Two-photon manipulation of neuronal activity and behavior in Hydra vulgaris

The introduction of calcium imaging has rendered cnidarians, such as Hydra vulgaris, valuable model organisms for investigating neuronal activity and behavior. Here, we present a comprehensive protocol to image and manipulate neuronal activity and behavior of Hydra. We describe steps for wide-field...

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Detalles Bibliográficos
Autores principales: Yamamoto, Wataru, Yuste, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400962/
https://www.ncbi.nlm.nih.gov/pubmed/37515760
http://dx.doi.org/10.1016/j.xpro.2023.102453
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author Yamamoto, Wataru
Yuste, Rafael
author_facet Yamamoto, Wataru
Yuste, Rafael
author_sort Yamamoto, Wataru
collection PubMed
description The introduction of calcium imaging has rendered cnidarians, such as Hydra vulgaris, valuable model organisms for investigating neuronal activity and behavior. Here, we present a comprehensive protocol to image and manipulate neuronal activity and behavior of Hydra. We describe steps for wide-field imaging and two-photon simulation and ablation of neurons. We then detail imaging behavior and post-ablation analysis. We address challenges that may arise during the preparation and execution of the experiments.
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spelling pubmed-104009622023-08-05 Two-photon manipulation of neuronal activity and behavior in Hydra vulgaris Yamamoto, Wataru Yuste, Rafael STAR Protoc Protocol The introduction of calcium imaging has rendered cnidarians, such as Hydra vulgaris, valuable model organisms for investigating neuronal activity and behavior. Here, we present a comprehensive protocol to image and manipulate neuronal activity and behavior of Hydra. We describe steps for wide-field imaging and two-photon simulation and ablation of neurons. We then detail imaging behavior and post-ablation analysis. We address challenges that may arise during the preparation and execution of the experiments. Elsevier 2023-07-28 /pmc/articles/PMC10400962/ /pubmed/37515760 http://dx.doi.org/10.1016/j.xpro.2023.102453 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Yamamoto, Wataru
Yuste, Rafael
Two-photon manipulation of neuronal activity and behavior in Hydra vulgaris
title Two-photon manipulation of neuronal activity and behavior in Hydra vulgaris
title_full Two-photon manipulation of neuronal activity and behavior in Hydra vulgaris
title_fullStr Two-photon manipulation of neuronal activity and behavior in Hydra vulgaris
title_full_unstemmed Two-photon manipulation of neuronal activity and behavior in Hydra vulgaris
title_short Two-photon manipulation of neuronal activity and behavior in Hydra vulgaris
title_sort two-photon manipulation of neuronal activity and behavior in hydra vulgaris
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400962/
https://www.ncbi.nlm.nih.gov/pubmed/37515760
http://dx.doi.org/10.1016/j.xpro.2023.102453
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