Kinetic dissection of pre-crRNA binding and processing by CRISPR-Cas12a

CRISPR-Cas12a binds and processes a single pre-crRNA during maturation, providing a simple tool for genome editing applications. Here, we constructed a kinetic and thermodynamic framework for pre-crRNA processing by Cas12a in vitro, and we measured the contributions of distinct regions of the pre-cr...

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Detalles Bibliográficos
Autores principales: Sinan, Selma, Appleby, Nathan M., Russell, Rick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10402064/
https://www.ncbi.nlm.nih.gov/pubmed/37546762
http://dx.doi.org/10.1101/2023.07.25.550589

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10402064/
https://www.ncbi.nlm.nih.gov/pubmed/37546762
http://dx.doi.org/10.1101/2023.07.25.550589

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