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FTO promotes proliferation and migration of bladder cancer via enhancing stability of STAT3 mRNA in an m6A-dependent manner

N6-Methyladenosine (m6A) plays a key role in the occurrence and development of various cancers. Fat mass and obesity‐associated protein (FTO) was is involved in multiple cancers owing to its demethylase activity, and the molecular mechanism underlying FTO-promoted bladder cancer proliferation and mi...

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Autores principales: Sun, Zhuang, Sun, Xiaolu, Qin, Guoliang, Li, Yi, Zhou, Guanwen, Jiang, Xianzhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10405749/
https://www.ncbi.nlm.nih.gov/pubmed/37538000
http://dx.doi.org/10.1080/15592294.2023.2242688
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author Sun, Zhuang
Sun, Xiaolu
Qin, Guoliang
Li, Yi
Zhou, Guanwen
Jiang, Xianzhou
author_facet Sun, Zhuang
Sun, Xiaolu
Qin, Guoliang
Li, Yi
Zhou, Guanwen
Jiang, Xianzhou
author_sort Sun, Zhuang
collection PubMed
description N6-Methyladenosine (m6A) plays a key role in the occurrence and development of various cancers. Fat mass and obesity‐associated protein (FTO) was is involved in multiple cancers owing to its demethylase activity, and the molecular mechanism underlying FTO-promoted bladder cancer proliferation and migration via the regulation of RNA stability requires further investigation. In the present study, FTO was upregulated in bladder cancer and related to poor prognosis. Gain- and loss-of-function experiments showed that the upregulation of FTO promoted bladder cancer proliferation and migration. Mechanistic studies showed that FTO enhanced the stability of signal transducer and activator of transcription 3 (STAT3) mRNA in an m6A-dependent manner, thereby increasing STAT3 expression, which subsequently promoted P-STAT3 expression and activated STAT3 signalling pathway. Overall, this study revealed that the critical role of FTO in the progression of bladder cancer and could provide a novel avenue to regulate oncogene STAT3.
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spelling pubmed-104057492023-08-08 FTO promotes proliferation and migration of bladder cancer via enhancing stability of STAT3 mRNA in an m6A-dependent manner Sun, Zhuang Sun, Xiaolu Qin, Guoliang Li, Yi Zhou, Guanwen Jiang, Xianzhou Epigenetics Research Article N6-Methyladenosine (m6A) plays a key role in the occurrence and development of various cancers. Fat mass and obesity‐associated protein (FTO) was is involved in multiple cancers owing to its demethylase activity, and the molecular mechanism underlying FTO-promoted bladder cancer proliferation and migration via the regulation of RNA stability requires further investigation. In the present study, FTO was upregulated in bladder cancer and related to poor prognosis. Gain- and loss-of-function experiments showed that the upregulation of FTO promoted bladder cancer proliferation and migration. Mechanistic studies showed that FTO enhanced the stability of signal transducer and activator of transcription 3 (STAT3) mRNA in an m6A-dependent manner, thereby increasing STAT3 expression, which subsequently promoted P-STAT3 expression and activated STAT3 signalling pathway. Overall, this study revealed that the critical role of FTO in the progression of bladder cancer and could provide a novel avenue to regulate oncogene STAT3. Taylor & Francis 2023-08-04 /pmc/articles/PMC10405749/ /pubmed/37538000 http://dx.doi.org/10.1080/15592294.2023.2242688 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.
spellingShingle Research Article
Sun, Zhuang
Sun, Xiaolu
Qin, Guoliang
Li, Yi
Zhou, Guanwen
Jiang, Xianzhou
FTO promotes proliferation and migration of bladder cancer via enhancing stability of STAT3 mRNA in an m6A-dependent manner
title FTO promotes proliferation and migration of bladder cancer via enhancing stability of STAT3 mRNA in an m6A-dependent manner
title_full FTO promotes proliferation and migration of bladder cancer via enhancing stability of STAT3 mRNA in an m6A-dependent manner
title_fullStr FTO promotes proliferation and migration of bladder cancer via enhancing stability of STAT3 mRNA in an m6A-dependent manner
title_full_unstemmed FTO promotes proliferation and migration of bladder cancer via enhancing stability of STAT3 mRNA in an m6A-dependent manner
title_short FTO promotes proliferation and migration of bladder cancer via enhancing stability of STAT3 mRNA in an m6A-dependent manner
title_sort fto promotes proliferation and migration of bladder cancer via enhancing stability of stat3 mrna in an m6a-dependent manner
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10405749/
https://www.ncbi.nlm.nih.gov/pubmed/37538000
http://dx.doi.org/10.1080/15592294.2023.2242688
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