Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging

Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employe...

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Detalles Bibliográficos
Autores principales: Quardokus, Ellen M., Saunders, Diane C., McDonough, Elizabeth, Hickey, John W., Werlein, Christopher, Surrette, Christine, Rajbhandari, Presha, Casals, Anna Martinez, Tian, Hua, Lowery, Lisa, Neumann, Elizabeth K., Björklund, Frida, Neelakantan, Taruna V., Croteau, Josh, Wiblin, Anne E., Fisher, Jeremy, Livengood, April J., Dowell, Karen G., Silverstein, Jonathan C., Spraggins, Jeffrey M., Pryhuber, Gloria S., Deutsch, Gail, Ginty, Fiona, Nolan, Garry P., Melov, Simon, Jonigk, Danny, Caldwell, Michael A., Vlachos, Ioannis S., Muller, Werner, Gehlenborg, Nils, Stockwell, Brent R., Lundberg, Emma, Snyder, Michael P., Germain, Ronald N., Camarillo, Jeannie M., Kelleher, Neil L., Börner, Katy, Radtke, Andrea J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2023
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406602/
https://www.ncbi.nlm.nih.gov/pubmed/37468619
http://dx.doi.org/10.1038/s41592-023-01846-7
Descripción
Sumario:Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.

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