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A single-cell transcriptional landscape of immune cells shows disease-specific changes of T cell and macrophage populations in human achalasia
Achalasia is a rare motility disorder of the esophagus caused by the gradual degeneration of myenteric neurons. Immune-mediated ganglionitis has been proposed to underlie the loss of myenteric neurons. Here, we measure the immune cell transcriptional profile of paired lower esophageal sphincter (LES...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10403544/ https://www.ncbi.nlm.nih.gov/pubmed/37542039 http://dx.doi.org/10.1038/s41467-023-39750-5 |
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author | Liu, Zu-Qiang Dai, Hao Yao, Lu Chen, Wei-Feng Wang, Yun Ma, Li-Yun Li, Xiao-Qing Lin, Sheng-Li He, Meng-Jiang Gao, Ping-Ting Liu, Xin-Yang Xu, Jia-Xin Xu, Xiao-Yue Wang, Ke-Hao Wang, Li Chen, Luonan Zhou, Ping-Hong Li, Quan-Lin |
author_facet | Liu, Zu-Qiang Dai, Hao Yao, Lu Chen, Wei-Feng Wang, Yun Ma, Li-Yun Li, Xiao-Qing Lin, Sheng-Li He, Meng-Jiang Gao, Ping-Ting Liu, Xin-Yang Xu, Jia-Xin Xu, Xiao-Yue Wang, Ke-Hao Wang, Li Chen, Luonan Zhou, Ping-Hong Li, Quan-Lin |
author_sort | Liu, Zu-Qiang |
collection | PubMed |
description | Achalasia is a rare motility disorder of the esophagus caused by the gradual degeneration of myenteric neurons. Immune-mediated ganglionitis has been proposed to underlie the loss of myenteric neurons. Here, we measure the immune cell transcriptional profile of paired lower esophageal sphincter (LES) tissue and blood samples in achalasia and controls using single-cell RNA sequencing (scRNA-seq). In achalasia, we identify a pattern of expanded immune cells and a specific transcriptional phenotype, especially in LES tissue. We show C1QC(+) macrophages and tissue-resident memory T cells (T(RM)), especially ZNF683(+) CD8(+) T(RM) and XCL1(+) CD4(+) T(RM), are significantly expanded and localized surrounding the myenteric plexus in the LES tissue of achalasia. C1QC(+) macrophages are transcriptionally similar to microglia of the central nervous system and have a neurodegenerative dysfunctional phenotype in achalasia. T(RM) also expresses transcripts of dysregulated immune responses in achalasia. Moreover, inflammation increases with disease progression since immune cells are more activated in type I compared with type II achalasia. Thus, we profile the immune cell transcriptional landscape and identify C1QC(+) macrophages and T(RM) as disease-associated immune cell subsets in achalasia. |
format | Online Article Text |
id | pubmed-10403544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-104035442023-08-06 A single-cell transcriptional landscape of immune cells shows disease-specific changes of T cell and macrophage populations in human achalasia Liu, Zu-Qiang Dai, Hao Yao, Lu Chen, Wei-Feng Wang, Yun Ma, Li-Yun Li, Xiao-Qing Lin, Sheng-Li He, Meng-Jiang Gao, Ping-Ting Liu, Xin-Yang Xu, Jia-Xin Xu, Xiao-Yue Wang, Ke-Hao Wang, Li Chen, Luonan Zhou, Ping-Hong Li, Quan-Lin Nat Commun Article Achalasia is a rare motility disorder of the esophagus caused by the gradual degeneration of myenteric neurons. Immune-mediated ganglionitis has been proposed to underlie the loss of myenteric neurons. Here, we measure the immune cell transcriptional profile of paired lower esophageal sphincter (LES) tissue and blood samples in achalasia and controls using single-cell RNA sequencing (scRNA-seq). In achalasia, we identify a pattern of expanded immune cells and a specific transcriptional phenotype, especially in LES tissue. We show C1QC(+) macrophages and tissue-resident memory T cells (T(RM)), especially ZNF683(+) CD8(+) T(RM) and XCL1(+) CD4(+) T(RM), are significantly expanded and localized surrounding the myenteric plexus in the LES tissue of achalasia. C1QC(+) macrophages are transcriptionally similar to microglia of the central nervous system and have a neurodegenerative dysfunctional phenotype in achalasia. T(RM) also expresses transcripts of dysregulated immune responses in achalasia. Moreover, inflammation increases with disease progression since immune cells are more activated in type I compared with type II achalasia. Thus, we profile the immune cell transcriptional landscape and identify C1QC(+) macrophages and T(RM) as disease-associated immune cell subsets in achalasia. Nature Publishing Group UK 2023-08-04 /pmc/articles/PMC10403544/ /pubmed/37542039 http://dx.doi.org/10.1038/s41467-023-39750-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Liu, Zu-Qiang Dai, Hao Yao, Lu Chen, Wei-Feng Wang, Yun Ma, Li-Yun Li, Xiao-Qing Lin, Sheng-Li He, Meng-Jiang Gao, Ping-Ting Liu, Xin-Yang Xu, Jia-Xin Xu, Xiao-Yue Wang, Ke-Hao Wang, Li Chen, Luonan Zhou, Ping-Hong Li, Quan-Lin A single-cell transcriptional landscape of immune cells shows disease-specific changes of T cell and macrophage populations in human achalasia |
title | A single-cell transcriptional landscape of immune cells shows disease-specific changes of T cell and macrophage populations in human achalasia |
title_full | A single-cell transcriptional landscape of immune cells shows disease-specific changes of T cell and macrophage populations in human achalasia |
title_fullStr | A single-cell transcriptional landscape of immune cells shows disease-specific changes of T cell and macrophage populations in human achalasia |
title_full_unstemmed | A single-cell transcriptional landscape of immune cells shows disease-specific changes of T cell and macrophage populations in human achalasia |
title_short | A single-cell transcriptional landscape of immune cells shows disease-specific changes of T cell and macrophage populations in human achalasia |
title_sort | single-cell transcriptional landscape of immune cells shows disease-specific changes of t cell and macrophage populations in human achalasia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10403544/ https://www.ncbi.nlm.nih.gov/pubmed/37542039 http://dx.doi.org/10.1038/s41467-023-39750-5 |
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