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Dehydrocostus lactone inhibits Candida albicans growth and biofilm formation
Candida albicans infections are threatening public health but there are only several antifungal drugs available. This study was to assess the effects of dehydrocostus lactone (DL) on the Candida albicans growth and biofilms Microdilution assays revealed that DL inhibits a panel of standard Candida s...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10403490/ https://www.ncbi.nlm.nih.gov/pubmed/37540386 http://dx.doi.org/10.1186/s13568-023-01587-y |
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author | Zhang, Jingxiao Sun, Jian Zhang, Yu Zhang, Min Liu, Xin Yang, Longfei Yin, Yongjie |
author_facet | Zhang, Jingxiao Sun, Jian Zhang, Yu Zhang, Min Liu, Xin Yang, Longfei Yin, Yongjie |
author_sort | Zhang, Jingxiao |
collection | PubMed |
description | Candida albicans infections are threatening public health but there are only several antifungal drugs available. This study was to assess the effects of dehydrocostus lactone (DL) on the Candida albicans growth and biofilms Microdilution assays revealed that DL inhibits a panel of standard Candida species, including C. albicans, as well as 9 C. albicans clinical isolates. The morphological transition of C. albicans in RPMI-1640 medium and the adhesion to polystyrene surfaces can also be decreased by DL treatment, as evidenced by microscopic, metabolic activity and colony forming unit (CFU) counting assays. The XTT assay and microscopy inspection demonstrated that DL can inhibit the biofilms of C. albicans. Confocal microscopy following propidium iodide (PI) staining and DCFH-DA staining after DL treatment revealed that DL can increase the membrane permeability and intracellular reactive oxygen species (ROS) production. N-acetyl-cysteine could mitigate the inhibitory effects of DL on growth, morphological transition and biofilm formation, further confirming that ROS production induced by DL contributes to its antifungal and antibiofilm effects. This study showed that DL demonstrated antifungal and antibiofilm activity against C. albicans. The antifungal mechanisms may involve membrane damage and ROS overproduction. This study shows the potential of DL to fight Candida infections. |
format | Online Article Text |
id | pubmed-10403490 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-104034902023-08-06 Dehydrocostus lactone inhibits Candida albicans growth and biofilm formation Zhang, Jingxiao Sun, Jian Zhang, Yu Zhang, Min Liu, Xin Yang, Longfei Yin, Yongjie AMB Express Original Article Candida albicans infections are threatening public health but there are only several antifungal drugs available. This study was to assess the effects of dehydrocostus lactone (DL) on the Candida albicans growth and biofilms Microdilution assays revealed that DL inhibits a panel of standard Candida species, including C. albicans, as well as 9 C. albicans clinical isolates. The morphological transition of C. albicans in RPMI-1640 medium and the adhesion to polystyrene surfaces can also be decreased by DL treatment, as evidenced by microscopic, metabolic activity and colony forming unit (CFU) counting assays. The XTT assay and microscopy inspection demonstrated that DL can inhibit the biofilms of C. albicans. Confocal microscopy following propidium iodide (PI) staining and DCFH-DA staining after DL treatment revealed that DL can increase the membrane permeability and intracellular reactive oxygen species (ROS) production. N-acetyl-cysteine could mitigate the inhibitory effects of DL on growth, morphological transition and biofilm formation, further confirming that ROS production induced by DL contributes to its antifungal and antibiofilm effects. This study showed that DL demonstrated antifungal and antibiofilm activity against C. albicans. The antifungal mechanisms may involve membrane damage and ROS overproduction. This study shows the potential of DL to fight Candida infections. Springer Berlin Heidelberg 2023-08-04 /pmc/articles/PMC10403490/ /pubmed/37540386 http://dx.doi.org/10.1186/s13568-023-01587-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Zhang, Jingxiao Sun, Jian Zhang, Yu Zhang, Min Liu, Xin Yang, Longfei Yin, Yongjie Dehydrocostus lactone inhibits Candida albicans growth and biofilm formation |
title | Dehydrocostus lactone inhibits Candida albicans growth and biofilm formation |
title_full | Dehydrocostus lactone inhibits Candida albicans growth and biofilm formation |
title_fullStr | Dehydrocostus lactone inhibits Candida albicans growth and biofilm formation |
title_full_unstemmed | Dehydrocostus lactone inhibits Candida albicans growth and biofilm formation |
title_short | Dehydrocostus lactone inhibits Candida albicans growth and biofilm formation |
title_sort | dehydrocostus lactone inhibits candida albicans growth and biofilm formation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10403490/ https://www.ncbi.nlm.nih.gov/pubmed/37540386 http://dx.doi.org/10.1186/s13568-023-01587-y |
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