E. coli “Stablelabel” S30 lysate for optimized cell-free NMR sample preparation

Cell-free (CF) synthesis with highly productive E. coli lysates is a convenient method to produce labeled proteins for NMR studies. Despite reduced metabolic activity in CF lysates, a certain scrambling of supplied isotope labels is still notable. Most problematic are conversions of (15)N labels of...

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Detalles Bibliográficos
Autores principales: Levin, Roman, Löhr, Frank, Karakoc, Betül, Lichtenecker, Roman, Dötsch, Volker, Bernhard, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406690/
https://www.ncbi.nlm.nih.gov/pubmed/37311907
http://dx.doi.org/10.1007/s10858-023-00417-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406690/
https://www.ncbi.nlm.nih.gov/pubmed/37311907
http://dx.doi.org/10.1007/s10858-023-00417-4

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