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Multiomics comparison among populations of three plant sources of Amomi Fructus

Amomi Fructus (Sharen, AF) is a traditional Chinese medicine (TCM) from three source species (or varieties), including Wurfbainia villosa var. villosa (WVV), W. villosa var. xanthioides (WVX), or W. longiligularis (WL). Among them, WVV has been transplanted from its top-geoherb region, Guangdong, to...

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Autores principales: Chen, Xinlian, Sun, Shichao, Han, Xiaoxu, Li, Cheng, Wang, Fengjiao, Nie, Bao, Hou, Zhuangwei, Yang, Song, Ji, Jiaojiao, Li, Ge, Wang, Yanqian, Han, Xiaoyu, Yue, Jianjun, Li, Cui, Li, Wei, Zhang, Lixia, Yang, Depo, Wang, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407604/
https://www.ncbi.nlm.nih.gov/pubmed/37560015
http://dx.doi.org/10.1093/hr/uhad128
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author Chen, Xinlian
Sun, Shichao
Han, Xiaoxu
Li, Cheng
Wang, Fengjiao
Nie, Bao
Hou, Zhuangwei
Yang, Song
Ji, Jiaojiao
Li, Ge
Wang, Yanqian
Han, Xiaoyu
Yue, Jianjun
Li, Cui
Li, Wei
Zhang, Lixia
Yang, Depo
Wang, Li
author_facet Chen, Xinlian
Sun, Shichao
Han, Xiaoxu
Li, Cheng
Wang, Fengjiao
Nie, Bao
Hou, Zhuangwei
Yang, Song
Ji, Jiaojiao
Li, Ge
Wang, Yanqian
Han, Xiaoyu
Yue, Jianjun
Li, Cui
Li, Wei
Zhang, Lixia
Yang, Depo
Wang, Li
author_sort Chen, Xinlian
collection PubMed
description Amomi Fructus (Sharen, AF) is a traditional Chinese medicine (TCM) from three source species (or varieties), including Wurfbainia villosa var. villosa (WVV), W. villosa var. xanthioides (WVX), or W. longiligularis (WL). Among them, WVV has been transplanted from its top-geoherb region, Guangdong, to its current main production area, Yunnan, for >50 years in China. However, the genetic and transcriptomic differentiation among multiple AF source species (or varieties) and between the origin and transplanted populations of WVV is unknown. In our study, the observed overall higher expression of terpenoid biosynthesis genes in WVV than in WVX provided possible evidence for the better pharmacological effect of WVV. We also screened six candidate borneol dehydrogenases (BDHs) that potentially catalyzed borneol into camphor in WVV and functionally verified them. Highly expressed genes at the P2 stage of WVV, Wv05G1424 and Wv05G1438, were capable of catalyzing the formation of camphor from (+)-borneol, (−)-borneol and DL-isoborneol. Moreover, the BDH genes may experience independent evolution after acquiring the ancestral copies, and the following tandem duplications might account for the abundant camphor content in WVV. Furthermore, four populations of WVV, WVX, and WL are genetically differentiated, and the gene flow from WVX to WVV in Yunnan contributed to the greater genetic diversity in the introduced population (WVV-JH) than in its top-geoherb region (WVV-YC), which showed the lowest genetic diversity and might undergo genetic degradation. In addition, terpene synthesis (TPS) and BDH genes were selected among populations of multiple AF source species (or varieties) and between the top- and non-top-geoherb regions, which might explain the difference in metabolites between these populations. Our findings provide important guidance for the conservation, genetic improvement, and industrial development of the three source species (or varieties) and for identifying top-geoherbalism with molecular markers, and proper clinical application of AF.
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spelling pubmed-104076042023-08-09 Multiomics comparison among populations of three plant sources of Amomi Fructus Chen, Xinlian Sun, Shichao Han, Xiaoxu Li, Cheng Wang, Fengjiao Nie, Bao Hou, Zhuangwei Yang, Song Ji, Jiaojiao Li, Ge Wang, Yanqian Han, Xiaoyu Yue, Jianjun Li, Cui Li, Wei Zhang, Lixia Yang, Depo Wang, Li Hortic Res Article Amomi Fructus (Sharen, AF) is a traditional Chinese medicine (TCM) from three source species (or varieties), including Wurfbainia villosa var. villosa (WVV), W. villosa var. xanthioides (WVX), or W. longiligularis (WL). Among them, WVV has been transplanted from its top-geoherb region, Guangdong, to its current main production area, Yunnan, for >50 years in China. However, the genetic and transcriptomic differentiation among multiple AF source species (or varieties) and between the origin and transplanted populations of WVV is unknown. In our study, the observed overall higher expression of terpenoid biosynthesis genes in WVV than in WVX provided possible evidence for the better pharmacological effect of WVV. We also screened six candidate borneol dehydrogenases (BDHs) that potentially catalyzed borneol into camphor in WVV and functionally verified them. Highly expressed genes at the P2 stage of WVV, Wv05G1424 and Wv05G1438, were capable of catalyzing the formation of camphor from (+)-borneol, (−)-borneol and DL-isoborneol. Moreover, the BDH genes may experience independent evolution after acquiring the ancestral copies, and the following tandem duplications might account for the abundant camphor content in WVV. Furthermore, four populations of WVV, WVX, and WL are genetically differentiated, and the gene flow from WVX to WVV in Yunnan contributed to the greater genetic diversity in the introduced population (WVV-JH) than in its top-geoherb region (WVV-YC), which showed the lowest genetic diversity and might undergo genetic degradation. In addition, terpene synthesis (TPS) and BDH genes were selected among populations of multiple AF source species (or varieties) and between the top- and non-top-geoherb regions, which might explain the difference in metabolites between these populations. Our findings provide important guidance for the conservation, genetic improvement, and industrial development of the three source species (or varieties) and for identifying top-geoherbalism with molecular markers, and proper clinical application of AF. Oxford University Press 2023-08-01 /pmc/articles/PMC10407604/ /pubmed/37560015 http://dx.doi.org/10.1093/hr/uhad128 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Chen, Xinlian
Sun, Shichao
Han, Xiaoxu
Li, Cheng
Wang, Fengjiao
Nie, Bao
Hou, Zhuangwei
Yang, Song
Ji, Jiaojiao
Li, Ge
Wang, Yanqian
Han, Xiaoyu
Yue, Jianjun
Li, Cui
Li, Wei
Zhang, Lixia
Yang, Depo
Wang, Li
Multiomics comparison among populations of three plant sources of Amomi Fructus
title Multiomics comparison among populations of three plant sources of Amomi Fructus
title_full Multiomics comparison among populations of three plant sources of Amomi Fructus
title_fullStr Multiomics comparison among populations of three plant sources of Amomi Fructus
title_full_unstemmed Multiomics comparison among populations of three plant sources of Amomi Fructus
title_short Multiomics comparison among populations of three plant sources of Amomi Fructus
title_sort multiomics comparison among populations of three plant sources of amomi fructus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407604/
https://www.ncbi.nlm.nih.gov/pubmed/37560015
http://dx.doi.org/10.1093/hr/uhad128
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