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NKG2A and circulating extracellular vesicles are key regulators of natural killer cell activity in prostate cancer after prostatectomy
Extracellular vesicles (EVs) are an important regulatory factor for natural killer cell activity (NKA) in the tumor microenvironment. The relationship between circulating EVs in the peripheral blood and natural killer (NK) cells in prostate cancer (PCa) is unclear. This study aimed at investigating...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10399716/ https://www.ncbi.nlm.nih.gov/pubmed/36931723 http://dx.doi.org/10.1002/1878-0261.13422 |
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author | Lu, Yu‐Chuan Ho, Chen‐Hsun Hong, Jian‐Hua Kuo, Ming‐Chieh Liao, Yi‐An Jaw, Fu‐Shan Cheng, Jason Chia‐Hsien Huang, Chao‐Yuan Chang, Ko‐Ping Chen, Chung‐Hsin Lin, Jung‐An Hsiao, An Kung, Hsiu‐Ni |
author_facet | Lu, Yu‐Chuan Ho, Chen‐Hsun Hong, Jian‐Hua Kuo, Ming‐Chieh Liao, Yi‐An Jaw, Fu‐Shan Cheng, Jason Chia‐Hsien Huang, Chao‐Yuan Chang, Ko‐Ping Chen, Chung‐Hsin Lin, Jung‐An Hsiao, An Kung, Hsiu‐Ni |
author_sort | Lu, Yu‐Chuan |
collection | PubMed |
description | Extracellular vesicles (EVs) are an important regulatory factor for natural killer cell activity (NKA) in the tumor microenvironment. The relationship between circulating EVs in the peripheral blood and natural killer (NK) cells in prostate cancer (PCa) is unclear. This study aimed at investigating the key regulators in the interaction between circulating EVs and NK cells in PCa patients before and after tumor removal. NK‐cell characteristics were prospectively assessed in 79 patients treated with robot‐assisted laparoscopic radical prostatectomy preoperatively and postoperatively. Compared with healthy donors, the existence of prostate tumors increased the number of circulating EVs and altered ligand expression of EVs. Circulating EVs extracted from cancer patients significantly decreased NKA of NK cells compared with those extracted from healthy donors. Upon treatment with an inhibiting antibody or small interfering RNA, natural killer cell protein group 2A (NKG2A) was identified as the main NKA regulator in cancer patients for accepting the signal from circulating EVs. After surgery, NKA was increased and NKG2A expression on NK cells was significantly reduced. The expression of ligands for natural killer cell protein group 2D (NKG2D) on EVs and the level of circulation EVs both significantly increased. With the decrease in NKG2A levels on NK cells and the increase in total NKG2D ligands on circulating EVs, which was increased postoperatively, both NKG2A on NK cells and NKG2D ligands on circulating exosomes are main regulators of NKA restoration after prostatectomy. |
format | Online Article Text |
id | pubmed-10399716 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103997162023-08-04 NKG2A and circulating extracellular vesicles are key regulators of natural killer cell activity in prostate cancer after prostatectomy Lu, Yu‐Chuan Ho, Chen‐Hsun Hong, Jian‐Hua Kuo, Ming‐Chieh Liao, Yi‐An Jaw, Fu‐Shan Cheng, Jason Chia‐Hsien Huang, Chao‐Yuan Chang, Ko‐Ping Chen, Chung‐Hsin Lin, Jung‐An Hsiao, An Kung, Hsiu‐Ni Mol Oncol Research Articles Extracellular vesicles (EVs) are an important regulatory factor for natural killer cell activity (NKA) in the tumor microenvironment. The relationship between circulating EVs in the peripheral blood and natural killer (NK) cells in prostate cancer (PCa) is unclear. This study aimed at investigating the key regulators in the interaction between circulating EVs and NK cells in PCa patients before and after tumor removal. NK‐cell characteristics were prospectively assessed in 79 patients treated with robot‐assisted laparoscopic radical prostatectomy preoperatively and postoperatively. Compared with healthy donors, the existence of prostate tumors increased the number of circulating EVs and altered ligand expression of EVs. Circulating EVs extracted from cancer patients significantly decreased NKA of NK cells compared with those extracted from healthy donors. Upon treatment with an inhibiting antibody or small interfering RNA, natural killer cell protein group 2A (NKG2A) was identified as the main NKA regulator in cancer patients for accepting the signal from circulating EVs. After surgery, NKA was increased and NKG2A expression on NK cells was significantly reduced. The expression of ligands for natural killer cell protein group 2D (NKG2D) on EVs and the level of circulation EVs both significantly increased. With the decrease in NKG2A levels on NK cells and the increase in total NKG2D ligands on circulating EVs, which was increased postoperatively, both NKG2A on NK cells and NKG2D ligands on circulating exosomes are main regulators of NKA restoration after prostatectomy. John Wiley and Sons Inc. 2023-03-27 /pmc/articles/PMC10399716/ /pubmed/36931723 http://dx.doi.org/10.1002/1878-0261.13422 Text en © 2023 The Authors. Molecular Oncology published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Lu, Yu‐Chuan Ho, Chen‐Hsun Hong, Jian‐Hua Kuo, Ming‐Chieh Liao, Yi‐An Jaw, Fu‐Shan Cheng, Jason Chia‐Hsien Huang, Chao‐Yuan Chang, Ko‐Ping Chen, Chung‐Hsin Lin, Jung‐An Hsiao, An Kung, Hsiu‐Ni NKG2A and circulating extracellular vesicles are key regulators of natural killer cell activity in prostate cancer after prostatectomy |
title |
NKG2A and circulating extracellular vesicles are key regulators of natural killer cell activity in prostate cancer after prostatectomy |
title_full |
NKG2A and circulating extracellular vesicles are key regulators of natural killer cell activity in prostate cancer after prostatectomy |
title_fullStr |
NKG2A and circulating extracellular vesicles are key regulators of natural killer cell activity in prostate cancer after prostatectomy |
title_full_unstemmed |
NKG2A and circulating extracellular vesicles are key regulators of natural killer cell activity in prostate cancer after prostatectomy |
title_short |
NKG2A and circulating extracellular vesicles are key regulators of natural killer cell activity in prostate cancer after prostatectomy |
title_sort | nkg2a and circulating extracellular vesicles are key regulators of natural killer cell activity in prostate cancer after prostatectomy |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10399716/ https://www.ncbi.nlm.nih.gov/pubmed/36931723 http://dx.doi.org/10.1002/1878-0261.13422 |
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