Transcriptome profiling analysis of uterus during chicken laying periods

The avian eggshell is formed in the uterus. Changes in uterine function may have a significant effect on eggshell quality. To identify the vital genes impacting uterine functional maintenance in the chicken, uteri in three different periods (22W, 31W, 51W) were selected for RNA sequencing and bioinf...

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Autores principales: Sun, Tiantian, Xiao, Cong, Yang, Zhuliang, Deng, Jixian, Yang, Xiurong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10398974/
https://www.ncbi.nlm.nih.gov/pubmed/37537566
http://dx.doi.org/10.1186/s12864-023-09521-z
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author Sun, Tiantian
Xiao, Cong
Yang, Zhuliang
Deng, Jixian
Yang, Xiurong
author_facet Sun, Tiantian
Xiao, Cong
Yang, Zhuliang
Deng, Jixian
Yang, Xiurong
author_sort Sun, Tiantian
collection PubMed
description The avian eggshell is formed in the uterus. Changes in uterine function may have a significant effect on eggshell quality. To identify the vital genes impacting uterine functional maintenance in the chicken, uteri in three different periods (22W, 31W, 51W) were selected for RNA sequencing and bioinformatics analysis. In our study, 520, 706 and 736 differentially expressed genes (DEGs) were respectively detected in the W31 vs W22 group, W51 vs W31 group and W51 vs W22 group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated DEGs were enriched in the extracellular matrix, extracellular region part, extracellular region, extracellular matrix structural constituent, ECM receptor interaction, collagen-containing extracellular matrix and collagen trimer in the uterus (P < 0.05). Protein–protein interaction analysis revealed that FN1, LOX, THBS2, COL1A1, COL1A2, COL5A1, COL5A2, POSTN, MMP13, VANGL2, RAD54B, SPP1, SDC1, BTC, ANGPTL3 might be key candidate genes for uterine functional maintenance in chicken. This study discovered dominant genes and pathways which enhanced our knowledge of chicken uterine functional maintenance. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09521-z.
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spelling pubmed-103989742023-08-04 Transcriptome profiling analysis of uterus during chicken laying periods Sun, Tiantian Xiao, Cong Yang, Zhuliang Deng, Jixian Yang, Xiurong BMC Genomics Research The avian eggshell is formed in the uterus. Changes in uterine function may have a significant effect on eggshell quality. To identify the vital genes impacting uterine functional maintenance in the chicken, uteri in three different periods (22W, 31W, 51W) were selected for RNA sequencing and bioinformatics analysis. In our study, 520, 706 and 736 differentially expressed genes (DEGs) were respectively detected in the W31 vs W22 group, W51 vs W31 group and W51 vs W22 group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated DEGs were enriched in the extracellular matrix, extracellular region part, extracellular region, extracellular matrix structural constituent, ECM receptor interaction, collagen-containing extracellular matrix and collagen trimer in the uterus (P < 0.05). Protein–protein interaction analysis revealed that FN1, LOX, THBS2, COL1A1, COL1A2, COL5A1, COL5A2, POSTN, MMP13, VANGL2, RAD54B, SPP1, SDC1, BTC, ANGPTL3 might be key candidate genes for uterine functional maintenance in chicken. This study discovered dominant genes and pathways which enhanced our knowledge of chicken uterine functional maintenance. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09521-z. BioMed Central 2023-08-03 /pmc/articles/PMC10398974/ /pubmed/37537566 http://dx.doi.org/10.1186/s12864-023-09521-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Sun, Tiantian
Xiao, Cong
Yang, Zhuliang
Deng, Jixian
Yang, Xiurong
Transcriptome profiling analysis of uterus during chicken laying periods
title Transcriptome profiling analysis of uterus during chicken laying periods
title_full Transcriptome profiling analysis of uterus during chicken laying periods
title_fullStr Transcriptome profiling analysis of uterus during chicken laying periods
title_full_unstemmed Transcriptome profiling analysis of uterus during chicken laying periods
title_short Transcriptome profiling analysis of uterus during chicken laying periods
title_sort transcriptome profiling analysis of uterus during chicken laying periods
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10398974/
https://www.ncbi.nlm.nih.gov/pubmed/37537566
http://dx.doi.org/10.1186/s12864-023-09521-z
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