Open-3DSIM: an open-source three-dimensional structured illumination microscopy reconstruction platform

Open-3DSIM is an open-source reconstruction platform for three-dimensional structured illumination microscopy. We demonstrate its superior performance for artifact suppression and high-fidelity reconstruction relative to other algorithms on various specimens and over a range of signal-to-noise level...

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Detalles Bibliográficos
Autores principales: Cao, Ruijie, Li, Yaning, Chen, Xin, Ge, Xichuan, Li, Meiqi, Guan, Meiling, Hou, Yiwei, Fu, Yunzhe, Xu, Xinzhu, Leterrier, Christophe, Jiang, Shan, Gao, Baoxiang, Xi, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2023
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406603/
https://www.ncbi.nlm.nih.gov/pubmed/37474809
http://dx.doi.org/10.1038/s41592-023-01958-0
Descripción
Sumario:Open-3DSIM is an open-source reconstruction platform for three-dimensional structured illumination microscopy. We demonstrate its superior performance for artifact suppression and high-fidelity reconstruction relative to other algorithms on various specimens and over a range of signal-to-noise levels. Open-3DSIM also offers the capacity to extract dipole orientation, paving a new avenue for interpreting subcellular structures in six dimensions (xyzθλt). The platform is available as MATLAB code, a Fiji plugin and an Exe application to maximize user-friendliness.

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