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Evaluating steroid hormone receptor interactions using the live-cell NanoBRET proximity assay
Steroid hormone receptors play a crucial role in the development and characterization of the majority of breast cancers. These receptors canonically function through homodimerization, but physical interactions between different hormone receptors play a key role in cell functions as well. The estroge...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10402027/ https://www.ncbi.nlm.nih.gov/pubmed/37546915 http://dx.doi.org/10.1101/2023.07.25.550078 |
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author | Huggins, Rosemary J Hosfield, David Ishag-Osman, Amira Lee, Keemin Ton-That, Elia Greene, Geoffrey L. |
author_facet | Huggins, Rosemary J Hosfield, David Ishag-Osman, Amira Lee, Keemin Ton-That, Elia Greene, Geoffrey L. |
author_sort | Huggins, Rosemary J |
collection | PubMed |
description | Steroid hormone receptors play a crucial role in the development and characterization of the majority of breast cancers. These receptors canonically function through homodimerization, but physical interactions between different hormone receptors play a key role in cell functions as well. The estrogen receptor (ERα) and progesterone receptor (PR), for example, are involved in a complex set of interactions known as ERα/PR crosstalk. Here, we developed a valuable panel of nuclear receptor expression plasmids specifically for use in NanoBRET assays to assess nuclear receptor homo- and heterodimerization. We demonstrate the utility of this assay system by assessing ERα/PR physical interaction in the context of the endocrine therapy resistance-associated ERα Y537S mutation. We identify a role of the ERα Y537S mutation beyond that of constitutive activity of the receptor; it also increases ERα/PR crosstalk. In total, the NanoBRET assay provides a novel avenue for investigating hormone receptor crosstalk. Future research may use this system to assess the effects of other clinically significant hormone receptor mutations on hormone receptor crosstalk. |
format | Online Article Text |
id | pubmed-10402027 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-104020272023-08-05 Evaluating steroid hormone receptor interactions using the live-cell NanoBRET proximity assay Huggins, Rosemary J Hosfield, David Ishag-Osman, Amira Lee, Keemin Ton-That, Elia Greene, Geoffrey L. bioRxiv Article Steroid hormone receptors play a crucial role in the development and characterization of the majority of breast cancers. These receptors canonically function through homodimerization, but physical interactions between different hormone receptors play a key role in cell functions as well. The estrogen receptor (ERα) and progesterone receptor (PR), for example, are involved in a complex set of interactions known as ERα/PR crosstalk. Here, we developed a valuable panel of nuclear receptor expression plasmids specifically for use in NanoBRET assays to assess nuclear receptor homo- and heterodimerization. We demonstrate the utility of this assay system by assessing ERα/PR physical interaction in the context of the endocrine therapy resistance-associated ERα Y537S mutation. We identify a role of the ERα Y537S mutation beyond that of constitutive activity of the receptor; it also increases ERα/PR crosstalk. In total, the NanoBRET assay provides a novel avenue for investigating hormone receptor crosstalk. Future research may use this system to assess the effects of other clinically significant hormone receptor mutations on hormone receptor crosstalk. Cold Spring Harbor Laboratory 2023-07-26 /pmc/articles/PMC10402027/ /pubmed/37546915 http://dx.doi.org/10.1101/2023.07.25.550078 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator. |
spellingShingle | Article Huggins, Rosemary J Hosfield, David Ishag-Osman, Amira Lee, Keemin Ton-That, Elia Greene, Geoffrey L. Evaluating steroid hormone receptor interactions using the live-cell NanoBRET proximity assay |
title | Evaluating steroid hormone receptor interactions using the live-cell NanoBRET proximity assay |
title_full | Evaluating steroid hormone receptor interactions using the live-cell NanoBRET proximity assay |
title_fullStr | Evaluating steroid hormone receptor interactions using the live-cell NanoBRET proximity assay |
title_full_unstemmed | Evaluating steroid hormone receptor interactions using the live-cell NanoBRET proximity assay |
title_short | Evaluating steroid hormone receptor interactions using the live-cell NanoBRET proximity assay |
title_sort | evaluating steroid hormone receptor interactions using the live-cell nanobret proximity assay |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10402027/ https://www.ncbi.nlm.nih.gov/pubmed/37546915 http://dx.doi.org/10.1101/2023.07.25.550078 |
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