Protocol for expression of murine milk using modified human breast pump parts

Understanding the nutritional and immunomodulatory components of breast milk is crucial to developing novel mechanisms to optimize neonatal health. Here, we present a protocol to express and isolate murine milk in sufficient quantities for further analysis of components and bioactivity. We describe...

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Detalles Bibliográficos
Autores principales: Meyer, Cydney, Alcorn, Joseph L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400948/
https://www.ncbi.nlm.nih.gov/pubmed/37516971
http://dx.doi.org/10.1016/j.xpro.2023.102475
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author Meyer, Cydney
Alcorn, Joseph L.
author_facet Meyer, Cydney
Alcorn, Joseph L.
author_sort Meyer, Cydney
collection PubMed
description Understanding the nutritional and immunomodulatory components of breast milk is crucial to developing novel mechanisms to optimize neonatal health. Here, we present a protocol to express and isolate murine milk in sufficient quantities for further analysis of components and bioactivity. We describe steps for separating dams from pups, administering intraperitoneal anesthetic and oxytocin, and expressing milk using a minimally modified and readily available commercial breast pump parts. For complete details on the use and execution of this protocol, please refer to Meyer et al. (2022).(1)
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spelling pubmed-104009482023-08-05 Protocol for expression of murine milk using modified human breast pump parts Meyer, Cydney Alcorn, Joseph L. STAR Protoc Protocol Understanding the nutritional and immunomodulatory components of breast milk is crucial to developing novel mechanisms to optimize neonatal health. Here, we present a protocol to express and isolate murine milk in sufficient quantities for further analysis of components and bioactivity. We describe steps for separating dams from pups, administering intraperitoneal anesthetic and oxytocin, and expressing milk using a minimally modified and readily available commercial breast pump parts. For complete details on the use and execution of this protocol, please refer to Meyer et al. (2022).(1) Elsevier 2023-07-29 /pmc/articles/PMC10400948/ /pubmed/37516971 http://dx.doi.org/10.1016/j.xpro.2023.102475 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Meyer, Cydney
Alcorn, Joseph L.
Protocol for expression of murine milk using modified human breast pump parts
title Protocol for expression of murine milk using modified human breast pump parts
title_full Protocol for expression of murine milk using modified human breast pump parts
title_fullStr Protocol for expression of murine milk using modified human breast pump parts
title_full_unstemmed Protocol for expression of murine milk using modified human breast pump parts
title_short Protocol for expression of murine milk using modified human breast pump parts
title_sort protocol for expression of murine milk using modified human breast pump parts
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400948/
https://www.ncbi.nlm.nih.gov/pubmed/37516971
http://dx.doi.org/10.1016/j.xpro.2023.102475
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