SNHG15 aids SARS-CoV-2 entry via RABL2A

Angiotensin-converting enzyme 2 (ACE2) and several proteins have been identified as entry factors for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, whether long noncoding RNAs are involved in SARS-CoV-2 entry remains unknown. In this study, we investigated the role of small...

Descripción completa

Detalles Bibliográficos
Autores principales: Pushparaj, Samuel, Gandikota, Chaitanya, Vaddadi, Kishore, Liang, Yurong, Liu, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2023
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10399485/
https://www.ncbi.nlm.nih.gov/pubmed/37528621
http://dx.doi.org/10.1080/15476286.2023.2241755
_version_ 1785084256429539328
author Pushparaj, Samuel
Gandikota, Chaitanya
Vaddadi, Kishore
Liang, Yurong
Liu, Lin
author_facet Pushparaj, Samuel
Gandikota, Chaitanya
Vaddadi, Kishore
Liang, Yurong
Liu, Lin
author_sort Pushparaj, Samuel
collection PubMed
description Angiotensin-converting enzyme 2 (ACE2) and several proteins have been identified as entry factors for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, whether long noncoding RNAs are involved in SARS-CoV-2 entry remains unknown. In this study, we investigated the role of small nucleolar RNA host gene 15 (SNHG15) in SARS-CoV-2 entry using a SARS-CoV-2 spike pseudotyped lentivirus with a luciferase reporter. Overexpression of SNHG15 promoted but SNHG15 knockdown limited SARS-CoV-2 entry in a dose- and time-dependent manner. SNHG15 interacted with Rab-like protein 2A (RABL2A). Overexpression and knockdown of RABL2A produced similar effects on SARS-CoV-2 entry as those of SNHG15. Furthermore, RABL2A knockdown abolished the SNHG15-mediated increase in SARS-CoV-2 entry. In conclusion, SNHG15 is a critical regulatory factor that aids SARS-CoV-2 entry through RABL2A.
format Online
Article
Text
id pubmed-10399485
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-103994852023-08-04 SNHG15 aids SARS-CoV-2 entry via RABL2A Pushparaj, Samuel Gandikota, Chaitanya Vaddadi, Kishore Liang, Yurong Liu, Lin RNA Biol Brief Communication Angiotensin-converting enzyme 2 (ACE2) and several proteins have been identified as entry factors for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, whether long noncoding RNAs are involved in SARS-CoV-2 entry remains unknown. In this study, we investigated the role of small nucleolar RNA host gene 15 (SNHG15) in SARS-CoV-2 entry using a SARS-CoV-2 spike pseudotyped lentivirus with a luciferase reporter. Overexpression of SNHG15 promoted but SNHG15 knockdown limited SARS-CoV-2 entry in a dose- and time-dependent manner. SNHG15 interacted with Rab-like protein 2A (RABL2A). Overexpression and knockdown of RABL2A produced similar effects on SARS-CoV-2 entry as those of SNHG15. Furthermore, RABL2A knockdown abolished the SNHG15-mediated increase in SARS-CoV-2 entry. In conclusion, SNHG15 is a critical regulatory factor that aids SARS-CoV-2 entry through RABL2A. Taylor & Francis 2023-08-01 /pmc/articles/PMC10399485/ /pubmed/37528621 http://dx.doi.org/10.1080/15476286.2023.2241755 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.
spellingShingle Brief Communication
Pushparaj, Samuel
Gandikota, Chaitanya
Vaddadi, Kishore
Liang, Yurong
Liu, Lin
SNHG15 aids SARS-CoV-2 entry via RABL2A
title SNHG15 aids SARS-CoV-2 entry via RABL2A
title_full SNHG15 aids SARS-CoV-2 entry via RABL2A
title_fullStr SNHG15 aids SARS-CoV-2 entry via RABL2A
title_full_unstemmed SNHG15 aids SARS-CoV-2 entry via RABL2A
title_short SNHG15 aids SARS-CoV-2 entry via RABL2A
title_sort snhg15 aids sars-cov-2 entry via rabl2a
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10399485/
https://www.ncbi.nlm.nih.gov/pubmed/37528621
http://dx.doi.org/10.1080/15476286.2023.2241755
work_keys_str_mv AT pushparajsamuel snhg15aidssarscov2entryviarabl2a
AT gandikotachaitanya snhg15aidssarscov2entryviarabl2a
AT vaddadikishore snhg15aidssarscov2entryviarabl2a
AT liangyurong snhg15aidssarscov2entryviarabl2a
AT liulin snhg15aidssarscov2entryviarabl2a

Ejemplares similares