Cargando…
Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway
The exosomes of mesenchymal stem cells have immunoregulatory properties and can effectively mitigate secondary neuroinflammation due to traumatic brain injury (TBI). In this study, we found that adipose-derived stem cell exosomes (ADSCs-Exo) could reduce the inflammatory response after traumatic bra...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams & Wilkins
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10399942/ https://www.ncbi.nlm.nih.gov/pubmed/37506308 http://dx.doi.org/10.1097/WNR.0000000000001941 |
_version_ | 1785084359786627072 |
---|---|
author | Tang, Linjun Xu, Yong Wang, Liangwei Pan, Jingjing |
author_facet | Tang, Linjun Xu, Yong Wang, Liangwei Pan, Jingjing |
author_sort | Tang, Linjun |
collection | PubMed |
description | The exosomes of mesenchymal stem cells have immunoregulatory properties and can effectively mitigate secondary neuroinflammation due to traumatic brain injury (TBI). In this study, we found that adipose-derived stem cell exosomes (ADSCs-Exo) could reduce the inflammatory response after traumatic brain injury by reducing NLRP3 inflammasome secretion by microglial. ADSCs-Exo were monitored by Western blot and electron microscopy. An in-vitro lipopolysaccharide (LPS)-caused primary microglia model and a TBI rat model were constructed. Functional recovery was examined using the modified neurological severity score and foot fault tests. Inflammasome inactivation in LPS-stimulated microglial, ADSCs-Exo can reduce the secretion of interleukin (IL)-1β, IL-6 and tumor necrosis factor α. Compared with PBS-processed controls, the sensorimotor functional recovery was significantly improved by exosome treatment after injury at 14–35 days. Additionally, NLRP3 inflammasome was stimulated within 24 h after TBI. ADSCs-Exo application led to remarkable down-expression of NLRP3 and caspase-1. ADSCs-Exo can ameliorate LPS-induced inflammatory activation by reducing microglial pro-inflammatory cytokines. Moreover, the neuroprotective effect of ADSCs-Exo may be partially attributed to the inhibition thereof on the formation of NLRP3-mediated inflammasome. Such findings imply a potential function of ADSCs-Exo in treating TBI. |
format | Online Article Text |
id | pubmed-10399942 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Lippincott Williams & Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-103999422023-08-04 Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway Tang, Linjun Xu, Yong Wang, Liangwei Pan, Jingjing Neuroreport Original Article The exosomes of mesenchymal stem cells have immunoregulatory properties and can effectively mitigate secondary neuroinflammation due to traumatic brain injury (TBI). In this study, we found that adipose-derived stem cell exosomes (ADSCs-Exo) could reduce the inflammatory response after traumatic brain injury by reducing NLRP3 inflammasome secretion by microglial. ADSCs-Exo were monitored by Western blot and electron microscopy. An in-vitro lipopolysaccharide (LPS)-caused primary microglia model and a TBI rat model were constructed. Functional recovery was examined using the modified neurological severity score and foot fault tests. Inflammasome inactivation in LPS-stimulated microglial, ADSCs-Exo can reduce the secretion of interleukin (IL)-1β, IL-6 and tumor necrosis factor α. Compared with PBS-processed controls, the sensorimotor functional recovery was significantly improved by exosome treatment after injury at 14–35 days. Additionally, NLRP3 inflammasome was stimulated within 24 h after TBI. ADSCs-Exo application led to remarkable down-expression of NLRP3 and caspase-1. ADSCs-Exo can ameliorate LPS-induced inflammatory activation by reducing microglial pro-inflammatory cytokines. Moreover, the neuroprotective effect of ADSCs-Exo may be partially attributed to the inhibition thereof on the formation of NLRP3-mediated inflammasome. Such findings imply a potential function of ADSCs-Exo in treating TBI. Lippincott Williams & Wilkins 2023-08-02 2023-07-23 /pmc/articles/PMC10399942/ /pubmed/37506308 http://dx.doi.org/10.1097/WNR.0000000000001941 Text en Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) , where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. |
spellingShingle | Original Article Tang, Linjun Xu, Yong Wang, Liangwei Pan, Jingjing Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway |
title | Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway |
title_full | Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway |
title_fullStr | Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway |
title_full_unstemmed | Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway |
title_short | Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway |
title_sort | adipose-derived stem cell exosomes ameliorate traumatic brain injury through the nlrp3 signaling pathway |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10399942/ https://www.ncbi.nlm.nih.gov/pubmed/37506308 http://dx.doi.org/10.1097/WNR.0000000000001941 |
work_keys_str_mv | AT tanglinjun adiposederivedstemcellexosomesamelioratetraumaticbraininjurythroughthenlrp3signalingpathway AT xuyong adiposederivedstemcellexosomesamelioratetraumaticbraininjurythroughthenlrp3signalingpathway AT wangliangwei adiposederivedstemcellexosomesamelioratetraumaticbraininjurythroughthenlrp3signalingpathway AT panjingjing adiposederivedstemcellexosomesamelioratetraumaticbraininjurythroughthenlrp3signalingpathway |