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The role of EII complex in the bacterial responses to the glucose-survey in clinical Klebsiella pneumoniae isolates

Type 3 fimbriae in Klebsiella pneumoniae are important for bacterial colonization on abiotic and biotic surfaces. The major subunit of type 3 fimbriae (MrkA) is increased by overexpression of EtcABC, an EII complex of phosphoenolpyruvate:carbohydrate phosphotransferase systems (PTSs), through cAMP-c...

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Autores principales: Horng, Yu-Tze, Panjaitan, Novaria Sari Dewi, Tsai, Yi-Jhen, Su, Pin-Wei, Yang, Hung-Chi, Soo, Po-Chi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406186/
https://www.ncbi.nlm.nih.gov/pubmed/37549172
http://dx.doi.org/10.1371/journal.pone.0289759
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author Horng, Yu-Tze
Panjaitan, Novaria Sari Dewi
Tsai, Yi-Jhen
Su, Pin-Wei
Yang, Hung-Chi
Soo, Po-Chi
author_facet Horng, Yu-Tze
Panjaitan, Novaria Sari Dewi
Tsai, Yi-Jhen
Su, Pin-Wei
Yang, Hung-Chi
Soo, Po-Chi
author_sort Horng, Yu-Tze
collection PubMed
description Type 3 fimbriae in Klebsiella pneumoniae are important for bacterial colonization on abiotic and biotic surfaces. The major subunit of type 3 fimbriae (MrkA) is increased by overexpression of EtcABC, an EII complex of phosphoenolpyruvate:carbohydrate phosphotransferase systems (PTSs), through cAMP-cAMP receptor protein (cAMP-CRP) in K. pneumoniae STU1. Here, we further characterized the relations between the amount of etcABC mRNA and MrkA in 78 clinical K. pneumoniae isolates incubated in high levels of glucose. By Western blotting, we observed that MrkA of 29 isolates were not decreased much by high levels of glucose (Group A) but MrkA of other 49 isolates were significantly reduced (Group B) in the same condition. The bacterial biofilms on abiotic surfaces and colonization in the Caenorhabditis elegans of representative isolates in the Group A were not affected by high levels of glucose. However, the biofilm and colonization in the worm of clinical isolates in the Group B were much reduced by high levels of glucose. After quantification by real time RT-PCR, 76% of Group A but just 10% of Group B showed high amount of etcA mRNA. In summary, our results suggested that for most of K. pneumoniae clinical isolates, the amount of etcABC mRNA was positively related to their type 3 fimbriae production in a high level of glucose, thereby to their biofilm formation and colonization in the worm.
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spelling pubmed-104061862023-08-08 The role of EII complex in the bacterial responses to the glucose-survey in clinical Klebsiella pneumoniae isolates Horng, Yu-Tze Panjaitan, Novaria Sari Dewi Tsai, Yi-Jhen Su, Pin-Wei Yang, Hung-Chi Soo, Po-Chi PLoS One Research Article Type 3 fimbriae in Klebsiella pneumoniae are important for bacterial colonization on abiotic and biotic surfaces. The major subunit of type 3 fimbriae (MrkA) is increased by overexpression of EtcABC, an EII complex of phosphoenolpyruvate:carbohydrate phosphotransferase systems (PTSs), through cAMP-cAMP receptor protein (cAMP-CRP) in K. pneumoniae STU1. Here, we further characterized the relations between the amount of etcABC mRNA and MrkA in 78 clinical K. pneumoniae isolates incubated in high levels of glucose. By Western blotting, we observed that MrkA of 29 isolates were not decreased much by high levels of glucose (Group A) but MrkA of other 49 isolates were significantly reduced (Group B) in the same condition. The bacterial biofilms on abiotic surfaces and colonization in the Caenorhabditis elegans of representative isolates in the Group A were not affected by high levels of glucose. However, the biofilm and colonization in the worm of clinical isolates in the Group B were much reduced by high levels of glucose. After quantification by real time RT-PCR, 76% of Group A but just 10% of Group B showed high amount of etcA mRNA. In summary, our results suggested that for most of K. pneumoniae clinical isolates, the amount of etcABC mRNA was positively related to their type 3 fimbriae production in a high level of glucose, thereby to their biofilm formation and colonization in the worm. Public Library of Science 2023-08-07 /pmc/articles/PMC10406186/ /pubmed/37549172 http://dx.doi.org/10.1371/journal.pone.0289759 Text en © 2023 Horng et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Horng, Yu-Tze
Panjaitan, Novaria Sari Dewi
Tsai, Yi-Jhen
Su, Pin-Wei
Yang, Hung-Chi
Soo, Po-Chi
The role of EII complex in the bacterial responses to the glucose-survey in clinical Klebsiella pneumoniae isolates
title The role of EII complex in the bacterial responses to the glucose-survey in clinical Klebsiella pneumoniae isolates
title_full The role of EII complex in the bacterial responses to the glucose-survey in clinical Klebsiella pneumoniae isolates
title_fullStr The role of EII complex in the bacterial responses to the glucose-survey in clinical Klebsiella pneumoniae isolates
title_full_unstemmed The role of EII complex in the bacterial responses to the glucose-survey in clinical Klebsiella pneumoniae isolates
title_short The role of EII complex in the bacterial responses to the glucose-survey in clinical Klebsiella pneumoniae isolates
title_sort role of eii complex in the bacterial responses to the glucose-survey in clinical klebsiella pneumoniae isolates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406186/
https://www.ncbi.nlm.nih.gov/pubmed/37549172
http://dx.doi.org/10.1371/journal.pone.0289759
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