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Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States
Between 2018 and 2020, over 100 wild turkey fecal samples were collected from the Eastern and Central thirds of the United States, where commercial turkey production is uncommon. We hypothesized that anticoccidial-sensitive Eimeria spp. would be present in wild turkey fecal samples. Samples containi...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10404749/ https://www.ncbi.nlm.nih.gov/pubmed/37331218 http://dx.doi.org/10.1016/j.psj.2023.102819 |
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author | Trujillo-Peralta, Carolina Ashcraft, Aaron Señas-Cuesta, Roberto Coles, Makenly Hernandez-Velasco, Xochitl Selby, Callie Forga, Aaron Tellez-Isaias, Guillermo Vuong, Christine Bielke, Lisa Barta, John Hargis, Billy Graham, Danielle |
author_facet | Trujillo-Peralta, Carolina Ashcraft, Aaron Señas-Cuesta, Roberto Coles, Makenly Hernandez-Velasco, Xochitl Selby, Callie Forga, Aaron Tellez-Isaias, Guillermo Vuong, Christine Bielke, Lisa Barta, John Hargis, Billy Graham, Danielle |
author_sort | Trujillo-Peralta, Carolina |
collection | PubMed |
description | Between 2018 and 2020, over 100 wild turkey fecal samples were collected from the Eastern and Central thirds of the United States, where commercial turkey production is uncommon. We hypothesized that anticoccidial-sensitive Eimeria spp. would be present in wild turkey fecal samples. Samples containing Eimeria spp. oocysts were amplified in vivo. If propagation was successful, the samples were PCR-speciated and subjected to anticoccidial sensitivity testing (AST) for key members of both ionophore and chemical categories of anticoccidial drugs. The purpose of this study was to isolate Eimeria spp. relevant to commercial turkey production that possessed sensitivity to monensin, zoalene, and amprolium. Future research would evaluate the efficacy of wild turkey Eimeria spp. as vaccine candidates for reducing coccidiosis in commercial turkey flocks utilizing single oocyst-derived stocks obtained in the present study. |
format | Online Article Text |
id | pubmed-10404749 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-104047492023-08-08 Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States Trujillo-Peralta, Carolina Ashcraft, Aaron Señas-Cuesta, Roberto Coles, Makenly Hernandez-Velasco, Xochitl Selby, Callie Forga, Aaron Tellez-Isaias, Guillermo Vuong, Christine Bielke, Lisa Barta, John Hargis, Billy Graham, Danielle Poult Sci IMMUNOLOGY, HEALTH AND DISEASE Between 2018 and 2020, over 100 wild turkey fecal samples were collected from the Eastern and Central thirds of the United States, where commercial turkey production is uncommon. We hypothesized that anticoccidial-sensitive Eimeria spp. would be present in wild turkey fecal samples. Samples containing Eimeria spp. oocysts were amplified in vivo. If propagation was successful, the samples were PCR-speciated and subjected to anticoccidial sensitivity testing (AST) for key members of both ionophore and chemical categories of anticoccidial drugs. The purpose of this study was to isolate Eimeria spp. relevant to commercial turkey production that possessed sensitivity to monensin, zoalene, and amprolium. Future research would evaluate the efficacy of wild turkey Eimeria spp. as vaccine candidates for reducing coccidiosis in commercial turkey flocks utilizing single oocyst-derived stocks obtained in the present study. Elsevier 2023-05-29 /pmc/articles/PMC10404749/ /pubmed/37331218 http://dx.doi.org/10.1016/j.psj.2023.102819 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | IMMUNOLOGY, HEALTH AND DISEASE Trujillo-Peralta, Carolina Ashcraft, Aaron Señas-Cuesta, Roberto Coles, Makenly Hernandez-Velasco, Xochitl Selby, Callie Forga, Aaron Tellez-Isaias, Guillermo Vuong, Christine Bielke, Lisa Barta, John Hargis, Billy Graham, Danielle Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States |
title | Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States |
title_full | Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States |
title_fullStr | Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States |
title_full_unstemmed | Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States |
title_short | Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States |
title_sort | research note: isolation, speciation, and anticoccidial sensitivity of eimeria spp. recovered from wild turkey feces in the united states |
topic | IMMUNOLOGY, HEALTH AND DISEASE |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10404749/ https://www.ncbi.nlm.nih.gov/pubmed/37331218 http://dx.doi.org/10.1016/j.psj.2023.102819 |
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