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A standardized protocol for manually segmenting stroke lesions on high-resolution T1-weighted MR images
Although automated methods for stroke lesion segmentation exist, many researchers still rely on manual segmentation as the gold standard. Our detailed, standardized protocol for stroke lesion tracing on high-resolution 3D T1-weighted (T1w) magnetic resonance imaging (MRI) has been used to trace over...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406195/ https://www.ncbi.nlm.nih.gov/pubmed/37555152 http://dx.doi.org/10.3389/fnimg.2022.1098604 |
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author | Lo, Bethany P. Donnelly, Miranda R. Barisano, Giuseppe Liew, Sook-Lei |
author_facet | Lo, Bethany P. Donnelly, Miranda R. Barisano, Giuseppe Liew, Sook-Lei |
author_sort | Lo, Bethany P. |
collection | PubMed |
description | Although automated methods for stroke lesion segmentation exist, many researchers still rely on manual segmentation as the gold standard. Our detailed, standardized protocol for stroke lesion tracing on high-resolution 3D T1-weighted (T1w) magnetic resonance imaging (MRI) has been used to trace over 1,300 stroke MRI. In the current study, we describe the protocol, including a step-by-step method utilized for training multiple individuals to trace lesions (“tracers”) in a consistent manner and suggestions for distinguishing between lesioned and non-lesioned areas in stroke brains. Inter-rater and intra-rater reliability were calculated across six tracers trained using our protocol, resulting in an average intraclass correlation of 0.98 and 0.99, respectively, as well as a Dice similarity coefficient of 0.727 and 0.839, respectively. This protocol provides a standardized guideline for researchers performing manual lesion segmentation in stroke T1-weighted MRI, with detailed methods to promote reproducibility in stroke research. |
format | Online Article Text |
id | pubmed-10406195 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104061952023-08-08 A standardized protocol for manually segmenting stroke lesions on high-resolution T1-weighted MR images Lo, Bethany P. Donnelly, Miranda R. Barisano, Giuseppe Liew, Sook-Lei Front Neuroimaging Neuroimaging Although automated methods for stroke lesion segmentation exist, many researchers still rely on manual segmentation as the gold standard. Our detailed, standardized protocol for stroke lesion tracing on high-resolution 3D T1-weighted (T1w) magnetic resonance imaging (MRI) has been used to trace over 1,300 stroke MRI. In the current study, we describe the protocol, including a step-by-step method utilized for training multiple individuals to trace lesions (“tracers”) in a consistent manner and suggestions for distinguishing between lesioned and non-lesioned areas in stroke brains. Inter-rater and intra-rater reliability were calculated across six tracers trained using our protocol, resulting in an average intraclass correlation of 0.98 and 0.99, respectively, as well as a Dice similarity coefficient of 0.727 and 0.839, respectively. This protocol provides a standardized guideline for researchers performing manual lesion segmentation in stroke T1-weighted MRI, with detailed methods to promote reproducibility in stroke research. Frontiers Media S.A. 2023-01-10 /pmc/articles/PMC10406195/ /pubmed/37555152 http://dx.doi.org/10.3389/fnimg.2022.1098604 Text en Copyright © 2023 Lo, Donnelly, Barisano and Liew. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroimaging Lo, Bethany P. Donnelly, Miranda R. Barisano, Giuseppe Liew, Sook-Lei A standardized protocol for manually segmenting stroke lesions on high-resolution T1-weighted MR images |
title | A standardized protocol for manually segmenting stroke lesions on high-resolution T1-weighted MR images |
title_full | A standardized protocol for manually segmenting stroke lesions on high-resolution T1-weighted MR images |
title_fullStr | A standardized protocol for manually segmenting stroke lesions on high-resolution T1-weighted MR images |
title_full_unstemmed | A standardized protocol for manually segmenting stroke lesions on high-resolution T1-weighted MR images |
title_short | A standardized protocol for manually segmenting stroke lesions on high-resolution T1-weighted MR images |
title_sort | standardized protocol for manually segmenting stroke lesions on high-resolution t1-weighted mr images |
topic | Neuroimaging |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406195/ https://www.ncbi.nlm.nih.gov/pubmed/37555152 http://dx.doi.org/10.3389/fnimg.2022.1098604 |
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