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FARL-11 (STRIP1/2) is required for sarcomere and sarcoplasmic reticulum organization in C. elegans
Protein phosphatase 2A (PP2A) functions in a variety of cellular contexts. PP2A can assemble into four different complexes based on the inclusion of different regulatory or targeting subunits. The B’’’ regulatory subunit “striatin” forms the STRIPAK complex consisting of striatin, a catalytic subuni...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10398898/ https://www.ncbi.nlm.nih.gov/pubmed/37314837 http://dx.doi.org/10.1091/mbc.E23-03-0083 |
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author | Martin, Sterling C. T. Qadota, Hiroshi Oberhauser, Andres F. Hardin, Jeff Benian, Guy M. |
author_facet | Martin, Sterling C. T. Qadota, Hiroshi Oberhauser, Andres F. Hardin, Jeff Benian, Guy M. |
author_sort | Martin, Sterling C. T. |
collection | PubMed |
description | Protein phosphatase 2A (PP2A) functions in a variety of cellular contexts. PP2A can assemble into four different complexes based on the inclusion of different regulatory or targeting subunits. The B’’’ regulatory subunit “striatin” forms the STRIPAK complex consisting of striatin, a catalytic subunit (PP2AC), striatin-interacting protein 1 (STRIP1), and MOB family member 4 (MOB4). In yeast and Caenorhabditis elegans, STRIP1 is required for formation of the endoplasmic reticulum (ER). Because the sarcoplasmic reticulum (SR) is the highly organized muscle-specific version of ER, we sought to determine the function of the STRIPAK complex in muscle using C. elegans. CASH-1 (striatin) and FARL-11 (STRIP1/2) form a complex in vivo, and each protein is localized to SR. Missense mutations and single amino acid losses in farl-11 and cash-1 each result in similar sarcomere disorganization. A missense mutation in farl-11 shows no detectable FARL-11 protein by immunoblot, disruption of SR organization around M-lines, and altered levels of the SR Ca(+2) release channel UNC-68. |
format | Online Article Text |
id | pubmed-10398898 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-103988982023-10-16 FARL-11 (STRIP1/2) is required for sarcomere and sarcoplasmic reticulum organization in C. elegans Martin, Sterling C. T. Qadota, Hiroshi Oberhauser, Andres F. Hardin, Jeff Benian, Guy M. Mol Biol Cell Articles Protein phosphatase 2A (PP2A) functions in a variety of cellular contexts. PP2A can assemble into four different complexes based on the inclusion of different regulatory or targeting subunits. The B’’’ regulatory subunit “striatin” forms the STRIPAK complex consisting of striatin, a catalytic subunit (PP2AC), striatin-interacting protein 1 (STRIP1), and MOB family member 4 (MOB4). In yeast and Caenorhabditis elegans, STRIP1 is required for formation of the endoplasmic reticulum (ER). Because the sarcoplasmic reticulum (SR) is the highly organized muscle-specific version of ER, we sought to determine the function of the STRIPAK complex in muscle using C. elegans. CASH-1 (striatin) and FARL-11 (STRIP1/2) form a complex in vivo, and each protein is localized to SR. Missense mutations and single amino acid losses in farl-11 and cash-1 each result in similar sarcomere disorganization. A missense mutation in farl-11 shows no detectable FARL-11 protein by immunoblot, disruption of SR organization around M-lines, and altered levels of the SR Ca(+2) release channel UNC-68. The American Society for Cell Biology 2023-08-01 /pmc/articles/PMC10398898/ /pubmed/37314837 http://dx.doi.org/10.1091/mbc.E23-03-0083 Text en © 2023 Martin et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial-Share Alike 4.0 International Creative Commons License. |
spellingShingle | Articles Martin, Sterling C. T. Qadota, Hiroshi Oberhauser, Andres F. Hardin, Jeff Benian, Guy M. FARL-11 (STRIP1/2) is required for sarcomere and sarcoplasmic reticulum organization in C. elegans |
title | FARL-11 (STRIP1/2) is required for sarcomere and sarcoplasmic reticulum organization in C. elegans |
title_full | FARL-11 (STRIP1/2) is required for sarcomere and sarcoplasmic reticulum organization in C. elegans |
title_fullStr | FARL-11 (STRIP1/2) is required for sarcomere and sarcoplasmic reticulum organization in C. elegans |
title_full_unstemmed | FARL-11 (STRIP1/2) is required for sarcomere and sarcoplasmic reticulum organization in C. elegans |
title_short | FARL-11 (STRIP1/2) is required for sarcomere and sarcoplasmic reticulum organization in C. elegans |
title_sort | farl-11 (strip1/2) is required for sarcomere and sarcoplasmic reticulum organization in c. elegans |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10398898/ https://www.ncbi.nlm.nih.gov/pubmed/37314837 http://dx.doi.org/10.1091/mbc.E23-03-0083 |
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