Upgraded D22 SEC–SANS setup dedicated to the biology community

Described here is the current status of the upgraded in situ size-exclusion chromatography (SEC) system implemented with the D22 small-angle neutron scattering (SANS) instrument at the Institut Laue–Langevin. Since its initial proof of principle in 2016, this SEC–SANS arrangement has been continuous...

Descripción completa

Detalles Bibliográficos
Autores principales: Martel, Anne, Cocho, Cristina, Caporaletti, Francesca, Jacques, Mark, El Aazzouzi, Abdelali, Lapeyre, Franck, Porcar, Lionel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2023
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10405598/
https://www.ncbi.nlm.nih.gov/pubmed/37555207
http://dx.doi.org/10.1107/S1600576723004119
_version_ 1785085569108279296
author Martel, Anne
Cocho, Cristina
Caporaletti, Francesca
Jacques, Mark
El Aazzouzi, Abdelali
Lapeyre, Franck
Porcar, Lionel
author_facet Martel, Anne
Cocho, Cristina
Caporaletti, Francesca
Jacques, Mark
El Aazzouzi, Abdelali
Lapeyre, Franck
Porcar, Lionel
author_sort Martel, Anne
collection PubMed
description Described here is the current status of the upgraded in situ size-exclusion chromatography (SEC) system implemented with the D22 small-angle neutron scattering (SANS) instrument at the Institut Laue–Langevin. Since its initial proof of principle in 2016, this SEC–SANS arrangement has been continuously requested by the user community, leading to the design of an upgraded version. A detailed description of the setup and its control is provided, and a few examples of protein structural investigations are presented, which will highlight the various possibilities and limitations of the setup to optimize experimental success.
format Online
Article
Text
id pubmed-10405598
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher International Union of Crystallography
record_format MEDLINE/PubMed
spelling pubmed-104055982023-08-08 Upgraded D22 SEC–SANS setup dedicated to the biology community Martel, Anne Cocho, Cristina Caporaletti, Francesca Jacques, Mark El Aazzouzi, Abdelali Lapeyre, Franck Porcar, Lionel J Appl Crystallogr Research Papers Described here is the current status of the upgraded in situ size-exclusion chromatography (SEC) system implemented with the D22 small-angle neutron scattering (SANS) instrument at the Institut Laue–Langevin. Since its initial proof of principle in 2016, this SEC–SANS arrangement has been continuously requested by the user community, leading to the design of an upgraded version. A detailed description of the setup and its control is provided, and a few examples of protein structural investigations are presented, which will highlight the various possibilities and limitations of the setup to optimize experimental success. International Union of Crystallography 2023-06-12 /pmc/articles/PMC10405598/ /pubmed/37555207 http://dx.doi.org/10.1107/S1600576723004119 Text en © Anne Martel et al. 2023 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Martel, Anne
Cocho, Cristina
Caporaletti, Francesca
Jacques, Mark
El Aazzouzi, Abdelali
Lapeyre, Franck
Porcar, Lionel
Upgraded D22 SEC–SANS setup dedicated to the biology community
title Upgraded D22 SEC–SANS setup dedicated to the biology community
title_full Upgraded D22 SEC–SANS setup dedicated to the biology community
title_fullStr Upgraded D22 SEC–SANS setup dedicated to the biology community
title_full_unstemmed Upgraded D22 SEC–SANS setup dedicated to the biology community
title_short Upgraded D22 SEC–SANS setup dedicated to the biology community
title_sort upgraded d22 sec–sans setup dedicated to the biology community
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10405598/
https://www.ncbi.nlm.nih.gov/pubmed/37555207
http://dx.doi.org/10.1107/S1600576723004119
work_keys_str_mv AT martelanne upgradedd22secsanssetupdedicatedtothebiologycommunity
AT cochocristina upgradedd22secsanssetupdedicatedtothebiologycommunity
AT caporalettifrancesca upgradedd22secsanssetupdedicatedtothebiologycommunity
AT jacquesmark upgradedd22secsanssetupdedicatedtothebiologycommunity
AT elaazzouziabdelali upgradedd22secsanssetupdedicatedtothebiologycommunity
AT lapeyrefranck upgradedd22secsanssetupdedicatedtothebiologycommunity
AT porcarlionel upgradedd22secsanssetupdedicatedtothebiologycommunity

Ejemplares similares