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Protective effects of propolis on ischemia-reperfusion injury in a rat testicular torsion and detorsion model

The torsion model of testis in a rat was adopted for evaluation of possible effects of propolis (Prop) on ischemia-reperfusion (IS/REP) injury. The healthy male Wistar rats (totally 24 animals) were randomized into four groups (n = 6) and animals experienced bilateral testicular torsions as follows:...

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Autores principales: Vakili-Sadeghi, Emad, Najafpour, Alireza, Mohammadi, Rahim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Urmia University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10410105/
https://www.ncbi.nlm.nih.gov/pubmed/37564353
http://dx.doi.org/10.30466/vrf.2023.1972630.3682
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author Vakili-Sadeghi, Emad
Najafpour, Alireza
Mohammadi, Rahim
author_facet Vakili-Sadeghi, Emad
Najafpour, Alireza
Mohammadi, Rahim
author_sort Vakili-Sadeghi, Emad
collection PubMed
description The torsion model of testis in a rat was adopted for evaluation of possible effects of propolis (Prop) on ischemia-reperfusion (IS/REP) injury. The healthy male Wistar rats (totally 24 animals) were randomized into four groups (n = 6) and animals experienced bilateral testicular torsions as follows: In sham group just, laparotomy was performed and in IS group, animals experienced a 3 hr period testicular IS. In IS/REP group, a 3 hr period of IS followed by a 3 hr period of testicular REP for left testis and a one-week testicular REP for right testis were done. In this group animals were gavaged by 1.00 mL normal saline 1 hr before the onset of IS. In IS/REP/ Prop group, the same procedures for IS/REP animals were followed as well as gavage of 1.00 mL Prop extract solution 1 hr before the onset of IS. Analyses of biochemistry, histology, inflammatory biomarkers and sperm parameters were carried out. In IS/REP/Prop group, nitric oxide synthase malondialdehyde, myeloperoxidase and 8-hydroxy-2 deoxyguanine in IS/REP/Prop group were significantly decreased and, superoxide dismutase, total glutathione, glutathione peroxidase, glutathione reductase and glutathione S-transferase were significantly increased compared to the other animals. In IS/REP/Prop group, seminiferous tubules (with normal spermatogenesis) showed all stages of spermatogenic cells with plentiful spermatozoa. Tubular deterioration and atrophy and spermatogenic cell loss in were seen in a limited extent. The mean concentrations of Interleukin-1 beta and tumor necrosis factor alpha in IS/REP/Prop were significantly decreased. Sperm quality was significantly improved by Prop in IS/REP/Prop group. It was concluded that Prop could be supportive in diminishing IS/REP injury in testicular tissue exposed to ischemia.
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spelling pubmed-104101052023-08-10 Protective effects of propolis on ischemia-reperfusion injury in a rat testicular torsion and detorsion model Vakili-Sadeghi, Emad Najafpour, Alireza Mohammadi, Rahim Vet Res Forum Original Article The torsion model of testis in a rat was adopted for evaluation of possible effects of propolis (Prop) on ischemia-reperfusion (IS/REP) injury. The healthy male Wistar rats (totally 24 animals) were randomized into four groups (n = 6) and animals experienced bilateral testicular torsions as follows: In sham group just, laparotomy was performed and in IS group, animals experienced a 3 hr period testicular IS. In IS/REP group, a 3 hr period of IS followed by a 3 hr period of testicular REP for left testis and a one-week testicular REP for right testis were done. In this group animals were gavaged by 1.00 mL normal saline 1 hr before the onset of IS. In IS/REP/ Prop group, the same procedures for IS/REP animals were followed as well as gavage of 1.00 mL Prop extract solution 1 hr before the onset of IS. Analyses of biochemistry, histology, inflammatory biomarkers and sperm parameters were carried out. In IS/REP/Prop group, nitric oxide synthase malondialdehyde, myeloperoxidase and 8-hydroxy-2 deoxyguanine in IS/REP/Prop group were significantly decreased and, superoxide dismutase, total glutathione, glutathione peroxidase, glutathione reductase and glutathione S-transferase were significantly increased compared to the other animals. In IS/REP/Prop group, seminiferous tubules (with normal spermatogenesis) showed all stages of spermatogenic cells with plentiful spermatozoa. Tubular deterioration and atrophy and spermatogenic cell loss in were seen in a limited extent. The mean concentrations of Interleukin-1 beta and tumor necrosis factor alpha in IS/REP/Prop were significantly decreased. Sperm quality was significantly improved by Prop in IS/REP/Prop group. It was concluded that Prop could be supportive in diminishing IS/REP injury in testicular tissue exposed to ischemia. Urmia University Press 2023 2023-07-15 /pmc/articles/PMC10410105/ /pubmed/37564353 http://dx.doi.org/10.30466/vrf.2023.1972630.3682 Text en © 2023 Urmia University. All rights reserved https://creativecommons.org/licenses/by-nc-sa/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.https://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Original Article
Vakili-Sadeghi, Emad
Najafpour, Alireza
Mohammadi, Rahim
Protective effects of propolis on ischemia-reperfusion injury in a rat testicular torsion and detorsion model
title Protective effects of propolis on ischemia-reperfusion injury in a rat testicular torsion and detorsion model
title_full Protective effects of propolis on ischemia-reperfusion injury in a rat testicular torsion and detorsion model
title_fullStr Protective effects of propolis on ischemia-reperfusion injury in a rat testicular torsion and detorsion model
title_full_unstemmed Protective effects of propolis on ischemia-reperfusion injury in a rat testicular torsion and detorsion model
title_short Protective effects of propolis on ischemia-reperfusion injury in a rat testicular torsion and detorsion model
title_sort protective effects of propolis on ischemia-reperfusion injury in a rat testicular torsion and detorsion model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10410105/
https://www.ncbi.nlm.nih.gov/pubmed/37564353
http://dx.doi.org/10.30466/vrf.2023.1972630.3682
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