3D biomimetic environment enabling ex utero trophoblast invasion and co-culture of embryos and somatic cells

The first direct contact between the embryo and the mother is established during implantation. This process is inaccessible for direct studies as the implanting embryo is concealed by the maternal tissues. Here, we present a protocol for establishing a 3D biomimetic environment based on synthetic hy...

Descripción completa

Detalles Bibliográficos
Autores principales: Govindasamy, Niraimathi, Long, Hongyan, Ranga, Adrian, Trappmann, Britta, Bedzhov, Ivan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400965/
https://www.ncbi.nlm.nih.gov/pubmed/37515766
http://dx.doi.org/10.1016/j.xpro.2023.102456
_version_ 1785084555988828160
author Govindasamy, Niraimathi
Long, Hongyan
Ranga, Adrian
Trappmann, Britta
Bedzhov, Ivan
author_facet Govindasamy, Niraimathi
Long, Hongyan
Ranga, Adrian
Trappmann, Britta
Bedzhov, Ivan
author_sort Govindasamy, Niraimathi
collection PubMed
description The first direct contact between the embryo and the mother is established during implantation. This process is inaccessible for direct studies as the implanting embryo is concealed by the maternal tissues. Here, we present a protocol for establishing a 3D biomimetic environment based on synthetic hydrogels which harbor key biomechanical properties of the uterine stroma. We describe steps for isolating and culturing embryos in PEG/DexMA hydrogel. We then detail the co-culture of embryos and endothelial cells in a microfluidic device. For complete details on the use and execution of this protocol, please refer to Govindasamy et al. (2021)(1) and Ozguldez et al. (2023).(2)
format Online
Article
Text
id pubmed-10400965
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-104009652023-08-05 3D biomimetic environment enabling ex utero trophoblast invasion and co-culture of embryos and somatic cells Govindasamy, Niraimathi Long, Hongyan Ranga, Adrian Trappmann, Britta Bedzhov, Ivan STAR Protoc Protocol The first direct contact between the embryo and the mother is established during implantation. This process is inaccessible for direct studies as the implanting embryo is concealed by the maternal tissues. Here, we present a protocol for establishing a 3D biomimetic environment based on synthetic hydrogels which harbor key biomechanical properties of the uterine stroma. We describe steps for isolating and culturing embryos in PEG/DexMA hydrogel. We then detail the co-culture of embryos and endothelial cells in a microfluidic device. For complete details on the use and execution of this protocol, please refer to Govindasamy et al. (2021)(1) and Ozguldez et al. (2023).(2) Elsevier 2023-07-28 /pmc/articles/PMC10400965/ /pubmed/37515766 http://dx.doi.org/10.1016/j.xpro.2023.102456 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Govindasamy, Niraimathi
Long, Hongyan
Ranga, Adrian
Trappmann, Britta
Bedzhov, Ivan
3D biomimetic environment enabling ex utero trophoblast invasion and co-culture of embryos and somatic cells
title 3D biomimetic environment enabling ex utero trophoblast invasion and co-culture of embryos and somatic cells
title_full 3D biomimetic environment enabling ex utero trophoblast invasion and co-culture of embryos and somatic cells
title_fullStr 3D biomimetic environment enabling ex utero trophoblast invasion and co-culture of embryos and somatic cells
title_full_unstemmed 3D biomimetic environment enabling ex utero trophoblast invasion and co-culture of embryos and somatic cells
title_short 3D biomimetic environment enabling ex utero trophoblast invasion and co-culture of embryos and somatic cells
title_sort 3d biomimetic environment enabling ex utero trophoblast invasion and co-culture of embryos and somatic cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10400965/
https://www.ncbi.nlm.nih.gov/pubmed/37515766
http://dx.doi.org/10.1016/j.xpro.2023.102456
work_keys_str_mv AT govindasamyniraimathi 3dbiomimeticenvironmentenablingexuterotrophoblastinvasionandcocultureofembryosandsomaticcells
AT longhongyan 3dbiomimeticenvironmentenablingexuterotrophoblastinvasionandcocultureofembryosandsomaticcells
AT rangaadrian 3dbiomimeticenvironmentenablingexuterotrophoblastinvasionandcocultureofembryosandsomaticcells
AT trappmannbritta 3dbiomimeticenvironmentenablingexuterotrophoblastinvasionandcocultureofembryosandsomaticcells
AT bedzhovivan 3dbiomimeticenvironmentenablingexuterotrophoblastinvasionandcocultureofembryosandsomaticcells

Ejemplares similares