Cargando…

Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O(1)

BACKGROUND: The effects of Arula-7 powder (ASP) on diarrhea and intestinal barrier function associated with its regulation of intestinal microflora in calves infected with pathogenic Escherichia coli O(1) (E. coli O(1)) were studied. METHOD: Twenty Holstein calves were randomly divided into four tre...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Hao, Jia, Zhifeng, He, Meiling, Chen, Aorigele, Zhang, Xin, Xu, Jin, Wang, Chunjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10403850/
https://www.ncbi.nlm.nih.gov/pubmed/37542271
http://dx.doi.org/10.1186/s40168-023-01616-9
_version_ 1785085162492526592
author Chen, Hao
Jia, Zhifeng
He, Meiling
Chen, Aorigele
Zhang, Xin
Xu, Jin
Wang, Chunjie
author_facet Chen, Hao
Jia, Zhifeng
He, Meiling
Chen, Aorigele
Zhang, Xin
Xu, Jin
Wang, Chunjie
author_sort Chen, Hao
collection PubMed
description BACKGROUND: The effects of Arula-7 powder (ASP) on diarrhea and intestinal barrier function associated with its regulation of intestinal microflora in calves infected with pathogenic Escherichia coli O(1) (E. coli O(1)) were studied. METHOD: Twenty Holstein calves were randomly divided into four treatment groups: normal control (NC), model control (MC), 0.5 mg/kg ciprofloxacin (CIP) and 2.50 g/kg ASP groups. RESULTS: ASP inhibited the relative abundance of Proteobacteria, Selenomonadales, and Enterobacteriales, and increased the relative abundance of Lactobacillus, Faecalibacterium, and Alloprevotella. Moreover, we demonstrated for the first time that the ASP and CIP promoted weight gain, reduced the diarrhea rate (P < 0.05), and enhanced antioxidant capacity (P < 0.05) due to the increase in average daily gain (ADG), total protein (TP), and albumin (ALB). In addition, ASP and CIP increased the expression of Zunola occludens-1 (ZO-1), Occludin, and Claudin-1 in the ileum (P < 0.05), and improved immunity due to increase levels of interleukin-2 (IL-2), interleukin-4 (IL-4), interferon-γ (IFN-γ), immunoglobulin A (IgA), and immunoglobulin G (IgG) in the serum, strengthened CD4(+)T levels in the ileal mucosa and reducing CD8(+)T and CD11c(+)T (P < 0.05). CONCLUSION: Hence, The intestinal microbiota environment formed by early intervention of ASP powder has a protective effect on the intestinal mucosal function of calves infected with pathogenic E. coli. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40168-023-01616-9.
format Online
Article
Text
id pubmed-10403850
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-104038502023-08-06 Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O(1) Chen, Hao Jia, Zhifeng He, Meiling Chen, Aorigele Zhang, Xin Xu, Jin Wang, Chunjie Microbiome Research BACKGROUND: The effects of Arula-7 powder (ASP) on diarrhea and intestinal barrier function associated with its regulation of intestinal microflora in calves infected with pathogenic Escherichia coli O(1) (E. coli O(1)) were studied. METHOD: Twenty Holstein calves were randomly divided into four treatment groups: normal control (NC), model control (MC), 0.5 mg/kg ciprofloxacin (CIP) and 2.50 g/kg ASP groups. RESULTS: ASP inhibited the relative abundance of Proteobacteria, Selenomonadales, and Enterobacteriales, and increased the relative abundance of Lactobacillus, Faecalibacterium, and Alloprevotella. Moreover, we demonstrated for the first time that the ASP and CIP promoted weight gain, reduced the diarrhea rate (P < 0.05), and enhanced antioxidant capacity (P < 0.05) due to the increase in average daily gain (ADG), total protein (TP), and albumin (ALB). In addition, ASP and CIP increased the expression of Zunola occludens-1 (ZO-1), Occludin, and Claudin-1 in the ileum (P < 0.05), and improved immunity due to increase levels of interleukin-2 (IL-2), interleukin-4 (IL-4), interferon-γ (IFN-γ), immunoglobulin A (IgA), and immunoglobulin G (IgG) in the serum, strengthened CD4(+)T levels in the ileal mucosa and reducing CD8(+)T and CD11c(+)T (P < 0.05). CONCLUSION: Hence, The intestinal microbiota environment formed by early intervention of ASP powder has a protective effect on the intestinal mucosal function of calves infected with pathogenic E. coli. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40168-023-01616-9. BioMed Central 2023-08-05 /pmc/articles/PMC10403850/ /pubmed/37542271 http://dx.doi.org/10.1186/s40168-023-01616-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Chen, Hao
Jia, Zhifeng
He, Meiling
Chen, Aorigele
Zhang, Xin
Xu, Jin
Wang, Chunjie
Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O(1)
title Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O(1)
title_full Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O(1)
title_fullStr Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O(1)
title_full_unstemmed Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O(1)
title_short Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O(1)
title_sort arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic escherichia coli o(1)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10403850/
https://www.ncbi.nlm.nih.gov/pubmed/37542271
http://dx.doi.org/10.1186/s40168-023-01616-9
work_keys_str_mv AT chenhao arula7powderimprovesdiarrheaandintestinalepithelialtightjunctionfunctionassociatedwithitsregulationofintestinalfloraincalvesinfectedwithpathogenicescherichiacolio1
AT jiazhifeng arula7powderimprovesdiarrheaandintestinalepithelialtightjunctionfunctionassociatedwithitsregulationofintestinalfloraincalvesinfectedwithpathogenicescherichiacolio1
AT hemeiling arula7powderimprovesdiarrheaandintestinalepithelialtightjunctionfunctionassociatedwithitsregulationofintestinalfloraincalvesinfectedwithpathogenicescherichiacolio1
AT chenaorigele arula7powderimprovesdiarrheaandintestinalepithelialtightjunctionfunctionassociatedwithitsregulationofintestinalfloraincalvesinfectedwithpathogenicescherichiacolio1
AT zhangxin arula7powderimprovesdiarrheaandintestinalepithelialtightjunctionfunctionassociatedwithitsregulationofintestinalfloraincalvesinfectedwithpathogenicescherichiacolio1
AT xujin arula7powderimprovesdiarrheaandintestinalepithelialtightjunctionfunctionassociatedwithitsregulationofintestinalfloraincalvesinfectedwithpathogenicescherichiacolio1
AT wangchunjie arula7powderimprovesdiarrheaandintestinalepithelialtightjunctionfunctionassociatedwithitsregulationofintestinalfloraincalvesinfectedwithpathogenicescherichiacolio1