Cargando…
Protocol for Biospecimen Collection and Analysis Within the BACPAC Research Program
The Biospecimen Collection and Processing Working Group of the National Institutes of Health (NIH) HEAL Initiative BACPAC Research Program was charged with identifying molecular biomarkers of interest to chronic low back pain (cLBP). Having identified biomarkers of interest, the Working Group worked...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10403310/ https://www.ncbi.nlm.nih.gov/pubmed/36525387 http://dx.doi.org/10.1093/pm/pnac197 |
_version_ | 1785085038175453184 |
---|---|
author | Fields, Aaron J Dudli, Stefan Schrepf, Andrew Kim, Angie Pham, Bernice Gallego, Estefania Mendoza, Sandra Meropol, Sharon B Darwin, Jessa Sowa, Gwendolyn Vo, Nam V |
author_facet | Fields, Aaron J Dudli, Stefan Schrepf, Andrew Kim, Angie Pham, Bernice Gallego, Estefania Mendoza, Sandra Meropol, Sharon B Darwin, Jessa Sowa, Gwendolyn Vo, Nam V |
author_sort | Fields, Aaron J |
collection | PubMed |
description | The Biospecimen Collection and Processing Working Group of the National Institutes of Health (NIH) HEAL Initiative BACPAC Research Program was charged with identifying molecular biomarkers of interest to chronic low back pain (cLBP). Having identified biomarkers of interest, the Working Group worked with the New York University Grossman School of Medicine, Center for Biospecimen Research and Development—funded by the Early Phase Pain Investigation Clinical Network Data Coordinating Center—to harmonize consortium-wide and site-specific efforts for biospecimen collection and analysis. Biospecimen collected are saliva, blood (whole, plasma, serum), urine, stool, and spine tissue (paraspinal muscle, ligamentum flavum, vertebral bone, facet cartilage, disc endplate, annulus fibrosus, or nucleus pulposus). The omics data acquisition and analyses derived from the biospecimen include genomics and epigenetics from DNA, proteomics from protein, transcriptomics from RNA, and microbiomics from 16S rRNA. These analyses contribute to the overarching goal of BACPAC to phenotype cLBP and will guide future efforts for precision medicine treatment. |
format | Online Article Text |
id | pubmed-10403310 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-104033102023-08-05 Protocol for Biospecimen Collection and Analysis Within the BACPAC Research Program Fields, Aaron J Dudli, Stefan Schrepf, Andrew Kim, Angie Pham, Bernice Gallego, Estefania Mendoza, Sandra Meropol, Sharon B Darwin, Jessa Sowa, Gwendolyn Vo, Nam V Pain Med Original Research Article The Biospecimen Collection and Processing Working Group of the National Institutes of Health (NIH) HEAL Initiative BACPAC Research Program was charged with identifying molecular biomarkers of interest to chronic low back pain (cLBP). Having identified biomarkers of interest, the Working Group worked with the New York University Grossman School of Medicine, Center for Biospecimen Research and Development—funded by the Early Phase Pain Investigation Clinical Network Data Coordinating Center—to harmonize consortium-wide and site-specific efforts for biospecimen collection and analysis. Biospecimen collected are saliva, blood (whole, plasma, serum), urine, stool, and spine tissue (paraspinal muscle, ligamentum flavum, vertebral bone, facet cartilage, disc endplate, annulus fibrosus, or nucleus pulposus). The omics data acquisition and analyses derived from the biospecimen include genomics and epigenetics from DNA, proteomics from protein, transcriptomics from RNA, and microbiomics from 16S rRNA. These analyses contribute to the overarching goal of BACPAC to phenotype cLBP and will guide future efforts for precision medicine treatment. Oxford University Press 2022-12-16 /pmc/articles/PMC10403310/ /pubmed/36525387 http://dx.doi.org/10.1093/pm/pnac197 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of the American Academy of Pain Medicine. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Article Fields, Aaron J Dudli, Stefan Schrepf, Andrew Kim, Angie Pham, Bernice Gallego, Estefania Mendoza, Sandra Meropol, Sharon B Darwin, Jessa Sowa, Gwendolyn Vo, Nam V Protocol for Biospecimen Collection and Analysis Within the BACPAC Research Program |
title | Protocol for Biospecimen Collection and Analysis Within the BACPAC Research Program |
title_full | Protocol for Biospecimen Collection and Analysis Within the BACPAC Research Program |
title_fullStr | Protocol for Biospecimen Collection and Analysis Within the BACPAC Research Program |
title_full_unstemmed | Protocol for Biospecimen Collection and Analysis Within the BACPAC Research Program |
title_short | Protocol for Biospecimen Collection and Analysis Within the BACPAC Research Program |
title_sort | protocol for biospecimen collection and analysis within the bacpac research program |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10403310/ https://www.ncbi.nlm.nih.gov/pubmed/36525387 http://dx.doi.org/10.1093/pm/pnac197 |
work_keys_str_mv | AT fieldsaaronj protocolforbiospecimencollectionandanalysiswithinthebacpacresearchprogram AT dudlistefan protocolforbiospecimencollectionandanalysiswithinthebacpacresearchprogram AT schrepfandrew protocolforbiospecimencollectionandanalysiswithinthebacpacresearchprogram AT kimangie protocolforbiospecimencollectionandanalysiswithinthebacpacresearchprogram AT phambernice protocolforbiospecimencollectionandanalysiswithinthebacpacresearchprogram AT gallegoestefania protocolforbiospecimencollectionandanalysiswithinthebacpacresearchprogram AT mendozasandra protocolforbiospecimencollectionandanalysiswithinthebacpacresearchprogram AT meropolsharonb protocolforbiospecimencollectionandanalysiswithinthebacpacresearchprogram AT darwinjessa protocolforbiospecimencollectionandanalysiswithinthebacpacresearchprogram AT sowagwendolyn protocolforbiospecimencollectionandanalysiswithinthebacpacresearchprogram AT vonamv protocolforbiospecimencollectionandanalysiswithinthebacpacresearchprogram |