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3D electronic implants in subretinal space: long-term follow-up in rodents
Photovoltaic subretinal prosthesis (PRIMA) enables restoration of sight via electrical stimulation of the interneurons in degenerated retina, with resolution limited by the 100 μm pixel size. Since decreasing the pixel size below 75 μm in the current bipolar geometry is impossible, we explore the po...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10402070/ https://www.ncbi.nlm.nih.gov/pubmed/37546971 http://dx.doi.org/10.1101/2023.07.25.550561 |
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author | Bhuckory, Mohajeet Wang, Bing-Yi Chen, Zhijie Charles Shin, Andrew Pham-Howard, Davis Shah, Sarthak Monkongpitukkul, Nicharee Galambos, Ludwig Kamins, Theodore Mathieson, Keith Palanker, Daniel |
author_facet | Bhuckory, Mohajeet Wang, Bing-Yi Chen, Zhijie Charles Shin, Andrew Pham-Howard, Davis Shah, Sarthak Monkongpitukkul, Nicharee Galambos, Ludwig Kamins, Theodore Mathieson, Keith Palanker, Daniel |
author_sort | Bhuckory, Mohajeet |
collection | PubMed |
description | Photovoltaic subretinal prosthesis (PRIMA) enables restoration of sight via electrical stimulation of the interneurons in degenerated retina, with resolution limited by the 100 μm pixel size. Since decreasing the pixel size below 75 μm in the current bipolar geometry is impossible, we explore the possibility of using smaller pixels based on a novel 3-dimensional honeycomb-shaped design. We assessed the long-term biocompatibility and stability of these arrays in rats by investigating the anatomical integration of the retina with flat and 3D implants and response to electrical stimulation over lifetime – up to 9 months post-implantation in aged rats. With both flat and 3D implants, VEP amplitude decreased after the day of implantation by more than 3-fold, and gradually recovered over about 3 months. With 25 µm high honeycomb walls, the majority of bipolar cells migrate into the wells, while amacrine and ganglion cells remain above the cavities, which is essential for selective network-mediated stimulation of the second-order neurons. Retinal thickness and full-field stimulation threshold with 40 µm-wide honeycomb pixels were comparable to those with planar devices – 0.05 mW/mm(2) with 10ms pulses. However, fewer cells from the inner nuclear layer migrated into the 20 µm-wide wells, and stimulation threshold increased over 5 months, before stabilizing at about 0.08 mW/mm(2). Such threshold is significantly lower than 1.8 mW/mm(2) with a previous design of flat bipolar pixels, confirming the promise of the 3D honeycomb-based approach to high resolution subretinal prosthesis. |
format | Online Article Text |
id | pubmed-10402070 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-104020702023-08-05 3D electronic implants in subretinal space: long-term follow-up in rodents Bhuckory, Mohajeet Wang, Bing-Yi Chen, Zhijie Charles Shin, Andrew Pham-Howard, Davis Shah, Sarthak Monkongpitukkul, Nicharee Galambos, Ludwig Kamins, Theodore Mathieson, Keith Palanker, Daniel bioRxiv Article Photovoltaic subretinal prosthesis (PRIMA) enables restoration of sight via electrical stimulation of the interneurons in degenerated retina, with resolution limited by the 100 μm pixel size. Since decreasing the pixel size below 75 μm in the current bipolar geometry is impossible, we explore the possibility of using smaller pixels based on a novel 3-dimensional honeycomb-shaped design. We assessed the long-term biocompatibility and stability of these arrays in rats by investigating the anatomical integration of the retina with flat and 3D implants and response to electrical stimulation over lifetime – up to 9 months post-implantation in aged rats. With both flat and 3D implants, VEP amplitude decreased after the day of implantation by more than 3-fold, and gradually recovered over about 3 months. With 25 µm high honeycomb walls, the majority of bipolar cells migrate into the wells, while amacrine and ganglion cells remain above the cavities, which is essential for selective network-mediated stimulation of the second-order neurons. Retinal thickness and full-field stimulation threshold with 40 µm-wide honeycomb pixels were comparable to those with planar devices – 0.05 mW/mm(2) with 10ms pulses. However, fewer cells from the inner nuclear layer migrated into the 20 µm-wide wells, and stimulation threshold increased over 5 months, before stabilizing at about 0.08 mW/mm(2). Such threshold is significantly lower than 1.8 mW/mm(2) with a previous design of flat bipolar pixels, confirming the promise of the 3D honeycomb-based approach to high resolution subretinal prosthesis. Cold Spring Harbor Laboratory 2023-07-28 /pmc/articles/PMC10402070/ /pubmed/37546971 http://dx.doi.org/10.1101/2023.07.25.550561 Text en https://creativecommons.org/licenses/by-nd/4.0/This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, and only so long as attribution is given to the creator. The license allows for commercial use. |
spellingShingle | Article Bhuckory, Mohajeet Wang, Bing-Yi Chen, Zhijie Charles Shin, Andrew Pham-Howard, Davis Shah, Sarthak Monkongpitukkul, Nicharee Galambos, Ludwig Kamins, Theodore Mathieson, Keith Palanker, Daniel 3D electronic implants in subretinal space: long-term follow-up in rodents |
title | 3D electronic implants in subretinal space: long-term follow-up in rodents |
title_full | 3D electronic implants in subretinal space: long-term follow-up in rodents |
title_fullStr | 3D electronic implants in subretinal space: long-term follow-up in rodents |
title_full_unstemmed | 3D electronic implants in subretinal space: long-term follow-up in rodents |
title_short | 3D electronic implants in subretinal space: long-term follow-up in rodents |
title_sort | 3d electronic implants in subretinal space: long-term follow-up in rodents |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10402070/ https://www.ncbi.nlm.nih.gov/pubmed/37546971 http://dx.doi.org/10.1101/2023.07.25.550561 |
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