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Dehydrozingerone promotes healing of diabetic foot ulcers: a molecular insight

INTRODUCTION: One of the most common problems of diabetes are diabetic foot ulcers (DFUs). According to National Institute for Health, initial management of DFUs can decrease the complication of limb amputations and can improve the patient’s quality of life. DFU treatment can be optimized with the h...

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Autores principales: Begum, Farmiza, Manandhar, Suman, Kumar, Gautam, Keni, Raghuvir, Sankhe, Runali, Gurram, Prasada Chowdari, Beegum, Fathima, Teja, Meka Sai, Nandakumar, Krishnadas, Shenoy, Rekha R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10409929/
https://www.ncbi.nlm.nih.gov/pubmed/36280629
http://dx.doi.org/10.1007/s12079-022-00703-0
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author Begum, Farmiza
Manandhar, Suman
Kumar, Gautam
Keni, Raghuvir
Sankhe, Runali
Gurram, Prasada Chowdari
Beegum, Fathima
Teja, Meka Sai
Nandakumar, Krishnadas
Shenoy, Rekha R.
author_facet Begum, Farmiza
Manandhar, Suman
Kumar, Gautam
Keni, Raghuvir
Sankhe, Runali
Gurram, Prasada Chowdari
Beegum, Fathima
Teja, Meka Sai
Nandakumar, Krishnadas
Shenoy, Rekha R.
author_sort Begum, Farmiza
collection PubMed
description INTRODUCTION: One of the most common problems of diabetes are diabetic foot ulcers (DFUs). According to National Institute for Health, initial management of DFUs can decrease the complication of limb amputations and can improve the patient’s quality of life. DFU treatment can be optimized with the help of multidisciplinary approach. Based on many studies, control of glucose levels in blood, antioxidant activity, reduction in cytokine levels, re-epithelialization, collagen formation, migration of fibroblasts are major phases involved in managing DFU. Dehydrozingerone (DHZ), has been known for its anti-inflammatory, antioxidant and wound healing properties. METHODOLOGY: Three months high-fat diet and low dose of streptozotocin-induced type-II diabetic foot ulcer model was used to evaluate the effectiveness of dehydrozingerone. DHZ was given orally to rats for 15 days post wounding. TNF-α, IL-1β and antioxidant parameters like lipid peroxidation, glutathione reductase were estimated. Immunoblotting was done to investigate the effect of DHZ on the expression of ERK, JNK, HSP-27, P38, SIRT-1, NFκB, SMA, VEGF and MMP-9 in skin tissue. Histopathology was performed for analyzing DHZ effect on migration of fibroblasts, formation of epithelium, granulation tissue formation, angiogenesis and collagen formation. RESULTS: DHZ decreased the levels of malondialdehyde, TNF-α, IL-1β and increased glutathione levels in wound tissue. Western blotting results suggested that DHZ activated ERK1/2/JNK/p38 signaling, increased expression of HSP-27, SIRT-1, VEGF, SMA thus facilitating the migration and proliferation of fibroblasts, angiogenesis and decreased inflammation. Masson Trichrome & histopathology showed an increase in collagen, epithelial and granulation tissue formation. CONCLUSION: DHZ significantly accelerates the healing of diabetic foot ulcers in high fat diet fed plus low dose streptozotocin induced type-II diabetic Wistar rats. GRAPHICAL ABSTRACT: [Image: see text]
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spelling pubmed-104099292023-08-10 Dehydrozingerone promotes healing of diabetic foot ulcers: a molecular insight Begum, Farmiza Manandhar, Suman Kumar, Gautam Keni, Raghuvir Sankhe, Runali Gurram, Prasada Chowdari Beegum, Fathima Teja, Meka Sai Nandakumar, Krishnadas Shenoy, Rekha R. J Cell Commun Signal Research Article INTRODUCTION: One of the most common problems of diabetes are diabetic foot ulcers (DFUs). According to National Institute for Health, initial management of DFUs can decrease the complication of limb amputations and can improve the patient’s quality of life. DFU treatment can be optimized with the help of multidisciplinary approach. Based on many studies, control of glucose levels in blood, antioxidant activity, reduction in cytokine levels, re-epithelialization, collagen formation, migration of fibroblasts are major phases involved in managing DFU. Dehydrozingerone (DHZ), has been known for its anti-inflammatory, antioxidant and wound healing properties. METHODOLOGY: Three months high-fat diet and low dose of streptozotocin-induced type-II diabetic foot ulcer model was used to evaluate the effectiveness of dehydrozingerone. DHZ was given orally to rats for 15 days post wounding. TNF-α, IL-1β and antioxidant parameters like lipid peroxidation, glutathione reductase were estimated. Immunoblotting was done to investigate the effect of DHZ on the expression of ERK, JNK, HSP-27, P38, SIRT-1, NFκB, SMA, VEGF and MMP-9 in skin tissue. Histopathology was performed for analyzing DHZ effect on migration of fibroblasts, formation of epithelium, granulation tissue formation, angiogenesis and collagen formation. RESULTS: DHZ decreased the levels of malondialdehyde, TNF-α, IL-1β and increased glutathione levels in wound tissue. Western blotting results suggested that DHZ activated ERK1/2/JNK/p38 signaling, increased expression of HSP-27, SIRT-1, VEGF, SMA thus facilitating the migration and proliferation of fibroblasts, angiogenesis and decreased inflammation. Masson Trichrome & histopathology showed an increase in collagen, epithelial and granulation tissue formation. CONCLUSION: DHZ significantly accelerates the healing of diabetic foot ulcers in high fat diet fed plus low dose streptozotocin induced type-II diabetic Wistar rats. GRAPHICAL ABSTRACT: [Image: see text] Springer Netherlands 2022-10-25 2023-09 /pmc/articles/PMC10409929/ /pubmed/36280629 http://dx.doi.org/10.1007/s12079-022-00703-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Begum, Farmiza
Manandhar, Suman
Kumar, Gautam
Keni, Raghuvir
Sankhe, Runali
Gurram, Prasada Chowdari
Beegum, Fathima
Teja, Meka Sai
Nandakumar, Krishnadas
Shenoy, Rekha R.
Dehydrozingerone promotes healing of diabetic foot ulcers: a molecular insight
title Dehydrozingerone promotes healing of diabetic foot ulcers: a molecular insight
title_full Dehydrozingerone promotes healing of diabetic foot ulcers: a molecular insight
title_fullStr Dehydrozingerone promotes healing of diabetic foot ulcers: a molecular insight
title_full_unstemmed Dehydrozingerone promotes healing of diabetic foot ulcers: a molecular insight
title_short Dehydrozingerone promotes healing of diabetic foot ulcers: a molecular insight
title_sort dehydrozingerone promotes healing of diabetic foot ulcers: a molecular insight
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10409929/
https://www.ncbi.nlm.nih.gov/pubmed/36280629
http://dx.doi.org/10.1007/s12079-022-00703-0
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