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Nano Uncaria gambir as Chemopreventive Agent Against Breast Cancer

BACKGROUND: Breast cancer is one of the main causes of death in women. Uncaria gambir is an Indonesian herbal plant that can be used as an anti-cancer. However, herbal medicines have low bioavailability, which affects their bioactivity. Nanoencapsulation can increase bioavailability and stability of...

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Autores principales: Wardana, Andika Pramudya, Aminah, Nanik Siti, Kristanti, Alfinda Novi, Fahmi, Mochamad Zakki, Zahrah, Haninda Iffatuz, Widiyastuti, W, Ajiz, Hendrix Abdul, Zubaidah, Ummi, Wiratama, Priangga Adi, Takaya, Yoshiaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406122/
https://www.ncbi.nlm.nih.gov/pubmed/37555190
http://dx.doi.org/10.2147/IJN.S403385
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author Wardana, Andika Pramudya
Aminah, Nanik Siti
Kristanti, Alfinda Novi
Fahmi, Mochamad Zakki
Zahrah, Haninda Iffatuz
Widiyastuti, W
Ajiz, Hendrix Abdul
Zubaidah, Ummi
Wiratama, Priangga Adi
Takaya, Yoshiaki
author_facet Wardana, Andika Pramudya
Aminah, Nanik Siti
Kristanti, Alfinda Novi
Fahmi, Mochamad Zakki
Zahrah, Haninda Iffatuz
Widiyastuti, W
Ajiz, Hendrix Abdul
Zubaidah, Ummi
Wiratama, Priangga Adi
Takaya, Yoshiaki
author_sort Wardana, Andika Pramudya
collection PubMed
description BACKGROUND: Breast cancer is one of the main causes of death in women. Uncaria gambir is an Indonesian herbal plant that can be used as an anti-cancer. However, herbal medicines have low bioavailability, which affects their bioactivity. Nanoencapsulation can increase bioavailability and stability of bioactive compounds in herbal medicines. PURPOSE: This recent finding tried to unravel anti-cancer and chemopreventive of U. gambir nano-encapsulated by Na-alginate. STUDY DESIGN: U. gambir bioactive compounds were isolated and characterized using UV–Vis spectrometer, FTIR, NMR and HR-MS. U. gambir extract was nanoencapsulated using Na-alginate. Anti-cancer effect was assessed by MTT assay towards T47D cell. Meanwhile, a chemopreventive analysis was carried out in breast cancer mice-induced benzo[α]pyrene. The healthy mice were divided into 8 groups comprising control and treatment. RESULTS: Elucidation of U. gambir ethyl acetate extract confirmed high catechin content, 89.34% (w/w). Successful nanoencapsulation of U. gambir (G-NPs) was indicated. The particle size of G-NPs was 78.40 ± 12.25 nm. Loading efficiency (LE) and loading amount (LA) of G-NPs were 97.56 ± 0.04% and 32.52 ± 0.01%, respectively. G-NPs had an EC(50) value of 10.39 ± 3.50 µg/mL, which was more toxic than the EC(50) value of extract towards the T47D cell line. Administration of 200 mg/kg BW G-NPs to mice induced by benzo[α]pyrene exhibited SOD and GSH levels of 13.69 ng/mL and 455.6 ng/mL. In addition, the lowest TNF-α level was 27.96 ng/mL. A dose of 100 mg/kg BW G-NPs could best increase CAT levels by 7.18 ng/mL. There was no damage or histological abnormalities found in histological analysis of the breast tissue in the group given 200 mg/kg BW G-NPs.
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spelling pubmed-104061222023-08-08 Nano Uncaria gambir as Chemopreventive Agent Against Breast Cancer Wardana, Andika Pramudya Aminah, Nanik Siti Kristanti, Alfinda Novi Fahmi, Mochamad Zakki Zahrah, Haninda Iffatuz Widiyastuti, W Ajiz, Hendrix Abdul Zubaidah, Ummi Wiratama, Priangga Adi Takaya, Yoshiaki Int J Nanomedicine Original Research BACKGROUND: Breast cancer is one of the main causes of death in women. Uncaria gambir is an Indonesian herbal plant that can be used as an anti-cancer. However, herbal medicines have low bioavailability, which affects their bioactivity. Nanoencapsulation can increase bioavailability and stability of bioactive compounds in herbal medicines. PURPOSE: This recent finding tried to unravel anti-cancer and chemopreventive of U. gambir nano-encapsulated by Na-alginate. STUDY DESIGN: U. gambir bioactive compounds were isolated and characterized using UV–Vis spectrometer, FTIR, NMR and HR-MS. U. gambir extract was nanoencapsulated using Na-alginate. Anti-cancer effect was assessed by MTT assay towards T47D cell. Meanwhile, a chemopreventive analysis was carried out in breast cancer mice-induced benzo[α]pyrene. The healthy mice were divided into 8 groups comprising control and treatment. RESULTS: Elucidation of U. gambir ethyl acetate extract confirmed high catechin content, 89.34% (w/w). Successful nanoencapsulation of U. gambir (G-NPs) was indicated. The particle size of G-NPs was 78.40 ± 12.25 nm. Loading efficiency (LE) and loading amount (LA) of G-NPs were 97.56 ± 0.04% and 32.52 ± 0.01%, respectively. G-NPs had an EC(50) value of 10.39 ± 3.50 µg/mL, which was more toxic than the EC(50) value of extract towards the T47D cell line. Administration of 200 mg/kg BW G-NPs to mice induced by benzo[α]pyrene exhibited SOD and GSH levels of 13.69 ng/mL and 455.6 ng/mL. In addition, the lowest TNF-α level was 27.96 ng/mL. A dose of 100 mg/kg BW G-NPs could best increase CAT levels by 7.18 ng/mL. There was no damage or histological abnormalities found in histological analysis of the breast tissue in the group given 200 mg/kg BW G-NPs. Dove 2023-08-03 /pmc/articles/PMC10406122/ /pubmed/37555190 http://dx.doi.org/10.2147/IJN.S403385 Text en © 2023 Wardana et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Wardana, Andika Pramudya
Aminah, Nanik Siti
Kristanti, Alfinda Novi
Fahmi, Mochamad Zakki
Zahrah, Haninda Iffatuz
Widiyastuti, W
Ajiz, Hendrix Abdul
Zubaidah, Ummi
Wiratama, Priangga Adi
Takaya, Yoshiaki
Nano Uncaria gambir as Chemopreventive Agent Against Breast Cancer
title Nano Uncaria gambir as Chemopreventive Agent Against Breast Cancer
title_full Nano Uncaria gambir as Chemopreventive Agent Against Breast Cancer
title_fullStr Nano Uncaria gambir as Chemopreventive Agent Against Breast Cancer
title_full_unstemmed Nano Uncaria gambir as Chemopreventive Agent Against Breast Cancer
title_short Nano Uncaria gambir as Chemopreventive Agent Against Breast Cancer
title_sort nano uncaria gambir as chemopreventive agent against breast cancer
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406122/
https://www.ncbi.nlm.nih.gov/pubmed/37555190
http://dx.doi.org/10.2147/IJN.S403385
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