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Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States

Between 2018 and 2020, over 100 wild turkey fecal samples were collected from the Eastern and Central thirds of the United States, where commercial turkey production is uncommon. We hypothesized that anticoccidial-sensitive Eimeria spp. would be present in wild turkey fecal samples. Samples containi...

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Autores principales: Trujillo-Peralta, Carolina, Ashcraft, Aaron, Señas-Cuesta, Roberto, Coles, Makenly, Hernandez-Velasco, Xochitl, Selby, Callie, Forga, Aaron, Tellez-Isaias, Guillermo, Vuong, Christine, Bielke, Lisa, Barta, John, Hargis, Billy, Graham, Danielle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10404749/
https://www.ncbi.nlm.nih.gov/pubmed/37331218
http://dx.doi.org/10.1016/j.psj.2023.102819
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author Trujillo-Peralta, Carolina
Ashcraft, Aaron
Señas-Cuesta, Roberto
Coles, Makenly
Hernandez-Velasco, Xochitl
Selby, Callie
Forga, Aaron
Tellez-Isaias, Guillermo
Vuong, Christine
Bielke, Lisa
Barta, John
Hargis, Billy
Graham, Danielle
author_facet Trujillo-Peralta, Carolina
Ashcraft, Aaron
Señas-Cuesta, Roberto
Coles, Makenly
Hernandez-Velasco, Xochitl
Selby, Callie
Forga, Aaron
Tellez-Isaias, Guillermo
Vuong, Christine
Bielke, Lisa
Barta, John
Hargis, Billy
Graham, Danielle
author_sort Trujillo-Peralta, Carolina
collection PubMed
description Between 2018 and 2020, over 100 wild turkey fecal samples were collected from the Eastern and Central thirds of the United States, where commercial turkey production is uncommon. We hypothesized that anticoccidial-sensitive Eimeria spp. would be present in wild turkey fecal samples. Samples containing Eimeria spp. oocysts were amplified in vivo. If propagation was successful, the samples were PCR-speciated and subjected to anticoccidial sensitivity testing (AST) for key members of both ionophore and chemical categories of anticoccidial drugs. The purpose of this study was to isolate Eimeria spp. relevant to commercial turkey production that possessed sensitivity to monensin, zoalene, and amprolium. Future research would evaluate the efficacy of wild turkey Eimeria spp. as vaccine candidates for reducing coccidiosis in commercial turkey flocks utilizing single oocyst-derived stocks obtained in the present study.
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spelling pubmed-104047492023-08-08 Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States Trujillo-Peralta, Carolina Ashcraft, Aaron Señas-Cuesta, Roberto Coles, Makenly Hernandez-Velasco, Xochitl Selby, Callie Forga, Aaron Tellez-Isaias, Guillermo Vuong, Christine Bielke, Lisa Barta, John Hargis, Billy Graham, Danielle Poult Sci IMMUNOLOGY, HEALTH AND DISEASE Between 2018 and 2020, over 100 wild turkey fecal samples were collected from the Eastern and Central thirds of the United States, where commercial turkey production is uncommon. We hypothesized that anticoccidial-sensitive Eimeria spp. would be present in wild turkey fecal samples. Samples containing Eimeria spp. oocysts were amplified in vivo. If propagation was successful, the samples were PCR-speciated and subjected to anticoccidial sensitivity testing (AST) for key members of both ionophore and chemical categories of anticoccidial drugs. The purpose of this study was to isolate Eimeria spp. relevant to commercial turkey production that possessed sensitivity to monensin, zoalene, and amprolium. Future research would evaluate the efficacy of wild turkey Eimeria spp. as vaccine candidates for reducing coccidiosis in commercial turkey flocks utilizing single oocyst-derived stocks obtained in the present study. Elsevier 2023-05-29 /pmc/articles/PMC10404749/ /pubmed/37331218 http://dx.doi.org/10.1016/j.psj.2023.102819 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle IMMUNOLOGY, HEALTH AND DISEASE
Trujillo-Peralta, Carolina
Ashcraft, Aaron
Señas-Cuesta, Roberto
Coles, Makenly
Hernandez-Velasco, Xochitl
Selby, Callie
Forga, Aaron
Tellez-Isaias, Guillermo
Vuong, Christine
Bielke, Lisa
Barta, John
Hargis, Billy
Graham, Danielle
Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States
title Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States
title_full Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States
title_fullStr Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States
title_full_unstemmed Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States
title_short Research Note: Isolation, speciation, and anticoccidial sensitivity of Eimeria spp. recovered from wild turkey feces in the United States
title_sort research note: isolation, speciation, and anticoccidial sensitivity of eimeria spp. recovered from wild turkey feces in the united states
topic IMMUNOLOGY, HEALTH AND DISEASE
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10404749/
https://www.ncbi.nlm.nih.gov/pubmed/37331218
http://dx.doi.org/10.1016/j.psj.2023.102819
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