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Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway

The exosomes of mesenchymal stem cells have immunoregulatory properties and can effectively mitigate secondary neuroinflammation due to traumatic brain injury (TBI). In this study, we found that adipose-derived stem cell exosomes (ADSCs-Exo) could reduce the inflammatory response after traumatic bra...

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Autores principales: Tang, Linjun, Xu, Yong, Wang, Liangwei, Pan, Jingjing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10399942/
https://www.ncbi.nlm.nih.gov/pubmed/37506308
http://dx.doi.org/10.1097/WNR.0000000000001941
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author Tang, Linjun
Xu, Yong
Wang, Liangwei
Pan, Jingjing
author_facet Tang, Linjun
Xu, Yong
Wang, Liangwei
Pan, Jingjing
author_sort Tang, Linjun
collection PubMed
description The exosomes of mesenchymal stem cells have immunoregulatory properties and can effectively mitigate secondary neuroinflammation due to traumatic brain injury (TBI). In this study, we found that adipose-derived stem cell exosomes (ADSCs-Exo) could reduce the inflammatory response after traumatic brain injury by reducing NLRP3 inflammasome secretion by microglial. ADSCs-Exo were monitored by Western blot and electron microscopy. An in-vitro lipopolysaccharide (LPS)-caused primary microglia model and a TBI rat model were constructed. Functional recovery was examined using the modified neurological severity score and foot fault tests. Inflammasome inactivation in LPS-stimulated microglial, ADSCs-Exo can reduce the secretion of interleukin (IL)-1β, IL-6 and tumor necrosis factor α. Compared with PBS-processed controls, the sensorimotor functional recovery was significantly improved by exosome treatment after injury at 14–35 days. Additionally, NLRP3 inflammasome was stimulated within 24 h after TBI. ADSCs-Exo application led to remarkable down-expression of NLRP3 and caspase-1. ADSCs-Exo can ameliorate LPS-induced inflammatory activation by reducing microglial pro-inflammatory cytokines. Moreover, the neuroprotective effect of ADSCs-Exo may be partially attributed to the inhibition thereof on the formation of NLRP3-mediated inflammasome. Such findings imply a potential function of ADSCs-Exo in treating TBI.
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spelling pubmed-103999422023-08-04 Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway Tang, Linjun Xu, Yong Wang, Liangwei Pan, Jingjing Neuroreport Original Article The exosomes of mesenchymal stem cells have immunoregulatory properties and can effectively mitigate secondary neuroinflammation due to traumatic brain injury (TBI). In this study, we found that adipose-derived stem cell exosomes (ADSCs-Exo) could reduce the inflammatory response after traumatic brain injury by reducing NLRP3 inflammasome secretion by microglial. ADSCs-Exo were monitored by Western blot and electron microscopy. An in-vitro lipopolysaccharide (LPS)-caused primary microglia model and a TBI rat model were constructed. Functional recovery was examined using the modified neurological severity score and foot fault tests. Inflammasome inactivation in LPS-stimulated microglial, ADSCs-Exo can reduce the secretion of interleukin (IL)-1β, IL-6 and tumor necrosis factor α. Compared with PBS-processed controls, the sensorimotor functional recovery was significantly improved by exosome treatment after injury at 14–35 days. Additionally, NLRP3 inflammasome was stimulated within 24 h after TBI. ADSCs-Exo application led to remarkable down-expression of NLRP3 and caspase-1. ADSCs-Exo can ameliorate LPS-induced inflammatory activation by reducing microglial pro-inflammatory cytokines. Moreover, the neuroprotective effect of ADSCs-Exo may be partially attributed to the inhibition thereof on the formation of NLRP3-mediated inflammasome. Such findings imply a potential function of ADSCs-Exo in treating TBI. Lippincott Williams & Wilkins 2023-08-02 2023-07-23 /pmc/articles/PMC10399942/ /pubmed/37506308 http://dx.doi.org/10.1097/WNR.0000000000001941 Text en Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) , where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.
spellingShingle Original Article
Tang, Linjun
Xu, Yong
Wang, Liangwei
Pan, Jingjing
Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway
title Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway
title_full Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway
title_fullStr Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway
title_full_unstemmed Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway
title_short Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway
title_sort adipose-derived stem cell exosomes ameliorate traumatic brain injury through the nlrp3 signaling pathway
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10399942/
https://www.ncbi.nlm.nih.gov/pubmed/37506308
http://dx.doi.org/10.1097/WNR.0000000000001941
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