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Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage
Liver as iron storage organ is particularly susceptible to oxidative stress-induced injury from excess iron. Thus, antioxidant therapies are often used to reverse oxidative damage and protect cells and tissues. This study investigated the protective effects of phenolic acids; ferulic acid (FA) and i...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407627/ https://www.ncbi.nlm.nih.gov/pubmed/37560439 http://dx.doi.org/10.1016/j.bbrep.2023.101521 |
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author | Kose, Tugba Moreno-Fernandez, Jorge Vera-Aviles, Mayra Sharp, Paul A. Latunde-Dada, Gladys O. |
author_facet | Kose, Tugba Moreno-Fernandez, Jorge Vera-Aviles, Mayra Sharp, Paul A. Latunde-Dada, Gladys O. |
author_sort | Kose, Tugba |
collection | PubMed |
description | Liver as iron storage organ is particularly susceptible to oxidative stress-induced injury from excess iron. Thus, antioxidant therapies are often used to reverse oxidative damage and protect cells and tissues. This study investigated the protective effects of phenolic acids; ferulic acid (FA) and its metabolite, ferulic acid 4-O-sulfate disodium salt (FAS) against oxidative stress under iron overload conditions in mouse and HepG2 cells. Cells were exposed to FA or FAS and then treated with iron-induced oxidative stress complex of 50 μmol/L FAC and 20 μmol/L of 8-hydroxyquinoline 8HQ (8HQ-FAC). Iron dextran was injected intraperitoneally on alternate days for 10 days to induce the iron overload condition in BALB/c mice. The study revealed that the phenolic acids were protective against ROS production, lipid peroxidation and antioxidant depletion in HepG2 cells and liver tissues of BALB/c mice during iron-induced oxidative stress. The protective function of phenolic acids was achieved by the transcriptional activation of nuclear factor erythroid-2-related factor 2 (Nrf2) to regulate antioxidant genes. In conclusion, the study provides evidence that FA has the potential as a therapeutic agent against iron-related diseases such as T2D. |
format | Online Article Text |
id | pubmed-10407627 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-104076272023-08-09 Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage Kose, Tugba Moreno-Fernandez, Jorge Vera-Aviles, Mayra Sharp, Paul A. Latunde-Dada, Gladys O. Biochem Biophys Rep Research Article Liver as iron storage organ is particularly susceptible to oxidative stress-induced injury from excess iron. Thus, antioxidant therapies are often used to reverse oxidative damage and protect cells and tissues. This study investigated the protective effects of phenolic acids; ferulic acid (FA) and its metabolite, ferulic acid 4-O-sulfate disodium salt (FAS) against oxidative stress under iron overload conditions in mouse and HepG2 cells. Cells were exposed to FA or FAS and then treated with iron-induced oxidative stress complex of 50 μmol/L FAC and 20 μmol/L of 8-hydroxyquinoline 8HQ (8HQ-FAC). Iron dextran was injected intraperitoneally on alternate days for 10 days to induce the iron overload condition in BALB/c mice. The study revealed that the phenolic acids were protective against ROS production, lipid peroxidation and antioxidant depletion in HepG2 cells and liver tissues of BALB/c mice during iron-induced oxidative stress. The protective function of phenolic acids was achieved by the transcriptional activation of nuclear factor erythroid-2-related factor 2 (Nrf2) to regulate antioxidant genes. In conclusion, the study provides evidence that FA has the potential as a therapeutic agent against iron-related diseases such as T2D. Elsevier 2023-07-31 /pmc/articles/PMC10407627/ /pubmed/37560439 http://dx.doi.org/10.1016/j.bbrep.2023.101521 Text en © 2023 Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Kose, Tugba Moreno-Fernandez, Jorge Vera-Aviles, Mayra Sharp, Paul A. Latunde-Dada, Gladys O. Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage |
title | Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage |
title_full | Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage |
title_fullStr | Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage |
title_full_unstemmed | Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage |
title_short | Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage |
title_sort | ferulic acid protects hepg2 cells and mouse liver from iron-induced damage |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407627/ https://www.ncbi.nlm.nih.gov/pubmed/37560439 http://dx.doi.org/10.1016/j.bbrep.2023.101521 |
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