Cargando…

spinDrop: a droplet microfluidic platform to maximise single-cell sequencing information content

Droplet microfluidic methods have massively increased the throughput of single-cell sequencing campaigns. The benefit of scale-up is, however, accompanied by increased background noise when processing challenging samples and the overall RNA capture efficiency is lower. These drawbacks stem from the...

Descripción completa

Detalles Bibliográficos
Autores principales:	De Jonghe, Joachim, Kaminski, Tomasz S., Morse, David B., Tabaka, Marcin, Ellermann, Anna L., Kohler, Timo N., Amadei, Gianluca, Handford, Charlotte E., Findlay, Gregory M., Zernicka-Goetz, Magdalena, Teichmann, Sarah A., Hollfelder, Florian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10409775/ https://www.ncbi.nlm.nih.gov/pubmed/37553326 http://dx.doi.org/10.1038/s41467-023-40322-w

Ejemplares similares

Generation of liver mesenchyme and ductal cell organoid co-culture using cell self-aggregation and droplet microfluidics
por: Dowbaj, Anna M., et al.
Publicado: (2023)

Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro
por: Amadei, Gianluca, et al.
Publicado: (2021)

Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets
por: Ladeveze, Simon, et al.
Publicado: (2023)

Ultrahigh‐Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay
por: Klaus, Miriam, et al.
Publicado: (2021)

Embryo model completes gastrulation to neurulation and organogenesis
por: Amadei, Gianluca, et al.
Publicado: (2022)

Investigating host-microbiome interactions by droplet based microfluidics
por: Tauzin, Alexandra S., et al.
Publicado: (2020)

Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance
por: Richter, Esther S., et al.
Publicado: (2022)

Biocompatibility of fluids for multiphase drops-in-drops microfluidics
por: Prastowo, Aishah, et al.
Publicado: (2016)

Ultra-High-Throughput Absorbance-Activated Droplet Sorting for Enzyme Screening at Kilohertz Frequencies
por: Medcalf, Elliot J., et al.
Publicado: (2023)

An Automated Microfluidic System for the Generation of Droplet Interface Bilayer Networks
por: Czekalska, Magdalena A., et al.
Publicado: (2017)

Microfluidic Chamber Design for Controlled Droplet Expansion and Coalescence
por: Kielpinski, Mark, et al.
Publicado: (2020)

Maximising the potential of neuroimmunology
por: Caldwell, Lindsey J., et al.
Publicado: (2020)

Microfluidic Droplet-Storage Array
por: Rho, Hoon Suk, et al.
Publicado: (2020)

Robotic automation of droplet microfluidics
por: Tran, Tuan M., et al.
Publicado: (2022)

High-throughput total RNA sequencing in single cells using VASA-seq
por: Salmen, Fredrik, et al.
Publicado: (2022)

Vector redesign and in‐droplet cell‐growth improves enrichment and recovery in live Escherichia coli
por: Eenink, Bernard D. G., et al.
Publicado: (2022)

Circuit-Based Design of Microfluidic Drop Networks
por: Rousset, Nassim, et al.
Publicado: (2022)

Maximise your mind power
por: Rees, Jim
Publicado: (2007)

Strategies for maximising energy and luminosity
por: Lamont, M
Publicado: (2000)

Droplet Microfluidics for Chip-Based Diagnostics
por: Kaler, Karan V. I. S., et al.
Publicado: (2014)

Microfluidic droplet enrichment for targeted sequencing
por: Eastburn, Dennis J., et al.
Publicado: (2015)

Dual-nozzle microfluidic droplet generator
por: Choi, Ji Wook, et al.
Publicado: (2018)

Droplet Microfluidics for the Production of Microparticles and Nanoparticles
por: Wang, Jianmei, et al.
Publicado: (2017)

Microfluidic Droplet Extraction by Hydrophilic Membrane
por: Feng, Shilun, et al.
Publicado: (2017)

Editorial for the Special Issue on Droplet Microfluidics
por: Brouzes, Eric, et al.
Publicado: (2020)

Droplet Microfluidics for Food and Nutrition Applications
por: Schroen, Karin, et al.
Publicado: (2021)

Two-Phase Biocatalysis in Microfluidic Droplets
por: Xiang, Lanting, et al.
Publicado: (2021)

Droplet Microfluidic Device for Chemoenzymatic Sensing
por: Yakimov, Anton S., et al.
Publicado: (2022)

Droplet-Based Microfluidics: Applications in Pharmaceuticals
por: Trinh, Thi Ngoc Diep, et al.
Publicado: (2023)

Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension
por: Bao, Min, et al.
Publicado: (2022)

Author Correction: Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension
por: Bao, Min, et al.
Publicado: (2023)

Dropping in on lipid droplets: insights into cellular stress and cancer
por: Shyu, Peter, et al.
Publicado: (2018)

Agarose microgel culture delineates lumenogenesis in naive and primed human pluripotent stem cells
por: Schindler, Magdalena, et al.
Publicado: (2021)

Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets
por: Gielen, Fabrice, et al.
Publicado: (2017)

DropSOAC: Stabilizing Microfluidic Drops for Time-Lapse Quantification of Single-Cell Bacterial Physiology
por: Pratt, Shawna L., et al.
Publicado: (2019)

The Effect of Oil Viscosity on Droplet Generation Rate and Droplet Size in a T-Junction Microfluidic Droplet Generator
por: Yao, Junyi, et al.
Publicado: (2019)

An intra-droplet particle switch for droplet microfluidics using bulk acoustic waves
por: Fornell, Anna, et al.
Publicado: (2017)

Microfluidic Device for Droplet Pairing by Combining Droplet Railing and Floating Trap Arrays
por: Duchamp, Margaux, et al.
Publicado: (2021)

Evolution and Single‐Droplet Analysis of Fuel‐Driven Compartments by Droplet‐Based Microfluidics
por: Bergmann, Alexander M., et al.
Publicado: (2022)

Fluorescence detection methods for microfluidic droplet platforms
por: Casadevall i Solvas, Xavier, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_99l6e1m7q0np01m4t4h1vi3gq4