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RETICULON-LIKE PROTEIN B2 is a proviral factor co-opted for the biogenesis of viral replication organelles in plants

Endomembrane remodeling to form a viral replication complex (VRC) is crucial for a virus to establish infection in a host. Although the composition and function of VRCs have been intensively studied, host factors involved in the assembly of VRCs for plant RNA viruses have not been fully explored. Tu...

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Autores principales: Zhang, Qianshen, Wen, Zhiyan, Zhang, Xin, She, Jiajie, Wang, Xiaoling, Gao, Zongyu, Wang, Ruiqi, Zhao, Xiaofei, Su, Zhen, Li, Zhen, Li, Dawei, Wang, Xiaofeng, Zhang, Yongliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10396372/
https://www.ncbi.nlm.nih.gov/pubmed/37216674
http://dx.doi.org/10.1093/plcell/koad146
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author Zhang, Qianshen
Wen, Zhiyan
Zhang, Xin
She, Jiajie
Wang, Xiaoling
Gao, Zongyu
Wang, Ruiqi
Zhao, Xiaofei
Su, Zhen
Li, Zhen
Li, Dawei
Wang, Xiaofeng
Zhang, Yongliang
author_facet Zhang, Qianshen
Wen, Zhiyan
Zhang, Xin
She, Jiajie
Wang, Xiaoling
Gao, Zongyu
Wang, Ruiqi
Zhao, Xiaofei
Su, Zhen
Li, Zhen
Li, Dawei
Wang, Xiaofeng
Zhang, Yongliang
author_sort Zhang, Qianshen
collection PubMed
description Endomembrane remodeling to form a viral replication complex (VRC) is crucial for a virus to establish infection in a host. Although the composition and function of VRCs have been intensively studied, host factors involved in the assembly of VRCs for plant RNA viruses have not been fully explored. TurboID-based proximity labeling (PL) has emerged as a robust tool for probing molecular interactions in planta. However, few studies have employed the TurboID-based PL technique for investigating plant virus replication. Here, we used Beet black scorch virus (BBSV), an endoplasmic reticulum (ER)–replicating virus, as a model and systematically investigated the composition of BBSV VRCs in Nicotiana benthamiana by fusing the TurboID enzyme to viral replication protein p23. Among the 185 identified p23-proximal proteins, the reticulon family of proteins showed high reproducibility in the mass spectrometry data sets. We focused on RETICULON-LIKE PROTEIN B2 (RTNLB2) and demonstrated its proviral functions in BBSV replication. We showed that RTNLB2 binds to p23, induces ER membrane curvature, and constricts ER tubules to facilitate the assembly of BBSV VRCs. Our comprehensive proximal interactome analysis of BBSV VRCs provides a resource for understanding plant viral replication and offers additional insights into the formation of membrane scaffolds for viral RNA synthesis.
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spelling pubmed-103963722023-08-03 RETICULON-LIKE PROTEIN B2 is a proviral factor co-opted for the biogenesis of viral replication organelles in plants Zhang, Qianshen Wen, Zhiyan Zhang, Xin She, Jiajie Wang, Xiaoling Gao, Zongyu Wang, Ruiqi Zhao, Xiaofei Su, Zhen Li, Zhen Li, Dawei Wang, Xiaofeng Zhang, Yongliang Plant Cell Research Article Endomembrane remodeling to form a viral replication complex (VRC) is crucial for a virus to establish infection in a host. Although the composition and function of VRCs have been intensively studied, host factors involved in the assembly of VRCs for plant RNA viruses have not been fully explored. TurboID-based proximity labeling (PL) has emerged as a robust tool for probing molecular interactions in planta. However, few studies have employed the TurboID-based PL technique for investigating plant virus replication. Here, we used Beet black scorch virus (BBSV), an endoplasmic reticulum (ER)–replicating virus, as a model and systematically investigated the composition of BBSV VRCs in Nicotiana benthamiana by fusing the TurboID enzyme to viral replication protein p23. Among the 185 identified p23-proximal proteins, the reticulon family of proteins showed high reproducibility in the mass spectrometry data sets. We focused on RETICULON-LIKE PROTEIN B2 (RTNLB2) and demonstrated its proviral functions in BBSV replication. We showed that RTNLB2 binds to p23, induces ER membrane curvature, and constricts ER tubules to facilitate the assembly of BBSV VRCs. Our comprehensive proximal interactome analysis of BBSV VRCs provides a resource for understanding plant viral replication and offers additional insights into the formation of membrane scaffolds for viral RNA synthesis. Oxford University Press 2023-05-22 /pmc/articles/PMC10396372/ /pubmed/37216674 http://dx.doi.org/10.1093/plcell/koad146 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Article
Zhang, Qianshen
Wen, Zhiyan
Zhang, Xin
She, Jiajie
Wang, Xiaoling
Gao, Zongyu
Wang, Ruiqi
Zhao, Xiaofei
Su, Zhen
Li, Zhen
Li, Dawei
Wang, Xiaofeng
Zhang, Yongliang
RETICULON-LIKE PROTEIN B2 is a proviral factor co-opted for the biogenesis of viral replication organelles in plants
title RETICULON-LIKE PROTEIN B2 is a proviral factor co-opted for the biogenesis of viral replication organelles in plants
title_full RETICULON-LIKE PROTEIN B2 is a proviral factor co-opted for the biogenesis of viral replication organelles in plants
title_fullStr RETICULON-LIKE PROTEIN B2 is a proviral factor co-opted for the biogenesis of viral replication organelles in plants
title_full_unstemmed RETICULON-LIKE PROTEIN B2 is a proviral factor co-opted for the biogenesis of viral replication organelles in plants
title_short RETICULON-LIKE PROTEIN B2 is a proviral factor co-opted for the biogenesis of viral replication organelles in plants
title_sort reticulon-like protein b2 is a proviral factor co-opted for the biogenesis of viral replication organelles in plants
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10396372/
https://www.ncbi.nlm.nih.gov/pubmed/37216674
http://dx.doi.org/10.1093/plcell/koad146
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