Upgraded D22 SEC–SANS setup dedicated to the biology community

Described here is the current status of the upgraded in situ size-exclusion chromatography (SEC) system implemented with the D22 small-angle neutron scattering (SANS) instrument at the Institut Laue–Langevin. Since its initial proof of principle in 2016, this SEC–SANS arrangement has been continuous...

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Detalles Bibliográficos
Autores principales: Martel, Anne, Cocho, Cristina, Caporaletti, Francesca, Jacques, Mark, El Aazzouzi, Abdelali, Lapeyre, Franck, Porcar, Lionel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2023
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10405598/
https://www.ncbi.nlm.nih.gov/pubmed/37555207
http://dx.doi.org/10.1107/S1600576723004119
Descripción
Sumario:Described here is the current status of the upgraded in situ size-exclusion chromatography (SEC) system implemented with the D22 small-angle neutron scattering (SANS) instrument at the Institut Laue–Langevin. Since its initial proof of principle in 2016, this SEC–SANS arrangement has been continuously requested by the user community, leading to the design of an upgraded version. A detailed description of the setup and its control is provided, and a few examples of protein structural investigations are presented, which will highlight the various possibilities and limitations of the setup to optimize experimental success.

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