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mmu-miRNA-342-3p promotes hepatic stellate cell activation and hepatic fibrosis induced by Echinococcus multilocularis infection via targeting Zbtb7a
Liver fibrosis is one of the histopathological characters during Echinococcus multilocularis infection. The activation of hepatic stellate cells (HSCs) is a key event in the development of liver fibrosis. However, the molecular mechanism of HSC activation in the E. multilocularis infection-induced l...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10403128/ https://www.ncbi.nlm.nih.gov/pubmed/37490505 http://dx.doi.org/10.1371/journal.pntd.0011520 |
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author | Cao, Shanling Wang, Dexian Wu, Yixuan Zhang, Junmei Pu, Lixia Luo, Xuenong Zhang, Xueyong Sun, Xiaolin Zheng, Yadong Wang, Shuai Guo, Xiaola |
author_facet | Cao, Shanling Wang, Dexian Wu, Yixuan Zhang, Junmei Pu, Lixia Luo, Xuenong Zhang, Xueyong Sun, Xiaolin Zheng, Yadong Wang, Shuai Guo, Xiaola |
author_sort | Cao, Shanling |
collection | PubMed |
description | Liver fibrosis is one of the histopathological characters during Echinococcus multilocularis infection. The activation of hepatic stellate cells (HSCs) is a key event in the development of liver fibrosis. However, the molecular mechanism of HSC activation in the E. multilocularis infection-induced liver fibrosis remains largely unclear. Here, we reported that mmu-miR-342-3p was most dominantly expressed in HSCs and was upregulated in the HSCs in response to E. multilocularis infection. We further showed that mmu-miR-342-3p was able to bind to the 3’ UTR of the Zbtb7a gene and regulated its expression. Moreover, mmu-miR-342-3p expression was negatively correlated with its target gene Zbtb7a in HSCs during E. multilocularis infection. Knockdown of mmu-miR-342-3p promoted the expression of Gfap in the activated HSCs in vitro. In the E. multilocularis-infected mice, knockdown of mmu-miR-342-3p suppressed the expression of α-Sma, Col1α1, and TGF-β but promoted the expression of Gfap. Therefore, mmu-miR-342-3p is a key regulator for activation of HSCs, and inhibiting mmu-miR-342-3p to suppressed Zbtb7a-mediated TGF-β signaling in activated HSCs could be a novel strategy to treat liver fibrosis induced by E. multilocularis. |
format | Online Article Text |
id | pubmed-10403128 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-104031282023-08-05 mmu-miRNA-342-3p promotes hepatic stellate cell activation and hepatic fibrosis induced by Echinococcus multilocularis infection via targeting Zbtb7a Cao, Shanling Wang, Dexian Wu, Yixuan Zhang, Junmei Pu, Lixia Luo, Xuenong Zhang, Xueyong Sun, Xiaolin Zheng, Yadong Wang, Shuai Guo, Xiaola PLoS Negl Trop Dis Research Article Liver fibrosis is one of the histopathological characters during Echinococcus multilocularis infection. The activation of hepatic stellate cells (HSCs) is a key event in the development of liver fibrosis. However, the molecular mechanism of HSC activation in the E. multilocularis infection-induced liver fibrosis remains largely unclear. Here, we reported that mmu-miR-342-3p was most dominantly expressed in HSCs and was upregulated in the HSCs in response to E. multilocularis infection. We further showed that mmu-miR-342-3p was able to bind to the 3’ UTR of the Zbtb7a gene and regulated its expression. Moreover, mmu-miR-342-3p expression was negatively correlated with its target gene Zbtb7a in HSCs during E. multilocularis infection. Knockdown of mmu-miR-342-3p promoted the expression of Gfap in the activated HSCs in vitro. In the E. multilocularis-infected mice, knockdown of mmu-miR-342-3p suppressed the expression of α-Sma, Col1α1, and TGF-β but promoted the expression of Gfap. Therefore, mmu-miR-342-3p is a key regulator for activation of HSCs, and inhibiting mmu-miR-342-3p to suppressed Zbtb7a-mediated TGF-β signaling in activated HSCs could be a novel strategy to treat liver fibrosis induced by E. multilocularis. Public Library of Science 2023-07-25 /pmc/articles/PMC10403128/ /pubmed/37490505 http://dx.doi.org/10.1371/journal.pntd.0011520 Text en © 2023 Cao et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Cao, Shanling Wang, Dexian Wu, Yixuan Zhang, Junmei Pu, Lixia Luo, Xuenong Zhang, Xueyong Sun, Xiaolin Zheng, Yadong Wang, Shuai Guo, Xiaola mmu-miRNA-342-3p promotes hepatic stellate cell activation and hepatic fibrosis induced by Echinococcus multilocularis infection via targeting Zbtb7a |
title | mmu-miRNA-342-3p promotes hepatic stellate cell activation and hepatic fibrosis induced by Echinococcus multilocularis infection via targeting Zbtb7a |
title_full | mmu-miRNA-342-3p promotes hepatic stellate cell activation and hepatic fibrosis induced by Echinococcus multilocularis infection via targeting Zbtb7a |
title_fullStr | mmu-miRNA-342-3p promotes hepatic stellate cell activation and hepatic fibrosis induced by Echinococcus multilocularis infection via targeting Zbtb7a |
title_full_unstemmed | mmu-miRNA-342-3p promotes hepatic stellate cell activation and hepatic fibrosis induced by Echinococcus multilocularis infection via targeting Zbtb7a |
title_short | mmu-miRNA-342-3p promotes hepatic stellate cell activation and hepatic fibrosis induced by Echinococcus multilocularis infection via targeting Zbtb7a |
title_sort | mmu-mirna-342-3p promotes hepatic stellate cell activation and hepatic fibrosis induced by echinococcus multilocularis infection via targeting zbtb7a |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10403128/ https://www.ncbi.nlm.nih.gov/pubmed/37490505 http://dx.doi.org/10.1371/journal.pntd.0011520 |
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