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Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis

The retinal pigment epithelium (RPE) and choroid are located behind the human retina and have multiple functions in the human visual system. Knowledge of the RPE and choroid cells and their gene expression profiles are fundamental for understanding retinal disease mechanisms and therapeutic strategi...

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Detalles Bibliográficos
Autores principales: Huang, Lulin, Ye, Lin, Li, Runze, Zhang, Shanshan, Qu, Chao, Li, Shujin, Li, Jie, Yang, Mu, Wu, Biao, Chen, Ran, Huang, Guo, Gong, Bo, Li, Zheng, Yang, Hongjie, Yu, Man, Shi, Yi, Wang, Changguan, Chen, Wei, Yang, Zhenglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chongqing Medical University 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10404887/
https://www.ncbi.nlm.nih.gov/pubmed/37554187
http://dx.doi.org/10.1016/j.gendis.2022.11.007
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author Huang, Lulin
Ye, Lin
Li, Runze
Zhang, Shanshan
Qu, Chao
Li, Shujin
Li, Jie
Yang, Mu
Wu, Biao
Chen, Ran
Huang, Guo
Gong, Bo
Li, Zheng
Yang, Hongjie
Yu, Man
Shi, Yi
Wang, Changguan
Chen, Wei
Yang, Zhenglin
author_facet Huang, Lulin
Ye, Lin
Li, Runze
Zhang, Shanshan
Qu, Chao
Li, Shujin
Li, Jie
Yang, Mu
Wu, Biao
Chen, Ran
Huang, Guo
Gong, Bo
Li, Zheng
Yang, Hongjie
Yu, Man
Shi, Yi
Wang, Changguan
Chen, Wei
Yang, Zhenglin
author_sort Huang, Lulin
collection PubMed
description The retinal pigment epithelium (RPE) and choroid are located behind the human retina and have multiple functions in the human visual system. Knowledge of the RPE and choroid cells and their gene expression profiles are fundamental for understanding retinal disease mechanisms and therapeutic strategies. Here, we sequenced the RNA of about 0.3 million single cells from human RPE and choroids across two regions and seven ages, revealing regional and age differences within the human RPE and choroid. Cell–cell interactions highlight the broad connectivity networks between the RPE and different choroid cell types. Moreover, the transcription factors and their target genes change during aging. The coding of somatic variations increases during aging in the human RPE and choroid at the single-cell level. Moreover, we identified ELN as a candidate for improving RPE degeneration and choroidal structure during aging. The mapping of the molecular architecture of the human RPE and choroid improves our understanding of the human vision support system and offers potential insights into the intervention targets for retinal diseases.
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spelling pubmed-104048872023-08-08 Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis Huang, Lulin Ye, Lin Li, Runze Zhang, Shanshan Qu, Chao Li, Shujin Li, Jie Yang, Mu Wu, Biao Chen, Ran Huang, Guo Gong, Bo Li, Zheng Yang, Hongjie Yu, Man Shi, Yi Wang, Changguan Chen, Wei Yang, Zhenglin Genes Dis Full Length Article The retinal pigment epithelium (RPE) and choroid are located behind the human retina and have multiple functions in the human visual system. Knowledge of the RPE and choroid cells and their gene expression profiles are fundamental for understanding retinal disease mechanisms and therapeutic strategies. Here, we sequenced the RNA of about 0.3 million single cells from human RPE and choroids across two regions and seven ages, revealing regional and age differences within the human RPE and choroid. Cell–cell interactions highlight the broad connectivity networks between the RPE and different choroid cell types. Moreover, the transcription factors and their target genes change during aging. The coding of somatic variations increases during aging in the human RPE and choroid at the single-cell level. Moreover, we identified ELN as a candidate for improving RPE degeneration and choroidal structure during aging. The mapping of the molecular architecture of the human RPE and choroid improves our understanding of the human vision support system and offers potential insights into the intervention targets for retinal diseases. Chongqing Medical University 2022-12-15 /pmc/articles/PMC10404887/ /pubmed/37554187 http://dx.doi.org/10.1016/j.gendis.2022.11.007 Text en © 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Full Length Article
Huang, Lulin
Ye, Lin
Li, Runze
Zhang, Shanshan
Qu, Chao
Li, Shujin
Li, Jie
Yang, Mu
Wu, Biao
Chen, Ran
Huang, Guo
Gong, Bo
Li, Zheng
Yang, Hongjie
Yu, Man
Shi, Yi
Wang, Changguan
Chen, Wei
Yang, Zhenglin
Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis
title Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis
title_full Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis
title_fullStr Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis
title_full_unstemmed Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis
title_short Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis
title_sort dynamic human retinal pigment epithelium (rpe) and choroid architecture based on single-cell transcriptomic landscape analysis
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10404887/
https://www.ncbi.nlm.nih.gov/pubmed/37554187
http://dx.doi.org/10.1016/j.gendis.2022.11.007
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