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Neorickettsia sp. in coatis (Nasua nasua) in Brazil
The genus Neorickettsia comprises trematode-associated bacteria that can cause diseases in animals and humans. Despite detection of Neorickettsia antigens in the intestine of coatis kept in captivity in southern Brazil through immunohistochemistry, the molecular identity of the bacteria in South Ame...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Colégio Brasileiro de Parasitologia Veterinária
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10399552/ https://www.ncbi.nlm.nih.gov/pubmed/37466626 http://dx.doi.org/10.1590/S1984-29612023042 |
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author | Perles, Lívia Barreto, Wanessa Teixeira Gomes de Macedo, Gabriel Carvalho Herrera, Heitor Miraglia Machado, Rosangela Zacarias André, Marcos Rogério |
author_facet | Perles, Lívia Barreto, Wanessa Teixeira Gomes de Macedo, Gabriel Carvalho Herrera, Heitor Miraglia Machado, Rosangela Zacarias André, Marcos Rogério |
author_sort | Perles, Lívia |
collection | PubMed |
description | The genus Neorickettsia comprises trematode-associated bacteria that can cause diseases in animals and humans. Despite detection of Neorickettsia antigens in the intestine of coatis kept in captivity in southern Brazil through immunohistochemistry, the molecular identity of the bacteria in South American procyonids remains elusive. The aim of the present study was to investigate the occurrence of Neorickettsia sp. in blood samples from coatis in central-western Brazil. Between March 2018 and January 2019, animals were captured and recaptured in two areas of the Cerrado (Parque Estadual do Prosa, PEP; and Vila da Base Aérea, VBA) located in the city of Campo Grande, state of Mato Grosso do Sul, central-western Brazil. All captures were performed according to convenience. DNA from 97 blood samples was subjected to nested PCR (nPCR) targeting a fragment of the 16S rRNA gene of Neorickettsia sp. Six samples (3.6%; five from VBA and one from PEP) from different coatis were positive in nPCR based on the 16S rRNA. The sequences obtained (~500 bp) showed ˃ 99% similarity to N. risticii. Phylogenetic analysis clustered the sequences detected in the present study in a clade with N. risticii. This is the first molecular detection of Neorickettsia sp. in coatis in Brazil. |
format | Online Article Text |
id | pubmed-10399552 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Colégio Brasileiro de Parasitologia Veterinária |
record_format | MEDLINE/PubMed |
spelling | pubmed-103995522023-08-04 Neorickettsia sp. in coatis (Nasua nasua) in Brazil Perles, Lívia Barreto, Wanessa Teixeira Gomes de Macedo, Gabriel Carvalho Herrera, Heitor Miraglia Machado, Rosangela Zacarias André, Marcos Rogério Rev Bras Parasitol Vet Short Communication The genus Neorickettsia comprises trematode-associated bacteria that can cause diseases in animals and humans. Despite detection of Neorickettsia antigens in the intestine of coatis kept in captivity in southern Brazil through immunohistochemistry, the molecular identity of the bacteria in South American procyonids remains elusive. The aim of the present study was to investigate the occurrence of Neorickettsia sp. in blood samples from coatis in central-western Brazil. Between March 2018 and January 2019, animals were captured and recaptured in two areas of the Cerrado (Parque Estadual do Prosa, PEP; and Vila da Base Aérea, VBA) located in the city of Campo Grande, state of Mato Grosso do Sul, central-western Brazil. All captures were performed according to convenience. DNA from 97 blood samples was subjected to nested PCR (nPCR) targeting a fragment of the 16S rRNA gene of Neorickettsia sp. Six samples (3.6%; five from VBA and one from PEP) from different coatis were positive in nPCR based on the 16S rRNA. The sequences obtained (~500 bp) showed ˃ 99% similarity to N. risticii. Phylogenetic analysis clustered the sequences detected in the present study in a clade with N. risticii. This is the first molecular detection of Neorickettsia sp. in coatis in Brazil. Colégio Brasileiro de Parasitologia Veterinária 2023-07-17 /pmc/articles/PMC10399552/ /pubmed/37466626 http://dx.doi.org/10.1590/S1984-29612023042 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Perles, Lívia Barreto, Wanessa Teixeira Gomes de Macedo, Gabriel Carvalho Herrera, Heitor Miraglia Machado, Rosangela Zacarias André, Marcos Rogério Neorickettsia sp. in coatis (Nasua nasua) in Brazil |
title | Neorickettsia sp. in coatis (Nasua nasua) in Brazil |
title_full | Neorickettsia sp. in coatis (Nasua nasua) in Brazil |
title_fullStr | Neorickettsia sp. in coatis (Nasua nasua) in Brazil |
title_full_unstemmed | Neorickettsia sp. in coatis (Nasua nasua) in Brazil |
title_short | Neorickettsia sp. in coatis (Nasua nasua) in Brazil |
title_sort | neorickettsia sp. in coatis (nasua nasua) in brazil |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10399552/ https://www.ncbi.nlm.nih.gov/pubmed/37466626 http://dx.doi.org/10.1590/S1984-29612023042 |
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