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Diploid mycelia of Ustilago esculenta fails to maintain sustainable proliferation in host plant

Smut fungi display a uniform life cycle including two phases: a saprophytic phase in vitro and a parasitic phase in host plants. Several apathogenic smut fungi are found, lacking suitable hosts in their habitat. Interestingly, MT-type Ustilago esculenta was found to maintain a parasitic life, lackin...

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Autores principales: Li, Shiyu, Yang, Mengfei, Yao, Tongfu, Xia, Wenqiang, Ye, Zihong, Zhang, Shangfa, Li, Yipeng, Zhang, Zhongjin, Song, Ruiqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10405623/
https://www.ncbi.nlm.nih.gov/pubmed/37555064
http://dx.doi.org/10.3389/fmicb.2023.1199907
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author Li, Shiyu
Yang, Mengfei
Yao, Tongfu
Xia, Wenqiang
Ye, Zihong
Zhang, Shangfa
Li, Yipeng
Zhang, Zhongjin
Song, Ruiqi
author_facet Li, Shiyu
Yang, Mengfei
Yao, Tongfu
Xia, Wenqiang
Ye, Zihong
Zhang, Shangfa
Li, Yipeng
Zhang, Zhongjin
Song, Ruiqi
author_sort Li, Shiyu
collection PubMed
description Smut fungi display a uniform life cycle including two phases: a saprophytic phase in vitro and a parasitic phase in host plants. Several apathogenic smut fungi are found, lacking suitable hosts in their habitat. Interestingly, MT-type Ustilago esculenta was found to maintain a parasitic life, lacking the saprophytic phase. Its long period of asexual proliferation in plant tissue results in severe defects in certain functions. In this study, the growth dynamics of U. esculenta in plant tissues were carefully observed. The mycelia of T- and MT-type U. esculenta exhibit rapid growth after karyogamy and aggregate between cells. While T-type U. esculenta successfully forms teliospores after aggregation, the aggregated mycelia of MT-type U. esculenta gradually disappeared after a short period of massive proliferation. It may be resulted by the lack of nutrition such as glucose and sucrose. After overwintering, infected Zizania latifolia plants no longer contained diploid mycelia resulting from karyogamy. This indicated that diploid mycelia failed to survive in plant tissues. It seems that diploid mycelium only serves to generate teliospores. Notably, MT-type U. esculenta keeps the normal function of karyogamy, though it is not necessary for its asexual life in plant tissue. Further investigations are required to uncover the underlying mechanism, which would improve our understanding of the life cycle of smut fungi and help the breeding of Z. latifolia.
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spelling pubmed-104056232023-08-08 Diploid mycelia of Ustilago esculenta fails to maintain sustainable proliferation in host plant Li, Shiyu Yang, Mengfei Yao, Tongfu Xia, Wenqiang Ye, Zihong Zhang, Shangfa Li, Yipeng Zhang, Zhongjin Song, Ruiqi Front Microbiol Microbiology Smut fungi display a uniform life cycle including two phases: a saprophytic phase in vitro and a parasitic phase in host plants. Several apathogenic smut fungi are found, lacking suitable hosts in their habitat. Interestingly, MT-type Ustilago esculenta was found to maintain a parasitic life, lacking the saprophytic phase. Its long period of asexual proliferation in plant tissue results in severe defects in certain functions. In this study, the growth dynamics of U. esculenta in plant tissues were carefully observed. The mycelia of T- and MT-type U. esculenta exhibit rapid growth after karyogamy and aggregate between cells. While T-type U. esculenta successfully forms teliospores after aggregation, the aggregated mycelia of MT-type U. esculenta gradually disappeared after a short period of massive proliferation. It may be resulted by the lack of nutrition such as glucose and sucrose. After overwintering, infected Zizania latifolia plants no longer contained diploid mycelia resulting from karyogamy. This indicated that diploid mycelia failed to survive in plant tissues. It seems that diploid mycelium only serves to generate teliospores. Notably, MT-type U. esculenta keeps the normal function of karyogamy, though it is not necessary for its asexual life in plant tissue. Further investigations are required to uncover the underlying mechanism, which would improve our understanding of the life cycle of smut fungi and help the breeding of Z. latifolia. Frontiers Media S.A. 2023-07-24 /pmc/articles/PMC10405623/ /pubmed/37555064 http://dx.doi.org/10.3389/fmicb.2023.1199907 Text en Copyright © 2023 Li, Yang, Yao, Xia, Ye, Zhang, Li, Zhang and Song. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Shiyu
Yang, Mengfei
Yao, Tongfu
Xia, Wenqiang
Ye, Zihong
Zhang, Shangfa
Li, Yipeng
Zhang, Zhongjin
Song, Ruiqi
Diploid mycelia of Ustilago esculenta fails to maintain sustainable proliferation in host plant
title Diploid mycelia of Ustilago esculenta fails to maintain sustainable proliferation in host plant
title_full Diploid mycelia of Ustilago esculenta fails to maintain sustainable proliferation in host plant
title_fullStr Diploid mycelia of Ustilago esculenta fails to maintain sustainable proliferation in host plant
title_full_unstemmed Diploid mycelia of Ustilago esculenta fails to maintain sustainable proliferation in host plant
title_short Diploid mycelia of Ustilago esculenta fails to maintain sustainable proliferation in host plant
title_sort diploid mycelia of ustilago esculenta fails to maintain sustainable proliferation in host plant
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10405623/
https://www.ncbi.nlm.nih.gov/pubmed/37555064
http://dx.doi.org/10.3389/fmicb.2023.1199907
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