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Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates
Ralstonia solanacearum invades plants through their roots and causes devastating bacterial diseases in multiple crops. Here, we present a versatile inoculation assay in Arabidopsis thaliana seedlings grown in sterile agar plates. We describe steps for plant preparation, bacterial inoculation, tissue...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400951/ https://www.ncbi.nlm.nih.gov/pubmed/37515761 http://dx.doi.org/10.1016/j.xpro.2023.102474 |
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author | Yu, Gang Zhang, Lu Wang, Keke Macho, Alberto P. |
author_facet | Yu, Gang Zhang, Lu Wang, Keke Macho, Alberto P. |
author_sort | Yu, Gang |
collection | PubMed |
description | Ralstonia solanacearum invades plants through their roots and causes devastating bacterial diseases in multiple crops. Here, we present a versatile inoculation assay in Arabidopsis thaliana seedlings grown in sterile agar plates. We describe steps for plant preparation, bacterial inoculation, tissue sampling, and bacterial quantification. This protocol can be used for accurate assessment of bacterial colonization and observation of plant response to infection. For complete details on the use and execution of this protocol, please refer to Dindas et al. (2022).(1) |
format | Online Article Text |
id | pubmed-10400951 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-104009512023-08-05 Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates Yu, Gang Zhang, Lu Wang, Keke Macho, Alberto P. STAR Protoc Protocol Ralstonia solanacearum invades plants through their roots and causes devastating bacterial diseases in multiple crops. Here, we present a versatile inoculation assay in Arabidopsis thaliana seedlings grown in sterile agar plates. We describe steps for plant preparation, bacterial inoculation, tissue sampling, and bacterial quantification. This protocol can be used for accurate assessment of bacterial colonization and observation of plant response to infection. For complete details on the use and execution of this protocol, please refer to Dindas et al. (2022).(1) Elsevier 2023-07-28 /pmc/articles/PMC10400951/ /pubmed/37515761 http://dx.doi.org/10.1016/j.xpro.2023.102474 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Yu, Gang Zhang, Lu Wang, Keke Macho, Alberto P. Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates |
title | Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates |
title_full | Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates |
title_fullStr | Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates |
title_full_unstemmed | Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates |
title_short | Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates |
title_sort | inoculation of arabidopsis seedlings with ralstonia solanacearum in sterile agar plates |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400951/ https://www.ncbi.nlm.nih.gov/pubmed/37515761 http://dx.doi.org/10.1016/j.xpro.2023.102474 |
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