huteckova, Barbora
Lavicky, Josef
Zezula, Nikodem
Rakultsev, Vladislav
Fridrichova, Vendula
Tuaima, Haneen
Nottmeier, Cita
Petersen, Julian
Kavkova, Michaela
Zikmund, Tomas
Kaiser, Jozef
Lav, Rupali
Star, Haza
Bryja, Vítězslav
Henyš, Petr
Vořechovský, Miroslav
Tucker, Abigail S.
Harnos, Jakub
Buchtova, Marcela
Krivanek, Jan
author_sort Gonzalez Lopez, Marcos
collection PubMed
description Mineralized tissues, such as bones or teeth, are essential structures of all vertebrates. They enable rapid movement, protection, and food processing, in addition to providing physiological functions. Although the development, regeneration, and pathogenesis of teeth and bones have been intensely studied, there is currently no tool to accurately follow the dynamics of growth and healing of these vital tissues in space and time. Here, we present the BEE-ST (Bones and tEEth Spatio-Temporal growth monitoring) approach, which allows precise quantification of development, regeneration, remodeling, and healing in any type of calcified tissue across different species. Using mouse teeth as model the turnover rate of continuously growing incisors was quantified, and role of hard/soft diet on molar root growth was shown. Furthermore, the dynamics of bones and teeth growth in lizards, frogs, birds, and zebrafish was uncovered. This approach represents an effective, highly reproducible, and versatile tool that opens up diverse possibilities in developmental biology, bone and tooth healing, tissue engineering, and disease modeling.
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spelling pubmed-103963062023-08-03 Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development Gonzalez Lopez, Marcos Huteckova, Barbora Lavicky, Josef Zezula, Nikodem Rakultsev, Vladislav Fridrichova, Vendula Tuaima, Haneen Nottmeier, Cita Petersen, Julian Kavkova, Michaela Zikmund, Tomas Kaiser, Jozef Lav, Rupali Star, Haza Bryja, Vítězslav Henyš, Petr Vořechovský, Miroslav Tucker, Abigail S. Harnos, Jakub Buchtova, Marcela Krivanek, Jan Sci Adv Biomedicine and Life Sciences Mineralized tissues, such as bones or teeth, are essential structures of all vertebrates. They enable rapid movement, protection, and food processing, in addition to providing physiological functions. Although the development, regeneration, and pathogenesis of teeth and bones have been intensely studied, there is currently no tool to accurately follow the dynamics of growth and healing of these vital tissues in space and time. Here, we present the BEE-ST (Bones and tEEth Spatio-Temporal growth monitoring) approach, which allows precise quantification of development, regeneration, remodeling, and healing in any type of calcified tissue across different species. Using mouse teeth as model the turnover rate of continuously growing incisors was quantified, and role of hard/soft diet on molar root growth was shown. Furthermore, the dynamics of bones and teeth growth in lizards, frogs, birds, and zebrafish was uncovered. This approach represents an effective, highly reproducible, and versatile tool that opens up diverse possibilities in developmental biology, bone and tooth healing, tissue engineering, and disease modeling. American Association for the Advancement of Science 2023-08-02 /pmc/articles/PMC10396306/ /pubmed/37531427 http://dx.doi.org/10.1126/sciadv.adi0482 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Biomedicine and Life Sciences
Gonzalez Lopez, Marcos
Huteckova, Barbora
Lavicky, Josef
Zezula, Nikodem
Rakultsev, Vladislav
Fridrichova, Vendula
Tuaima, Haneen
Nottmeier, Cita
Petersen, Julian
Kavkova, Michaela
Zikmund, Tomas
Kaiser, Jozef
Lav, Rupali
Star, Haza
Bryja, Vítězslav
Henyš, Petr
Vořechovský, Miroslav
Tucker, Abigail S.
Harnos, Jakub
Buchtova, Marcela
Krivanek, Jan
Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development
title Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development
title_full Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development
title_fullStr Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development
title_full_unstemmed Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development
title_short Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development
title_sort spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development
topic Biomedicine and Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10396306/
https://www.ncbi.nlm.nih.gov/pubmed/37531427
http://dx.doi.org/10.1126/sciadv.adi0482
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