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Antibodies against native proteins of Mycobacterium tuberculosis can detect pulmonary tuberculosis patients

Accurate point-of-care testing (POCT) is critical for managing tuberculosis (TB). However, current antibody-based diagnosis shows low specificity and sensitivity. To find proper antigen candidates for TB diagnosis by antibodies, we assessed IgGs responsiveness to Mycobacterium tuberculosis proteins...

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Autores principales: Dewi, Desak Nyoman Surya Suameitria, Mertaniasih, Ni Made, Soedarsono, Hagino, Kimika, Yamazaki, Tomoya, Ozeki, Yuriko, Artama, Wayan Tunas, Kobayashi, Haruka, Inouchi, Erina, Yoshida, Yutaka, Ishikawa, Satoshi, Shaban, Amina Kaboso, Tateishi, Yoshitaka, Nishiyama, Akihito, Ato, Manabu, Matsumoto, Sohkichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10403504/
https://www.ncbi.nlm.nih.gov/pubmed/37542102
http://dx.doi.org/10.1038/s41598-023-39436-4
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author Dewi, Desak Nyoman Surya Suameitria
Mertaniasih, Ni Made
Soedarsono
Hagino, Kimika
Yamazaki, Tomoya
Ozeki, Yuriko
Artama, Wayan Tunas
Kobayashi, Haruka
Inouchi, Erina
Yoshida, Yutaka
Ishikawa, Satoshi
Shaban, Amina Kaboso
Tateishi, Yoshitaka
Nishiyama, Akihito
Ato, Manabu
Matsumoto, Sohkichi
author_facet Dewi, Desak Nyoman Surya Suameitria
Mertaniasih, Ni Made
Soedarsono
Hagino, Kimika
Yamazaki, Tomoya
Ozeki, Yuriko
Artama, Wayan Tunas
Kobayashi, Haruka
Inouchi, Erina
Yoshida, Yutaka
Ishikawa, Satoshi
Shaban, Amina Kaboso
Tateishi, Yoshitaka
Nishiyama, Akihito
Ato, Manabu
Matsumoto, Sohkichi
author_sort Dewi, Desak Nyoman Surya Suameitria
collection PubMed
description Accurate point-of-care testing (POCT) is critical for managing tuberculosis (TB). However, current antibody-based diagnosis shows low specificity and sensitivity. To find proper antigen candidates for TB diagnosis by antibodies, we assessed IgGs responsiveness to Mycobacterium tuberculosis proteins in pulmonary TB (PTB) patients. We employed major secreted proteins, such as Rv1860, Ag85C, PstS1, Rv2878c, Ag85B, and Rv1926c that were directly purified from M. tuberculosis. In the first screening, we found that IgG levels were significantly elevated in PTB patients only against Rv1860, PstS1, and Ag85B among tested antigens. However, recombinant PstS1 and Ag85B from Escherichia coli (E. coli) couldn’t distinguish PTB patients and healthy controls (HC). Recombinant Rv1860 was not checked due to its little expression. Then, the 59 confirmed PTB patients from Soetomo General Academic Hospital, Surabaya, Indonesia, and 102 HC were tested to Rv1860 and Ag85B only due to the low yield of the PstS1 from M. tuberculosis. The ROC analysis using native Ag85B and Rv1860 showed an acceptable area under curve for diagnosis, which is 0.812 (95% CI 0.734–0.890, p < 0.0001) and 0.821 (95% CI 0.752–0.890, p < 0.0001). This study indicates that taking consideration of native protein structure is key in developing TB’s POCT by antibody-based diagnosis.
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spelling pubmed-104035042023-08-06 Antibodies against native proteins of Mycobacterium tuberculosis can detect pulmonary tuberculosis patients Dewi, Desak Nyoman Surya Suameitria Mertaniasih, Ni Made Soedarsono Hagino, Kimika Yamazaki, Tomoya Ozeki, Yuriko Artama, Wayan Tunas Kobayashi, Haruka Inouchi, Erina Yoshida, Yutaka Ishikawa, Satoshi Shaban, Amina Kaboso Tateishi, Yoshitaka Nishiyama, Akihito Ato, Manabu Matsumoto, Sohkichi Sci Rep Article Accurate point-of-care testing (POCT) is critical for managing tuberculosis (TB). However, current antibody-based diagnosis shows low specificity and sensitivity. To find proper antigen candidates for TB diagnosis by antibodies, we assessed IgGs responsiveness to Mycobacterium tuberculosis proteins in pulmonary TB (PTB) patients. We employed major secreted proteins, such as Rv1860, Ag85C, PstS1, Rv2878c, Ag85B, and Rv1926c that were directly purified from M. tuberculosis. In the first screening, we found that IgG levels were significantly elevated in PTB patients only against Rv1860, PstS1, and Ag85B among tested antigens. However, recombinant PstS1 and Ag85B from Escherichia coli (E. coli) couldn’t distinguish PTB patients and healthy controls (HC). Recombinant Rv1860 was not checked due to its little expression. Then, the 59 confirmed PTB patients from Soetomo General Academic Hospital, Surabaya, Indonesia, and 102 HC were tested to Rv1860 and Ag85B only due to the low yield of the PstS1 from M. tuberculosis. The ROC analysis using native Ag85B and Rv1860 showed an acceptable area under curve for diagnosis, which is 0.812 (95% CI 0.734–0.890, p < 0.0001) and 0.821 (95% CI 0.752–0.890, p < 0.0001). This study indicates that taking consideration of native protein structure is key in developing TB’s POCT by antibody-based diagnosis. Nature Publishing Group UK 2023-08-04 /pmc/articles/PMC10403504/ /pubmed/37542102 http://dx.doi.org/10.1038/s41598-023-39436-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Dewi, Desak Nyoman Surya Suameitria
Mertaniasih, Ni Made
Soedarsono
Hagino, Kimika
Yamazaki, Tomoya
Ozeki, Yuriko
Artama, Wayan Tunas
Kobayashi, Haruka
Inouchi, Erina
Yoshida, Yutaka
Ishikawa, Satoshi
Shaban, Amina Kaboso
Tateishi, Yoshitaka
Nishiyama, Akihito
Ato, Manabu
Matsumoto, Sohkichi
Antibodies against native proteins of Mycobacterium tuberculosis can detect pulmonary tuberculosis patients
title Antibodies against native proteins of Mycobacterium tuberculosis can detect pulmonary tuberculosis patients
title_full Antibodies against native proteins of Mycobacterium tuberculosis can detect pulmonary tuberculosis patients
title_fullStr Antibodies against native proteins of Mycobacterium tuberculosis can detect pulmonary tuberculosis patients
title_full_unstemmed Antibodies against native proteins of Mycobacterium tuberculosis can detect pulmonary tuberculosis patients
title_short Antibodies against native proteins of Mycobacterium tuberculosis can detect pulmonary tuberculosis patients
title_sort antibodies against native proteins of mycobacterium tuberculosis can detect pulmonary tuberculosis patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10403504/
https://www.ncbi.nlm.nih.gov/pubmed/37542102
http://dx.doi.org/10.1038/s41598-023-39436-4
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