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An Evaluation of Antibacterial Effects of Human Amniotic Fluid on Pathogenic and Probiotic Bacteria In Vitro

BACKGROUND: Amniotic fluid in the uterus is beneficial for the fetus growth and protection due to its nutritional elements as well as its antibacterial and anti-inflammatory properties. Today, body membranes are increasingly being used in multiple fields. The purpose of the current study was evaluat...

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Autores principales: Ghanbarzadeh, Nahid, Mohammadparast-Tabas, Pouria, Aramjoo, Hamed, Allahyari, Elahe, Ghasemi, Saeedeh, Erfani, Soheila, Mesbahzadeh, Behzad, Dehghan, Hamideh, Zare-Bidaki, Majid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2023
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10402456/
https://www.ncbi.nlm.nih.gov/pubmed/37547569
http://dx.doi.org/10.18502/jri.v24i2.12495
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author Ghanbarzadeh, Nahid
Mohammadparast-Tabas, Pouria
Aramjoo, Hamed
Allahyari, Elahe
Ghasemi, Saeedeh
Erfani, Soheila
Mesbahzadeh, Behzad
Dehghan, Hamideh
Zare-Bidaki, Majid
author_facet Ghanbarzadeh, Nahid
Mohammadparast-Tabas, Pouria
Aramjoo, Hamed
Allahyari, Elahe
Ghasemi, Saeedeh
Erfani, Soheila
Mesbahzadeh, Behzad
Dehghan, Hamideh
Zare-Bidaki, Majid
author_sort Ghanbarzadeh, Nahid
collection PubMed
description BACKGROUND: Amniotic fluid in the uterus is beneficial for the fetus growth and protection due to its nutritional elements as well as its antibacterial and anti-inflammatory properties. Today, body membranes are increasingly being used in multiple fields. The purpose of the current study was evaluation of the antibacterial effects of amniotic fluid and comparison of its effects on pathogenic and probiotic bacteria. METHODS: This experimental study was conducted on amniotic fluid obtained from 43 healthy mothers who gave birth by selective cesarean section. Then, antibacterial effects of amniotic fluids were investigated on 8 standard bacterial strains, including Bacillus cereus, Escherichia coli, Staphylococcus aureus, Shigella flexneri, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus cereus, and Lactobacillus plantarum by agar well-diffusion method. Data analysis was performed by SPSS software, vs. 22 (IBM, US). RESULTS: Amniotic fluid revealed an inhibitory effect on the growth of bacterial strains. Staphylococcus aureus and Streptococcus pyogenes strains showed growth inhibition in 39% and 17% of samples, respectively. In other bacterial strains, there was growth inhibition in less than 5% of the samples. Also, the zone of growth inhibition for Staphylococcus aureus and Streptococcus pyogenes was significantly higher than the other strains. Amniotic fluid samples had an antibacterial effect on all pathogen strains in general, but not on the Lactobacillus plantarum probiotic strain. CONCLUSION: Our findings suggest that the antibacterial effect of amniotic fluid on pathogenic bacteria is significantly higher than the Lactobacillus plantarum as a probiotic one. Overall, the findings support the use of natural substances as alternative therapeutic agents to combat antibiotic resistance.
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spelling pubmed-104024562023-08-05 An Evaluation of Antibacterial Effects of Human Amniotic Fluid on Pathogenic and Probiotic Bacteria In Vitro Ghanbarzadeh, Nahid Mohammadparast-Tabas, Pouria Aramjoo, Hamed Allahyari, Elahe Ghasemi, Saeedeh Erfani, Soheila Mesbahzadeh, Behzad Dehghan, Hamideh Zare-Bidaki, Majid J Reprod Infertil Original Article BACKGROUND: Amniotic fluid in the uterus is beneficial for the fetus growth and protection due to its nutritional elements as well as its antibacterial and anti-inflammatory properties. Today, body membranes are increasingly being used in multiple fields. The purpose of the current study was evaluation of the antibacterial effects of amniotic fluid and comparison of its effects on pathogenic and probiotic bacteria. METHODS: This experimental study was conducted on amniotic fluid obtained from 43 healthy mothers who gave birth by selective cesarean section. Then, antibacterial effects of amniotic fluids were investigated on 8 standard bacterial strains, including Bacillus cereus, Escherichia coli, Staphylococcus aureus, Shigella flexneri, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus cereus, and Lactobacillus plantarum by agar well-diffusion method. Data analysis was performed by SPSS software, vs. 22 (IBM, US). RESULTS: Amniotic fluid revealed an inhibitory effect on the growth of bacterial strains. Staphylococcus aureus and Streptococcus pyogenes strains showed growth inhibition in 39% and 17% of samples, respectively. In other bacterial strains, there was growth inhibition in less than 5% of the samples. Also, the zone of growth inhibition for Staphylococcus aureus and Streptococcus pyogenes was significantly higher than the other strains. Amniotic fluid samples had an antibacterial effect on all pathogen strains in general, but not on the Lactobacillus plantarum probiotic strain. CONCLUSION: Our findings suggest that the antibacterial effect of amniotic fluid on pathogenic bacteria is significantly higher than the Lactobacillus plantarum as a probiotic one. Overall, the findings support the use of natural substances as alternative therapeutic agents to combat antibiotic resistance. Avicenna Research Institute 2023 /pmc/articles/PMC10402456/ /pubmed/37547569 http://dx.doi.org/10.18502/jri.v24i2.12495 Text en Copyright© 2023, Avicenna Research Institute. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/)
spellingShingle Original Article
Ghanbarzadeh, Nahid
Mohammadparast-Tabas, Pouria
Aramjoo, Hamed
Allahyari, Elahe
Ghasemi, Saeedeh
Erfani, Soheila
Mesbahzadeh, Behzad
Dehghan, Hamideh
Zare-Bidaki, Majid
An Evaluation of Antibacterial Effects of Human Amniotic Fluid on Pathogenic and Probiotic Bacteria In Vitro
title An Evaluation of Antibacterial Effects of Human Amniotic Fluid on Pathogenic and Probiotic Bacteria In Vitro
title_full An Evaluation of Antibacterial Effects of Human Amniotic Fluid on Pathogenic and Probiotic Bacteria In Vitro
title_fullStr An Evaluation of Antibacterial Effects of Human Amniotic Fluid on Pathogenic and Probiotic Bacteria In Vitro
title_full_unstemmed An Evaluation of Antibacterial Effects of Human Amniotic Fluid on Pathogenic and Probiotic Bacteria In Vitro
title_short An Evaluation of Antibacterial Effects of Human Amniotic Fluid on Pathogenic and Probiotic Bacteria In Vitro
title_sort evaluation of antibacterial effects of human amniotic fluid on pathogenic and probiotic bacteria in vitro
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10402456/
https://www.ncbi.nlm.nih.gov/pubmed/37547569
http://dx.doi.org/10.18502/jri.v24i2.12495
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