Precision RNAi using synthetic shRNAmir target sites

Loss-of-function genetic tools are widely applied for validating therapeutic targets, but their utility remains limited by incomplete on- and uncontrolled off-target effects. We describe artificial RNA interference (ARTi) based on synthetic, ultra-potent, off-target-free shRNAs that enable efficient...

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Detalles Bibliográficos
Autores principales: Hoffmann, Thomas, Hörmann, Alexandra, Corcokovic, Maja, Zmajkovic, Jakub, Hinterndorfer, Matthias, Salkanovic, Jasko, Spreitzer, Fiona, Köferle, Anna, Gitschtaler, Katrin, Popa, Alexandra, Oberndorfer, Sarah, Andersch, Florian, Schaefer, Markus, Fellner, Michaela, Budano, Nicole, Ruppert, Jan G, Chetta, Paolo, Wurm, Melanie, Zuber, Johannes, Neumüller, Ralph A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Cancer Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10409502/
https://www.ncbi.nlm.nih.gov/pubmed/37552050
http://dx.doi.org/10.7554/eLife.84792

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