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Technology platform for facile handling of 3D hydrogel cell culture scaffolds
Hydrogels are used extensively as cell-culture scaffolds for both 2D and 3D cell cultures due to their biocompatibility and the ease in which their mechanical and biological properties can be tailored to mimic natural tissue. The challenge when working with hydrogel-based scaffolds is in their handl...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406881/ https://www.ncbi.nlm.nih.gov/pubmed/37550357 http://dx.doi.org/10.1038/s41598-023-39081-x |
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author | Pohlit, Hannah Bohlin, Jan Katiyar, Neeraj Hilborn, Jöns Tenje, Maria |
author_facet | Pohlit, Hannah Bohlin, Jan Katiyar, Neeraj Hilborn, Jöns Tenje, Maria |
author_sort | Pohlit, Hannah |
collection | PubMed |
description | Hydrogels are used extensively as cell-culture scaffolds for both 2D and 3D cell cultures due to their biocompatibility and the ease in which their mechanical and biological properties can be tailored to mimic natural tissue. The challenge when working with hydrogel-based scaffolds is in their handling, as hydrogels that mimic e.g. brain tissue, are both fragile and brittle when prepared as thin (sub-mm) membranes. Here, we describe a method for facile handling of thin hydrogel cell culture scaffolds by molding them onto a polycaprolactone (PCL) mesh support attached to a commonly used Transwell set-up in which the original membrane has been removed. In addition to demonstrating the assembly of this set-up, we also show some applications for this type of biological membrane. A polyethylene glycol (PEG)-gelatin hydrogel supports cell adhesion, and the structures can be used for biological barrier models comprising either one or multiple hydrogel layers. Here, we demonstrate the formation of a tight layer of an epithelial cell model comprising MDCK cells cultured over 9 days by following the build-up of the transepithelial electrical resistances. Second, by integrating a pure PEG hydrogel into the PCL mesh, significant swelling is induced, which leads to the formation of a non-adherent biological scaffold with a large curvature that is useful for spheroid formation. In conclusion, we demonstrate the development of a handling platform for hydrogel cell culture scaffolds for easy integration with conventional measurement techniques and miniaturized organs-on-chip systems. |
format | Online Article Text |
id | pubmed-10406881 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-104068812023-08-09 Technology platform for facile handling of 3D hydrogel cell culture scaffolds Pohlit, Hannah Bohlin, Jan Katiyar, Neeraj Hilborn, Jöns Tenje, Maria Sci Rep Article Hydrogels are used extensively as cell-culture scaffolds for both 2D and 3D cell cultures due to their biocompatibility and the ease in which their mechanical and biological properties can be tailored to mimic natural tissue. The challenge when working with hydrogel-based scaffolds is in their handling, as hydrogels that mimic e.g. brain tissue, are both fragile and brittle when prepared as thin (sub-mm) membranes. Here, we describe a method for facile handling of thin hydrogel cell culture scaffolds by molding them onto a polycaprolactone (PCL) mesh support attached to a commonly used Transwell set-up in which the original membrane has been removed. In addition to demonstrating the assembly of this set-up, we also show some applications for this type of biological membrane. A polyethylene glycol (PEG)-gelatin hydrogel supports cell adhesion, and the structures can be used for biological barrier models comprising either one or multiple hydrogel layers. Here, we demonstrate the formation of a tight layer of an epithelial cell model comprising MDCK cells cultured over 9 days by following the build-up of the transepithelial electrical resistances. Second, by integrating a pure PEG hydrogel into the PCL mesh, significant swelling is induced, which leads to the formation of a non-adherent biological scaffold with a large curvature that is useful for spheroid formation. In conclusion, we demonstrate the development of a handling platform for hydrogel cell culture scaffolds for easy integration with conventional measurement techniques and miniaturized organs-on-chip systems. Nature Publishing Group UK 2023-08-07 /pmc/articles/PMC10406881/ /pubmed/37550357 http://dx.doi.org/10.1038/s41598-023-39081-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Pohlit, Hannah Bohlin, Jan Katiyar, Neeraj Hilborn, Jöns Tenje, Maria Technology platform for facile handling of 3D hydrogel cell culture scaffolds |
title | Technology platform for facile handling of 3D hydrogel cell culture scaffolds |
title_full | Technology platform for facile handling of 3D hydrogel cell culture scaffolds |
title_fullStr | Technology platform for facile handling of 3D hydrogel cell culture scaffolds |
title_full_unstemmed | Technology platform for facile handling of 3D hydrogel cell culture scaffolds |
title_short | Technology platform for facile handling of 3D hydrogel cell culture scaffolds |
title_sort | technology platform for facile handling of 3d hydrogel cell culture scaffolds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406881/ https://www.ncbi.nlm.nih.gov/pubmed/37550357 http://dx.doi.org/10.1038/s41598-023-39081-x |
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