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Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs

We first identified thrombomodulin (TM) and endothelial nitric oxide (NO) synthase as key factors for the antithrombogenic function of the endothelium in human atherosclerotic carotid arteries. Then, recombinant TM and an engineered galactosidase responsible for the conversion of an exogenous NO pro...

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Autores principales: Wei, Yongzhen, Jiang, Huan, Chai, Chao, Liu, Pei, Qian, Meng, Sun, Na, Gao, Man, Zu, Honglin, Yu, Yongquan, Ji, Guangbo, Zhang, Yating, Yang, Sen, He, Ju, Cheng, Jiansong, Tian, Jinwei, Zhao, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10401295/
https://www.ncbi.nlm.nih.gov/pubmed/37547067
http://dx.doi.org/10.1016/j.jacbts.2022.12.009
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author Wei, Yongzhen
Jiang, Huan
Chai, Chao
Liu, Pei
Qian, Meng
Sun, Na
Gao, Man
Zu, Honglin
Yu, Yongquan
Ji, Guangbo
Zhang, Yating
Yang, Sen
He, Ju
Cheng, Jiansong
Tian, Jinwei
Zhao, Qiang
author_facet Wei, Yongzhen
Jiang, Huan
Chai, Chao
Liu, Pei
Qian, Meng
Sun, Na
Gao, Man
Zu, Honglin
Yu, Yongquan
Ji, Guangbo
Zhang, Yating
Yang, Sen
He, Ju
Cheng, Jiansong
Tian, Jinwei
Zhao, Qiang
author_sort Wei, Yongzhen
collection PubMed
description We first identified thrombomodulin (TM) and endothelial nitric oxide (NO) synthase as key factors for the antithrombogenic function of the endothelium in human atherosclerotic carotid arteries. Then, recombinant TM and an engineered galactosidase responsible for the conversion of an exogenous NO prodrug were immobilized on the surface of the vascular grafts. Surface modification by TM and NO cooperatively enhanced the antithrombogenicity and patency of vascular grafts. Importantly, we found that the combination of TM and NO also promoted endothelialization, whereas it reduced adverse intimal hyperplasia, which is critical for the maintenance of vascular homeostasis, as confirmed in rat and pig models.
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spelling pubmed-104012952023-08-05 Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs Wei, Yongzhen Jiang, Huan Chai, Chao Liu, Pei Qian, Meng Sun, Na Gao, Man Zu, Honglin Yu, Yongquan Ji, Guangbo Zhang, Yating Yang, Sen He, Ju Cheng, Jiansong Tian, Jinwei Zhao, Qiang JACC Basic Transl Sci Original Research - Preclinical We first identified thrombomodulin (TM) and endothelial nitric oxide (NO) synthase as key factors for the antithrombogenic function of the endothelium in human atherosclerotic carotid arteries. Then, recombinant TM and an engineered galactosidase responsible for the conversion of an exogenous NO prodrug were immobilized on the surface of the vascular grafts. Surface modification by TM and NO cooperatively enhanced the antithrombogenicity and patency of vascular grafts. Importantly, we found that the combination of TM and NO also promoted endothelialization, whereas it reduced adverse intimal hyperplasia, which is critical for the maintenance of vascular homeostasis, as confirmed in rat and pig models. Elsevier 2023-05-03 /pmc/articles/PMC10401295/ /pubmed/37547067 http://dx.doi.org/10.1016/j.jacbts.2022.12.009 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research - Preclinical
Wei, Yongzhen
Jiang, Huan
Chai, Chao
Liu, Pei
Qian, Meng
Sun, Na
Gao, Man
Zu, Honglin
Yu, Yongquan
Ji, Guangbo
Zhang, Yating
Yang, Sen
He, Ju
Cheng, Jiansong
Tian, Jinwei
Zhao, Qiang
Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs
title Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs
title_full Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs
title_fullStr Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs
title_full_unstemmed Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs
title_short Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs
title_sort endothelium-mimetic surface modification improves antithrombogenicity and enhances patency of vascular grafts in rats and pigs
topic Original Research - Preclinical
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10401295/
https://www.ncbi.nlm.nih.gov/pubmed/37547067
http://dx.doi.org/10.1016/j.jacbts.2022.12.009
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