Cargando…

The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation

Sleep is a nearly universal feature of animal behaviour, yet many of the molecular, genetic, and neuronal substrates that orchestrate sleep/wake transitions lie undiscovered. Employing a viral insertion sleep screen in larval zebrafish, we identified a novel gene, dreammist (dmist), whose loss resul...

Descripción completa

Detalles Bibliográficos
Autores principales: Barlow, Ida L, Mackay, Eirinn, Wheater, Emily, Goel, Aimee, Lim, Sumi, Zimmerman, Steve, Woods, Ian, Prober, David A, Rihel, Jason
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406431/
https://www.ncbi.nlm.nih.gov/pubmed/37548652
http://dx.doi.org/10.7554/eLife.87521
Descripción
Sumario:Sleep is a nearly universal feature of animal behaviour, yet many of the molecular, genetic, and neuronal substrates that orchestrate sleep/wake transitions lie undiscovered. Employing a viral insertion sleep screen in larval zebrafish, we identified a novel gene, dreammist (dmist), whose loss results in behavioural hyperactivity and reduced sleep at night. The neuronally expressed dmist gene is conserved across vertebrates and encodes a small single-pass transmembrane protein that is structurally similar to the Na(+),K(+)-ATPase regulator, FXYD1/Phospholemman. Disruption of either fxyd1 or atp1a3a, a Na(+),K(+)-ATPase alpha-3 subunit associated with several heritable movement disorders in humans, led to decreased night-time sleep. Since atpa1a3a and dmist mutants have elevated intracellular Na(+) levels and non-additive effects on sleep amount at night, we propose that Dmist-dependent enhancement of Na(+) pump function modulates neuronal excitability to maintain normal sleep behaviour.