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Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs
We first identified thrombomodulin (TM) and endothelial nitric oxide (NO) synthase as key factors for the antithrombogenic function of the endothelium in human atherosclerotic carotid arteries. Then, recombinant TM and an engineered galactosidase responsible for the conversion of an exogenous NO pro...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10401295/ https://www.ncbi.nlm.nih.gov/pubmed/37547067 http://dx.doi.org/10.1016/j.jacbts.2022.12.009 |
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author | Wei, Yongzhen Jiang, Huan Chai, Chao Liu, Pei Qian, Meng Sun, Na Gao, Man Zu, Honglin Yu, Yongquan Ji, Guangbo Zhang, Yating Yang, Sen He, Ju Cheng, Jiansong Tian, Jinwei Zhao, Qiang |
author_facet | Wei, Yongzhen Jiang, Huan Chai, Chao Liu, Pei Qian, Meng Sun, Na Gao, Man Zu, Honglin Yu, Yongquan Ji, Guangbo Zhang, Yating Yang, Sen He, Ju Cheng, Jiansong Tian, Jinwei Zhao, Qiang |
author_sort | Wei, Yongzhen |
collection | PubMed |
description | We first identified thrombomodulin (TM) and endothelial nitric oxide (NO) synthase as key factors for the antithrombogenic function of the endothelium in human atherosclerotic carotid arteries. Then, recombinant TM and an engineered galactosidase responsible for the conversion of an exogenous NO prodrug were immobilized on the surface of the vascular grafts. Surface modification by TM and NO cooperatively enhanced the antithrombogenicity and patency of vascular grafts. Importantly, we found that the combination of TM and NO also promoted endothelialization, whereas it reduced adverse intimal hyperplasia, which is critical for the maintenance of vascular homeostasis, as confirmed in rat and pig models. |
format | Online Article Text |
id | pubmed-10401295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-104012952023-08-05 Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs Wei, Yongzhen Jiang, Huan Chai, Chao Liu, Pei Qian, Meng Sun, Na Gao, Man Zu, Honglin Yu, Yongquan Ji, Guangbo Zhang, Yating Yang, Sen He, Ju Cheng, Jiansong Tian, Jinwei Zhao, Qiang JACC Basic Transl Sci Original Research - Preclinical We first identified thrombomodulin (TM) and endothelial nitric oxide (NO) synthase as key factors for the antithrombogenic function of the endothelium in human atherosclerotic carotid arteries. Then, recombinant TM and an engineered galactosidase responsible for the conversion of an exogenous NO prodrug were immobilized on the surface of the vascular grafts. Surface modification by TM and NO cooperatively enhanced the antithrombogenicity and patency of vascular grafts. Importantly, we found that the combination of TM and NO also promoted endothelialization, whereas it reduced adverse intimal hyperplasia, which is critical for the maintenance of vascular homeostasis, as confirmed in rat and pig models. Elsevier 2023-05-03 /pmc/articles/PMC10401295/ /pubmed/37547067 http://dx.doi.org/10.1016/j.jacbts.2022.12.009 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Research - Preclinical Wei, Yongzhen Jiang, Huan Chai, Chao Liu, Pei Qian, Meng Sun, Na Gao, Man Zu, Honglin Yu, Yongquan Ji, Guangbo Zhang, Yating Yang, Sen He, Ju Cheng, Jiansong Tian, Jinwei Zhao, Qiang Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs |
title | Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs |
title_full | Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs |
title_fullStr | Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs |
title_full_unstemmed | Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs |
title_short | Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs |
title_sort | endothelium-mimetic surface modification improves antithrombogenicity and enhances patency of vascular grafts in rats and pigs |
topic | Original Research - Preclinical |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10401295/ https://www.ncbi.nlm.nih.gov/pubmed/37547067 http://dx.doi.org/10.1016/j.jacbts.2022.12.009 |
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