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Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity

Melanins are complex pigments with various biological functions and potential applications in space exploration and biomedicine due to their radioprotective properties. Aspergillus niger, a fungus known for its high radiation resistance, is widely used in biotechnology and a candidate for melanin pr...

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Autores principales: Koch, Stella Marie, Freidank-Pohl, Carsten, Siontas, Oliver, Cortesao, Marta, Mota, Afonso, Runzheimer, Katharina, Jung, Sascha, Rebrosova, Katarina, Siler, Martin, Moeller, Ralf, Meyer, Vera
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10399693/
https://www.ncbi.nlm.nih.gov/pubmed/37547691
http://dx.doi.org/10.3389/fmicb.2023.1233740
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author Koch, Stella Marie
Freidank-Pohl, Carsten
Siontas, Oliver
Cortesao, Marta
Mota, Afonso
Runzheimer, Katharina
Jung, Sascha
Rebrosova, Katarina
Siler, Martin
Moeller, Ralf
Meyer, Vera
author_facet Koch, Stella Marie
Freidank-Pohl, Carsten
Siontas, Oliver
Cortesao, Marta
Mota, Afonso
Runzheimer, Katharina
Jung, Sascha
Rebrosova, Katarina
Siler, Martin
Moeller, Ralf
Meyer, Vera
author_sort Koch, Stella Marie
collection PubMed
description Melanins are complex pigments with various biological functions and potential applications in space exploration and biomedicine due to their radioprotective properties. Aspergillus niger, a fungus known for its high radiation resistance, is widely used in biotechnology and a candidate for melanin production. In this study, we investigated the production of fungal pyomelanin (Pyo(Fun)) in A. niger by inducing overproduction of the pigment using L-tyrosine in a recombinant ΔhmgA mutant strain (OS4.3). The Pyo(Fun) pigment was characterized using three spectroscopic methods, and its antioxidant properties were assessed using a DPPH-assay. Additionally, we evaluated the protective effect of Pyo(Fun) against non-ionizing radiation (monochromatic UV-C) and compared its efficacy to a synthetically produced control pyomelanin (Pyo(Syn)). The results confirmed successful production of Pyo(Fun) in A. niger through inducible overproduction. Characterization using spectroscopic methods confirmed the presence of Pyo(Fun), and the DPPH-assay demonstrated its strong antioxidant properties. Moreover, Pyo(Fun) exhibited a highly protective effect against radiation-induced stress, surpassing the protection provided by Pyo(Syn). The findings of this study suggest that Pyo(Fun) has significant potential as a biological shield against harmful radiation. Notably, Pyo(Fun) is synthesized extracellularly, differing it from other fungal melanins (such as L-DOPA- or DHN-melanin) that require cell lysis for pigment purification. This characteristic makes Pyo(Fun) a valuable resource for biotechnology, biomedicine, and the space industry. However, further research is needed to evaluate its protective effect in a dried form and against ionizing radiation.
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spelling pubmed-103996932023-08-04 Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity Koch, Stella Marie Freidank-Pohl, Carsten Siontas, Oliver Cortesao, Marta Mota, Afonso Runzheimer, Katharina Jung, Sascha Rebrosova, Katarina Siler, Martin Moeller, Ralf Meyer, Vera Front Microbiol Microbiology Melanins are complex pigments with various biological functions and potential applications in space exploration and biomedicine due to their radioprotective properties. Aspergillus niger, a fungus known for its high radiation resistance, is widely used in biotechnology and a candidate for melanin production. In this study, we investigated the production of fungal pyomelanin (Pyo(Fun)) in A. niger by inducing overproduction of the pigment using L-tyrosine in a recombinant ΔhmgA mutant strain (OS4.3). The Pyo(Fun) pigment was characterized using three spectroscopic methods, and its antioxidant properties were assessed using a DPPH-assay. Additionally, we evaluated the protective effect of Pyo(Fun) against non-ionizing radiation (monochromatic UV-C) and compared its efficacy to a synthetically produced control pyomelanin (Pyo(Syn)). The results confirmed successful production of Pyo(Fun) in A. niger through inducible overproduction. Characterization using spectroscopic methods confirmed the presence of Pyo(Fun), and the DPPH-assay demonstrated its strong antioxidant properties. Moreover, Pyo(Fun) exhibited a highly protective effect against radiation-induced stress, surpassing the protection provided by Pyo(Syn). The findings of this study suggest that Pyo(Fun) has significant potential as a biological shield against harmful radiation. Notably, Pyo(Fun) is synthesized extracellularly, differing it from other fungal melanins (such as L-DOPA- or DHN-melanin) that require cell lysis for pigment purification. This characteristic makes Pyo(Fun) a valuable resource for biotechnology, biomedicine, and the space industry. However, further research is needed to evaluate its protective effect in a dried form and against ionizing radiation. Frontiers Media S.A. 2023-07-20 /pmc/articles/PMC10399693/ /pubmed/37547691 http://dx.doi.org/10.3389/fmicb.2023.1233740 Text en Copyright © 2023 Koch, Freidank-Pohl, Siontas, Cortesao, Mota, Runzheimer, Jung, Rebrosova, Siler, Moeller and Meyer. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Koch, Stella Marie
Freidank-Pohl, Carsten
Siontas, Oliver
Cortesao, Marta
Mota, Afonso
Runzheimer, Katharina
Jung, Sascha
Rebrosova, Katarina
Siler, Martin
Moeller, Ralf
Meyer, Vera
Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity
title Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity
title_full Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity
title_fullStr Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity
title_full_unstemmed Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity
title_short Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity
title_sort aspergillus niger as a cell factory for the production of pyomelanin, a molecule with uv-c radiation shielding activity
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10399693/
https://www.ncbi.nlm.nih.gov/pubmed/37547691
http://dx.doi.org/10.3389/fmicb.2023.1233740
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