One-Pot Synthesis of Nitrogen-Doped Graphene Quantum Dots and Their Applications in Bioimaging and Detecting Copper Ions in Living Cells

[Image: see text] Two natural carbon sources, glutamic acid and tyrosine, were used to fabricate strong green emission nitrogen-doped graphene quantum dots (N-GQDs) with the one-pot pyrolysis method. The morphology of the prepared GQDs has been characterized by high-resolution transmission electron microscopy, showing a well-displayed crystalline structure with a lattice spacing of 0.262 nm. X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy were used to analyze the surface functional groups and elemental composition, suggesting that the N-GQDs have active carboxylic and amino functional groups. Meanwhile, photoluminescence and ultraviolet–visible (UV–vis) spectroscopy were used to evaluate the optical properties of GQDs; the prepared N-GQDs show an excitation-dependent fluorescence behavior with a maximum excitation/emission wavelength at 460/522 nm, respectively. N-GQDs showed good photostability and the fluorescence intensity quenched about 10% after irradiating 2800 s in the experiment of time kinetic analysis. The MTT assay was utilized to assess the viability of N-GQDs; good biocompatibility with a relatively high quantum yield of 12% demonstrated the potential for serving as bioimaging agents. Besides, the selectivity study on metal ions indicates that the N-GQDs could be used in Cu(2+) detection. The linear range is from 0.1 to 10 μM with a limit of detection of 0.06 μM. Overall, these proposed N-GQDs with one-pot synthesis showed their promising potential in cell imaging and Cu(2+) monitoring applications involved in the biological environment.