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Effect of vaginal microbiota on pregnancy outcomes of women from Northern China who conceived after IVF

OBJECTIVE: This study aimed to investigate the correlation between vaginal microbiota and pregnancy outcomes of women who achieved pregnancy via in vitro fertilization (IVF) in Northern China, and to determine a biomarker for evaluation of the risk of preterm births in these women. METHODS: In total...

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Autores principales: Tong, Yu, Sun, Qiang, Shao, Xiaoguang, Wang, Zhijian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400330/
https://www.ncbi.nlm.nih.gov/pubmed/37547323
http://dx.doi.org/10.3389/fendo.2023.1200002
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author Tong, Yu
Sun, Qiang
Shao, Xiaoguang
Wang, Zhijian
author_facet Tong, Yu
Sun, Qiang
Shao, Xiaoguang
Wang, Zhijian
author_sort Tong, Yu
collection PubMed
description OBJECTIVE: This study aimed to investigate the correlation between vaginal microbiota and pregnancy outcomes of women who achieved pregnancy via in vitro fertilization (IVF) in Northern China, and to determine a biomarker for evaluation of the risk of preterm births in these women. METHODS: In total, 19 women from Northern China women who conceived after IVF and 6 women who conceived naturally were recruited in this study. The vaginal samples of the healthy participants were collected throughout pregnancy, that is, during the first, second, and third trimesters. The V3–V4 region of 16S rRNA was used to analyze the vaginal microbiome, and the bioinformatic analysis was performed using QIIME Alpha and Beta diversity analysis. RESULTS: Either IVF group or Natural conception group, bacterial community diversities and total species number of vagnal samples from who delivered at term were significantly higher than those who delivered before term. Low abundance of vaginal bacteria indicates an increased risk of preterm delivery. Further, more abundant vaginal bacteria was found in first trimesters instead of the next two trimesters. Vignal samples collected during first trimester showed richer differences and more predictive value for pregnancy outcoes. In addition, the diversity of the vaginal bacterial community decreased as the gestational age increased, in all samples. Alloscardovia was only found in participants who conceived after IVF, and the percentage of Alloscardovia in viginal samples of normal delivery group is much higher than the samples from preterm delivery group.Vobrio specifically colonized in vagina of pregnant woman in AFT group (those who conceived after IVF (A), first trimester (F), and delivered at term (T)) and Sporosarcina was detected only in women with AFT and AST (those who conceived after IVF (A), second trimester (S), and delivered at term (T)). These data indicates that Alloscardovia, Vobrio and Sporosarcina have great potential in predicting pregnancy outcomes who pregnanted by vitro fertilization CONCLUSIONS: Vaginal microbiota were more stable in women who conceived naturally and those who carried pregnancy to term. Oceanobacillus might act as a positive biomarker, whereas Sulfurospirillum and Propionispira may act as negative biomarkers for the risk of preterm birth.
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spelling pubmed-104003302023-08-04 Effect of vaginal microbiota on pregnancy outcomes of women from Northern China who conceived after IVF Tong, Yu Sun, Qiang Shao, Xiaoguang Wang, Zhijian Front Endocrinol (Lausanne) Endocrinology OBJECTIVE: This study aimed to investigate the correlation between vaginal microbiota and pregnancy outcomes of women who achieved pregnancy via in vitro fertilization (IVF) in Northern China, and to determine a biomarker for evaluation of the risk of preterm births in these women. METHODS: In total, 19 women from Northern China women who conceived after IVF and 6 women who conceived naturally were recruited in this study. The vaginal samples of the healthy participants were collected throughout pregnancy, that is, during the first, second, and third trimesters. The V3–V4 region of 16S rRNA was used to analyze the vaginal microbiome, and the bioinformatic analysis was performed using QIIME Alpha and Beta diversity analysis. RESULTS: Either IVF group or Natural conception group, bacterial community diversities and total species number of vagnal samples from who delivered at term were significantly higher than those who delivered before term. Low abundance of vaginal bacteria indicates an increased risk of preterm delivery. Further, more abundant vaginal bacteria was found in first trimesters instead of the next two trimesters. Vignal samples collected during first trimester showed richer differences and more predictive value for pregnancy outcoes. In addition, the diversity of the vaginal bacterial community decreased as the gestational age increased, in all samples. Alloscardovia was only found in participants who conceived after IVF, and the percentage of Alloscardovia in viginal samples of normal delivery group is much higher than the samples from preterm delivery group.Vobrio specifically colonized in vagina of pregnant woman in AFT group (those who conceived after IVF (A), first trimester (F), and delivered at term (T)) and Sporosarcina was detected only in women with AFT and AST (those who conceived after IVF (A), second trimester (S), and delivered at term (T)). These data indicates that Alloscardovia, Vobrio and Sporosarcina have great potential in predicting pregnancy outcomes who pregnanted by vitro fertilization CONCLUSIONS: Vaginal microbiota were more stable in women who conceived naturally and those who carried pregnancy to term. Oceanobacillus might act as a positive biomarker, whereas Sulfurospirillum and Propionispira may act as negative biomarkers for the risk of preterm birth. Frontiers Media S.A. 2023-07-18 /pmc/articles/PMC10400330/ /pubmed/37547323 http://dx.doi.org/10.3389/fendo.2023.1200002 Text en Copyright © 2023 Tong, Sun, Shao and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Tong, Yu
Sun, Qiang
Shao, Xiaoguang
Wang, Zhijian
Effect of vaginal microbiota on pregnancy outcomes of women from Northern China who conceived after IVF
title Effect of vaginal microbiota on pregnancy outcomes of women from Northern China who conceived after IVF
title_full Effect of vaginal microbiota on pregnancy outcomes of women from Northern China who conceived after IVF
title_fullStr Effect of vaginal microbiota on pregnancy outcomes of women from Northern China who conceived after IVF
title_full_unstemmed Effect of vaginal microbiota on pregnancy outcomes of women from Northern China who conceived after IVF
title_short Effect of vaginal microbiota on pregnancy outcomes of women from Northern China who conceived after IVF
title_sort effect of vaginal microbiota on pregnancy outcomes of women from northern china who conceived after ivf
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400330/
https://www.ncbi.nlm.nih.gov/pubmed/37547323
http://dx.doi.org/10.3389/fendo.2023.1200002
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