Cargando…
Mechanisms governing the impact of nitrogen stress on the formation of secondary metabolites in Artemisia argyi leaves
Nitrogen is a key factor in various physiological and metabolic processes in plants. Providing an adequate supply of nitrogen is essential for improving the total yield and quality of the medicinal plant Artemisia argyi (A. argyi), but the underlying mechanisms of how this nutrient alters the crop r...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10409738/ https://www.ncbi.nlm.nih.gov/pubmed/37553416 http://dx.doi.org/10.1038/s41598-023-40098-5 |
_version_ | 1785086309234114560 |
---|---|
author | Wang, Zixin Zhao, Tingting Ma, Lin Chen, Changjie Miao, Yuhuan Guo, Lanping Liu, Dahui |
author_facet | Wang, Zixin Zhao, Tingting Ma, Lin Chen, Changjie Miao, Yuhuan Guo, Lanping Liu, Dahui |
author_sort | Wang, Zixin |
collection | PubMed |
description | Nitrogen is a key factor in various physiological and metabolic processes in plants. Providing an adequate supply of nitrogen is essential for improving the total yield and quality of the medicinal plant Artemisia argyi (A. argyi), but the underlying mechanisms of how this nutrient alters the crop remains unclear. In this study, we conducted a series of pot experiments to investigate the agronomic traits and active components in the leaves of A. argyi plants under low and high nitrogen stress. Additionally, we used transcriptome analysis and RT-qPCR to explore the molecular pathways associated with nitrogen stress. Our results demonstrate a dramatic increase in the accumulation of phenolic acids and flavonoids in the low nitrogen (LN) stress group compared to the control (CK), with increases of 40.00% and 79.49%, respectively. Interestingly, plants in the high nitrogen (HN) stress group exhibited enhanced plant growth with larger leaves, thicker stems, and a 3% increase in volatile oil content compared to the CK. Moreover, A. argyi in the HN group displayed a 66% increase in volatile oil concentration compared to the LN group. Our combined transcriptome and q-PCR results indicate that LN stress promotes the expression of genes involved in flavonoid synthesis, while HN stress promotes the expression of genes related to terpene skeleton production and photosynthesis. Taken together, these findings suggest that different gene expression levels under LN and HN stress contribute to the photosynthesis capacity and the accumulation of active ingredients in A. argyi leaves. Our results elucidate the physiological and molecular mechanisms of nitrogen stress on A. argyi secondary metabolites and guide fertilization strategies for plant cultivation. |
format | Online Article Text |
id | pubmed-10409738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-104097382023-08-10 Mechanisms governing the impact of nitrogen stress on the formation of secondary metabolites in Artemisia argyi leaves Wang, Zixin Zhao, Tingting Ma, Lin Chen, Changjie Miao, Yuhuan Guo, Lanping Liu, Dahui Sci Rep Article Nitrogen is a key factor in various physiological and metabolic processes in plants. Providing an adequate supply of nitrogen is essential for improving the total yield and quality of the medicinal plant Artemisia argyi (A. argyi), but the underlying mechanisms of how this nutrient alters the crop remains unclear. In this study, we conducted a series of pot experiments to investigate the agronomic traits and active components in the leaves of A. argyi plants under low and high nitrogen stress. Additionally, we used transcriptome analysis and RT-qPCR to explore the molecular pathways associated with nitrogen stress. Our results demonstrate a dramatic increase in the accumulation of phenolic acids and flavonoids in the low nitrogen (LN) stress group compared to the control (CK), with increases of 40.00% and 79.49%, respectively. Interestingly, plants in the high nitrogen (HN) stress group exhibited enhanced plant growth with larger leaves, thicker stems, and a 3% increase in volatile oil content compared to the CK. Moreover, A. argyi in the HN group displayed a 66% increase in volatile oil concentration compared to the LN group. Our combined transcriptome and q-PCR results indicate that LN stress promotes the expression of genes involved in flavonoid synthesis, while HN stress promotes the expression of genes related to terpene skeleton production and photosynthesis. Taken together, these findings suggest that different gene expression levels under LN and HN stress contribute to the photosynthesis capacity and the accumulation of active ingredients in A. argyi leaves. Our results elucidate the physiological and molecular mechanisms of nitrogen stress on A. argyi secondary metabolites and guide fertilization strategies for plant cultivation. Nature Publishing Group UK 2023-08-08 /pmc/articles/PMC10409738/ /pubmed/37553416 http://dx.doi.org/10.1038/s41598-023-40098-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Wang, Zixin Zhao, Tingting Ma, Lin Chen, Changjie Miao, Yuhuan Guo, Lanping Liu, Dahui Mechanisms governing the impact of nitrogen stress on the formation of secondary metabolites in Artemisia argyi leaves |
title | Mechanisms governing the impact of nitrogen stress on the formation of secondary metabolites in Artemisia argyi leaves |
title_full | Mechanisms governing the impact of nitrogen stress on the formation of secondary metabolites in Artemisia argyi leaves |
title_fullStr | Mechanisms governing the impact of nitrogen stress on the formation of secondary metabolites in Artemisia argyi leaves |
title_full_unstemmed | Mechanisms governing the impact of nitrogen stress on the formation of secondary metabolites in Artemisia argyi leaves |
title_short | Mechanisms governing the impact of nitrogen stress on the formation of secondary metabolites in Artemisia argyi leaves |
title_sort | mechanisms governing the impact of nitrogen stress on the formation of secondary metabolites in artemisia argyi leaves |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10409738/ https://www.ncbi.nlm.nih.gov/pubmed/37553416 http://dx.doi.org/10.1038/s41598-023-40098-5 |
work_keys_str_mv | AT wangzixin mechanismsgoverningtheimpactofnitrogenstressontheformationofsecondarymetabolitesinartemisiaargyileaves AT zhaotingting mechanismsgoverningtheimpactofnitrogenstressontheformationofsecondarymetabolitesinartemisiaargyileaves AT malin mechanismsgoverningtheimpactofnitrogenstressontheformationofsecondarymetabolitesinartemisiaargyileaves AT chenchangjie mechanismsgoverningtheimpactofnitrogenstressontheformationofsecondarymetabolitesinartemisiaargyileaves AT miaoyuhuan mechanismsgoverningtheimpactofnitrogenstressontheformationofsecondarymetabolitesinartemisiaargyileaves AT guolanping mechanismsgoverningtheimpactofnitrogenstressontheformationofsecondarymetabolitesinartemisiaargyileaves AT liudahui mechanismsgoverningtheimpactofnitrogenstressontheformationofsecondarymetabolitesinartemisiaargyileaves |