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Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage

Liver as iron storage organ is particularly susceptible to oxidative stress-induced injury from excess iron. Thus, antioxidant therapies are often used to reverse oxidative damage and protect cells and tissues. This study investigated the protective effects of phenolic acids; ferulic acid (FA) and i...

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Autores principales: Kose, Tugba, Moreno-Fernandez, Jorge, Vera-Aviles, Mayra, Sharp, Paul A., Latunde-Dada, Gladys O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407627/
https://www.ncbi.nlm.nih.gov/pubmed/37560439
http://dx.doi.org/10.1016/j.bbrep.2023.101521
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author Kose, Tugba
Moreno-Fernandez, Jorge
Vera-Aviles, Mayra
Sharp, Paul A.
Latunde-Dada, Gladys O.
author_facet Kose, Tugba
Moreno-Fernandez, Jorge
Vera-Aviles, Mayra
Sharp, Paul A.
Latunde-Dada, Gladys O.
author_sort Kose, Tugba
collection PubMed
description Liver as iron storage organ is particularly susceptible to oxidative stress-induced injury from excess iron. Thus, antioxidant therapies are often used to reverse oxidative damage and protect cells and tissues. This study investigated the protective effects of phenolic acids; ferulic acid (FA) and its metabolite, ferulic acid 4-O-sulfate disodium salt (FAS) against oxidative stress under iron overload conditions in mouse and HepG2 cells. Cells were exposed to FA or FAS and then treated with iron-induced oxidative stress complex of 50 μmol/L FAC and 20 μmol/L of 8-hydroxyquinoline 8HQ (8HQ-FAC). Iron dextran was injected intraperitoneally on alternate days for 10 days to induce the iron overload condition in BALB/c mice. The study revealed that the phenolic acids were protective against ROS production, lipid peroxidation and antioxidant depletion in HepG2 cells and liver tissues of BALB/c mice during iron-induced oxidative stress. The protective function of phenolic acids was achieved by the transcriptional activation of nuclear factor erythroid-2-related factor 2 (Nrf2) to regulate antioxidant genes. In conclusion, the study provides evidence that FA has the potential as a therapeutic agent against iron-related diseases such as T2D.
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spelling pubmed-104076272023-08-09 Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage Kose, Tugba Moreno-Fernandez, Jorge Vera-Aviles, Mayra Sharp, Paul A. Latunde-Dada, Gladys O. Biochem Biophys Rep Research Article Liver as iron storage organ is particularly susceptible to oxidative stress-induced injury from excess iron. Thus, antioxidant therapies are often used to reverse oxidative damage and protect cells and tissues. This study investigated the protective effects of phenolic acids; ferulic acid (FA) and its metabolite, ferulic acid 4-O-sulfate disodium salt (FAS) against oxidative stress under iron overload conditions in mouse and HepG2 cells. Cells were exposed to FA or FAS and then treated with iron-induced oxidative stress complex of 50 μmol/L FAC and 20 μmol/L of 8-hydroxyquinoline 8HQ (8HQ-FAC). Iron dextran was injected intraperitoneally on alternate days for 10 days to induce the iron overload condition in BALB/c mice. The study revealed that the phenolic acids were protective against ROS production, lipid peroxidation and antioxidant depletion in HepG2 cells and liver tissues of BALB/c mice during iron-induced oxidative stress. The protective function of phenolic acids was achieved by the transcriptional activation of nuclear factor erythroid-2-related factor 2 (Nrf2) to regulate antioxidant genes. In conclusion, the study provides evidence that FA has the potential as a therapeutic agent against iron-related diseases such as T2D. Elsevier 2023-07-31 /pmc/articles/PMC10407627/ /pubmed/37560439 http://dx.doi.org/10.1016/j.bbrep.2023.101521 Text en © 2023 Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Kose, Tugba
Moreno-Fernandez, Jorge
Vera-Aviles, Mayra
Sharp, Paul A.
Latunde-Dada, Gladys O.
Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage
title Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage
title_full Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage
title_fullStr Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage
title_full_unstemmed Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage
title_short Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage
title_sort ferulic acid protects hepg2 cells and mouse liver from iron-induced damage
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407627/
https://www.ncbi.nlm.nih.gov/pubmed/37560439
http://dx.doi.org/10.1016/j.bbrep.2023.101521
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