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Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging
Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employe...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group US
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406602/ https://www.ncbi.nlm.nih.gov/pubmed/37468619 http://dx.doi.org/10.1038/s41592-023-01846-7 |
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| author | Quardokus, Ellen M. Saunders, Diane C. McDonough, Elizabeth Hickey, John W. Werlein, Christopher Surrette, Christine Rajbhandari, Presha Casals, Anna Martinez Tian, Hua Lowery, Lisa Neumann, Elizabeth K. Björklund, Frida Neelakantan, Taruna V. Croteau, Josh Wiblin, Anne E. Fisher, Jeremy Livengood, April J. Dowell, Karen G. Silverstein, Jonathan C. Spraggins, Jeffrey M. Pryhuber, Gloria S. Deutsch, Gail Ginty, Fiona Nolan, Garry P. Melov, Simon Jonigk, Danny Caldwell, Michael A. Vlachos, Ioannis S. Muller, Werner Gehlenborg, Nils Stockwell, Brent R. Lundberg, Emma Snyder, Michael P. Germain, Ronald N. Camarillo, Jeannie M. Kelleher, Neil L. Börner, Katy Radtke, Andrea J. |
| author_facet | Quardokus, Ellen M. Saunders, Diane C. McDonough, Elizabeth Hickey, John W. Werlein, Christopher Surrette, Christine Rajbhandari, Presha Casals, Anna Martinez Tian, Hua Lowery, Lisa Neumann, Elizabeth K. Björklund, Frida Neelakantan, Taruna V. Croteau, Josh Wiblin, Anne E. Fisher, Jeremy Livengood, April J. Dowell, Karen G. Silverstein, Jonathan C. Spraggins, Jeffrey M. Pryhuber, Gloria S. Deutsch, Gail Ginty, Fiona Nolan, Garry P. Melov, Simon Jonigk, Danny Caldwell, Michael A. Vlachos, Ioannis S. Muller, Werner Gehlenborg, Nils Stockwell, Brent R. Lundberg, Emma Snyder, Michael P. Germain, Ronald N. Camarillo, Jeannie M. Kelleher, Neil L. Börner, Katy Radtke, Andrea J. |
| author_sort | Quardokus, Ellen M. |
| collection | PubMed |
| description | Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas. |
| format | Online Article Text |
| id | pubmed-10406602 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | Nature Publishing Group US |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-104066022023-08-09 Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging Quardokus, Ellen M. Saunders, Diane C. McDonough, Elizabeth Hickey, John W. Werlein, Christopher Surrette, Christine Rajbhandari, Presha Casals, Anna Martinez Tian, Hua Lowery, Lisa Neumann, Elizabeth K. Björklund, Frida Neelakantan, Taruna V. Croteau, Josh Wiblin, Anne E. Fisher, Jeremy Livengood, April J. Dowell, Karen G. Silverstein, Jonathan C. Spraggins, Jeffrey M. Pryhuber, Gloria S. Deutsch, Gail Ginty, Fiona Nolan, Garry P. Melov, Simon Jonigk, Danny Caldwell, Michael A. Vlachos, Ioannis S. Muller, Werner Gehlenborg, Nils Stockwell, Brent R. Lundberg, Emma Snyder, Michael P. Germain, Ronald N. Camarillo, Jeannie M. Kelleher, Neil L. Börner, Katy Radtke, Andrea J. Nat Methods Brief Communication Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas. Nature Publishing Group US 2023-07-19 2023 /pmc/articles/PMC10406602/ /pubmed/37468619 http://dx.doi.org/10.1038/s41592-023-01846-7 Text en © The Author(s), under exclusive licence to Springer Nature America, Inc. 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
| spellingShingle | Brief Communication Quardokus, Ellen M. Saunders, Diane C. McDonough, Elizabeth Hickey, John W. Werlein, Christopher Surrette, Christine Rajbhandari, Presha Casals, Anna Martinez Tian, Hua Lowery, Lisa Neumann, Elizabeth K. Björklund, Frida Neelakantan, Taruna V. Croteau, Josh Wiblin, Anne E. Fisher, Jeremy Livengood, April J. Dowell, Karen G. Silverstein, Jonathan C. Spraggins, Jeffrey M. Pryhuber, Gloria S. Deutsch, Gail Ginty, Fiona Nolan, Garry P. Melov, Simon Jonigk, Danny Caldwell, Michael A. Vlachos, Ioannis S. Muller, Werner Gehlenborg, Nils Stockwell, Brent R. Lundberg, Emma Snyder, Michael P. Germain, Ronald N. Camarillo, Jeannie M. Kelleher, Neil L. Börner, Katy Radtke, Andrea J. Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging |
| title | Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging |
| title_full | Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging |
| title_fullStr | Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging |
| title_full_unstemmed | Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging |
| title_short | Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging |
| title_sort | organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging |
| topic | Brief Communication |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406602/ https://www.ncbi.nlm.nih.gov/pubmed/37468619 http://dx.doi.org/10.1038/s41592-023-01846-7 |
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